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[181218_at]

Description F41E6.4 /REP_DB=WormBase Gene ID /WP=CE10250 /TR=O16459 /GB=AAB65954.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 36      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 90      6grp_total > 36      4grp_total > 13      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 195
1 2 3 4 range
A 247.9
(-0.3)
P 		225.4
(-1)
P 		161.6
(-3)
P 		356.2
(3)
P 		195
B 255.8
(0.2)
P 		200.6
(-1.7)
P 		163
(-3)
P 		337.9
(3)
P 		175
C 281.3
(3)
P 		210.1
(-3)
P 		210.9
(-2.9)
P 		279.2
(2.8)
P 		71
avr 261.7
(0.4) 		212
(-1.6) 		178.5
(-3) 		324.4
(3) 		146

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 167.5
(1.3)
P 		195.8
(3)
P 		95.3
(-3)
P 		111
(-2.1)
P 		114.4
(-1.9)
P 		163.8
(1.1)
P 		101
B 168.1
(3)
P 		148.8
(1.2)
P 		161.5
(2.4)
P 		102.4
(-3)
P 		110
(-2.3)
P 		156.5
(1.9)
P 		66
C 174
(3)
P 		167
(2.4)
P 		129.2
(-0.7)
P 		124.6
(-1)
P 		100.4
(-3)
P 		165.4
(2.3)
P 		74
avr 169.9
(2.9) 		170.5
(3) 		128.7
(-1) 		112.7
(-2.6) 		108.3
(-3) 		161.9
(2.2) 		62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 90.3
(3)
P 		48.9
(-2.2)
P 		42.2
(-3)
P 		52.1
(-1.8)
P 		48
B 81.6
(3)
P 		49.6
(-1.4)
P 		37.8
(-3)
P 		72
(1.7)
P 		44
C 93.1
(3)
P 		60.4
(-1.3)
P 		73.5
(0.4)
P 		47.1
(-3)
P 		46
avr 88.3
(3) 		53
(-2.7) 		51.2
(-3) 		57.1
(-2) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 30.9
(-1.8)
P 		69.6
(3)
P 		20.8
(-3)
P 		28.2
(-2.1)
P 		34.5
(-1.3)
P 		39.9
(-0.7)
P 		49
B 58.5
(3)
P 		49.5
(1.3)
P 		39.2
(-0.7)
P 		27.1
(-3)
P 		44.7
(0.4)
P 		58.4
(3)
P 		31
C 64.5
(3)
P 		51
(0.9)
P 		32.9
(-1.8)
P 		25
(-3)
P 		32.5
(-1.9)
P 		55.4
(1.6)
P 		40
avr 51.3
(1.9) 		56.7
(3) 		31
(-2.2) 		26.8
(-3) 		37.2
(-0.9) 		51.2
(1.9) 		30
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181218_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11533
Sequence Type Exemplar sequence
Representative Public ID F41E6.4 NCBI
Target Description F41E6.4 /REP_DB=WormBase Gene ID /WP=CE10250 /TR=O16459 /GB=AAB65954.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028738(9), NM_001028740(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028738 NCBI Caenorhabditis elegans F41E6.4a (F41E6.4) mRNA, complete cds. 9/11 None
NM_001028740 NCBI Caenorhabditis elegans F41E6.4c (F41E6.4) mRNA, complete cds. 9/11 None
GENEFINDER00000009444 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8610390:8617667:1 9/11 None
F41E6.4c ENSEMBL cdna:known chromosome:CEL140:V:8610388:8615570:1 gene:F41E6.4 9/11 None
F41E6.4a ENSEMBL cdna:known chromosome:CEL140:V:8610390:8618429:1 gene:F41E6.4 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000009425 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179243 3 2 NM_001028738 180300_at (11), 181218_at (9)
NM_001028739 180300_at (11)
NM_001028740 181218_at (9)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8610390-8612110(+) 73.26 100.0
Public Domain and Genome References
Ensembl F41E6.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAW30664 Hypothetical protein F41E6.4a [Caenorhabditis elegans] ref|NP_001023909.1| F41E6.4a [Caenorhabditis elegans] 0.0
blast AAP13780 Hypothetical protein F41E6.4b [Caenorhabditis elegans] ref|NP_001023910.1| F41E6.4b [Caenorhabditis elegans] 0.0
blast T31878 hypothetical protein F41E6.3 - Caenorhabditis elegans 0.0
blast AAW30665 Hypothetical protein F41E6.4c [Caenorhabditis elegans] ref|NP_001023911.1| F41E6.4c [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006887 EMBL-EBI Protein of unknown function DUF625 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:181218_AT
caggacgcaaccaaccgtgtgaagctttacgttctctgcgatcaacgaatctgggaggac
cgaggtactggtcatgtcgtcactcatcagttatcagctgaagatggagctccgtcgaat
gctggaaatacaatggtcctcgtccgacttgaggggcaaaataaaaacatgctcgagtca
cggattcagatggacacagtctaccaaaaacaacagctcaatcaacccccctcttttaaa
aatttaatgtttttcgaaattatttgtgtccagtttccagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGGACGCAACCAACