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[181225_at]

Description F22B5.10 /REP_DB=WormBase Gene ID /WP=CE02196 /TR=Q19714 /GB=CAA90361.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 64      6grp_high1_10_10 > 46      4grp_highest_8_8 > 20      4grp_high_8_8 > 31      6grp_highest_nonorm > 67      6grp_total > 46      4grp_total > 32      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 801
1 2 3 4 range
A 1887.8
(1.8)
P 		1535
(-3)
P 		1974.5
(3)
P 		1912.3
(2.2)
P 		440
B 1590.6
(-3)
P 		1944.9
(-0.1)
P 		2113.1
(1.2)
P 		2335.9
(3)
P 		745
C 1929.4
(-1.6)
P 		2120.9
(1.4)
P 		2226.6
(3)
P 		1836.3
(-3)
P 		390
avr 1802.6
(-3) 		1866.9
(-1.7) 		2104.7
(3) 		2028.2
(1.5) 		302

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 817
1 2 3 4 5 6 range
A 2249.1
(3)
P 		1969.7
(-0.1)
P 		1812.8
(-1.8)
P 		1746.6
(-2.6)
P 		1850.8
(-1.4)
P 		1707.7
(-3)
P 		541
B 1691.8
(-1)
P 		1825.5
(0.6)
P 		1792.6
(0.2)
P 		1814.3
(0.5)
P 		1533.8
(-3)
P 		2017.3
(3)
P 		484
C 1740.3
(-0.6)
P 		1855.3
(0.3)
P 		1621.5
(-1.5)
P 		2009.9
(1.4)
P 		1432.6
(-3)
P 		2212.2
(3)
P 		780
avr 1893.7
(1.6) 		1883.5
(1.5) 		1742.3
(-0.8) 		1856.9
(1) 		1605.7
(-3) 		1979.1
(3) 		373
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 496
1 2 3 4 range
A 687.3
(3)
P 		333.2
(-2.2)
P 		516.2
(0.5)
P 		279.9
(-3)
P 		407
B 507.2
(3)
P 		480.3
(-3)
P 		489.6
(-0.9)
P 		497.8
(0.9)
P 		27
C 638.7
(1.2)
P 		609.8
(0.9)
P 		776
(3)
P 		309.6
(-3)
P 		466
avr 611.1
(3) 		474.4
(-0.3) 		593.9
(2.6) 		362.4
(-3) 		249

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 357
1 2 3 4 5 6 range
A 414.6
(-2.6)
P 		700.5
(3)
P 		394.8
(-3)
P 		443
(-2.1)
P 		558.5
(0.2)
P 		415.8
(-2.6)
P 		306
B 588.7
(-0.1)
P 		607.6
(0.3)
P 		435.3
(-3)
P 		480.5
(-2.1)
P 		623.1
(0.6)
P 		752.2
(3)
P 		317
C 645.1
(1.3)
P 		566.1
(-0.1)
P 		412.7
(-2.8)
P 		404.2
(-3)
P 		463.6
(-1.9)
P 		740.5
(3)
P 		336
avr 549.5
(0.7) 		624.7
(2.7) 		414.3
(-3) 		442.6
(-2.2) 		548.4
(0.6) 		636.2
(3) 		222
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181225_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2730
Sequence Type Exemplar sequence
Representative Public ID F22B5.10 NCBI
Target Description F22B5.10 /REP_DB=WormBase Gene ID /WP=CE02196 /TR=Q19714 /GB=CAA90361.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063385(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063385 NCBI Caenorhabditis elegans F22B5.10 (F22B5.10) mRNA, complete cds. 11/11 None
SNAP00000010408 ENSEMBL cdna:SNAP chromosome:CEL140:II:8467096:8467726:1 11/11 None
GENEFINDER00000010416 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8467096:8467726:1 11/11 None
F22B5.10.2 ENSEMBL cdna:known chromosome:CEL140:II:8467093:8467911:1 gene:F22B5.10 11/11 None
F22B5.10.1 ENSEMBL cdna:known chromosome:CEL140:II:8467096:8467911:1 gene:F22B5.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8467092-8467723(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F22B5.10
Entrez Gene 174352 Entrez gene
SwissProt Q19714 EMBL-EBI
WormBase CE02196 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250687_AT expressed protein at
CANINE:1602889_AT similar to putative membrane protein cfa
CANINE:1584548_AT similar to putative membrane protein cfa
CANINE_2:CFA.9778.1.A1_AT similar to putative membrane protein cfa
CANINE_2:CFAAFFX.20818.1.S1_AT similar to putative membrane protein cfa
CANINE_2:CFAAFFX.20818.1.S1_S_AT similar to putative membrane protein cfa
CANINE_2:CFAAFFX.20820.1.S1_S_AT similar to putative membrane protein cfa
CANINE_2:CFAAFFX.20822.1.S1_AT similar to putative membrane protein cfa
DROSGENOME1:146471_AT dm
DROSOPHILA_2:1634862_AT dm
HG-U133_PLUS_2:210768_X_AT transmembrane and coiled-coil domains 1 hs
HG-U133A:210768_X_AT transmembrane and coiled-coil domains 1 hs
HG-U133A_2:210768_X_AT transmembrane and coiled-coil domains 1 hs
HG-U133_PLUS_2:211098_X_AT transmembrane and coiled-coil domains 1 hs
HG-U133A:211098_X_AT transmembrane and coiled-coil domains 1 hs
HG-U133A_2:211098_X_AT transmembrane and coiled-coil domains 1 hs
HG-U133_PLUS_2:208716_S_AT transmembrane and coiled-coil domains 1 hs
HG-U133A_2:208716_S_AT transmembrane and coiled-coil domains 1 hs
HG-U133A:208716_S_AT transmembrane and coiled-coil domains 1 hs
HG-U133_PLUS_2:208715_AT transmembrane and coiled-coil domains 1 hs
HG-U133A_2:208715_AT transmembrane and coiled-coil domains 1 hs
HG-U133A:208715_AT transmembrane and coiled-coil domains 1 hs
HU35KSUBC:RC_AA598449_AT transmembrane and coiled-coil domains 1 hs
HU35KSUBC:RC_AA252355_AT transmembrane and coiled-coil domains 1 hs
U133_X3P:G6467174_3P_A_AT transmembrane and coiled-coil domains 1 hs
U133_X3P:G12751112_3P_A_AT transmembrane and coiled-coil domains 1 hs
U133_X3P:HS.93832.1.S2_3P_AT transmembrane and coiled-coil domains 1 hs
HG-U95AV2:39116_AT transmembrane and coiled-coil domains 1 hs
HG-U95C:64501_AT transmembrane and coiled-coil domains 1 hs
MU11KSUBA:AA560133_S_AT transmembrane and coiled-coil domains 1 mm
MG-U74AV2:160322_AT transmembrane and coiled-coil domains 1 mm
MOE430A:1423759_A_AT transmembrane and coiled-coil domains 1 mm
MOUSE430A_2:1423759_A_AT transmembrane and coiled-coil domains 1 mm
MOUSE430_2:1423759_A_AT transmembrane and coiled-coil domains 1 mm
MU19KSUBB:TC33926_AT Transmembrane and coiled-coil domains 1 (Tmco1), mRNA mm
RG-U34C:RC_AA819295_AT putative membrane protein rn
RAE230A:1373391_AT putative membrane protein rn
RAT230_2:1373391_AT putative membrane protein rn
RAE230B:1394519_AT Putative membrane protein rn
RAT230_2:1394519_AT Putative membrane protein rn
Protein Similarities
Method ID Description E-Value
blast CAA90361 Hypothetical protein F22B5.10 [Caenorhabditis elegans] ref|NP_495786.1| F22B5.10 [Caenorhabditis elegans] 4.0E-82
blast CAE57637 Hypothetical protein CBG00622 [Caenorhabditis briggsae] 8.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR008559 EMBL-EBI Eukaryotic protein of unknown function DUF841 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495786.1 2 6-28,87-106
F22B5.10.2 2 6-28,87-106
F22B5.10.1 2 6-28,87-106
SNAP00000010408 2 6-28,87-106
GENEFINDER00000010416 2 6-28,87-106
Sequence
Target Sequence 
>C. ELEGANS:181225_AT
gtgattgcctcctgattattgcgatcgcgttcggaactgcccttgccggtgaaggtatca
catggcttcttgtgtatagatccgatcactacaaaagactgaaagctgatatggacaaga
agacgaagaaattagagaagaagaagcaagaagtcggggacaccaatgataaaaatatca
aaagaaagcttgagagagaagaagaacgattaaaggcaactaacagagatatgagcatgt
tcaagatgaaatcaatgttcgcgattggactcgcgttcactgcactcttgtctacattca
actcgatttttgaaggacgtgttgtcgccaaacttccattctatccaattggattcatcc
agggtctttctcatagaaacttgattggcgaagacatgactgattgttcgttcattttcc
tttatattctctgtaccatgaccgttcgtcaaaatctccaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGATTGCCTCCTGATTATTGCGAT 326 485 20 Antisense
TTATTGCGATCGCGTTCGGAACTGC 40 679 35 Antisense
AACTGCCCTTGCCGGTGAAGGTATC 87 141 54 Antisense
GGCTTCTTGTGTATAGATCCGATCA 664 537 83 Antisense
GTTCGCGATTGGACTCGCGTTCACT 25 443 276 Antisense
ACTGCACTCTTGTCTACATTCAACT 362 95 298 Antisense
GAAGGACGTGTTGTCGCCAAACTTC 659 331 331 Antisense
TGGATTCATCCAGGGTCTTTCTCAT 327 551 369 Antisense
GATTGTTCGTTCATTTTCCTTTATA 344 435 421 Antisense
TATATTCTCTGTACCATGACCGTTC 349 661 442 Antisense
TGACCGTTCGTCAAAATCTCCAAAA 320 569 458 Antisense
Sequence Source Affymetrix Proprietary Database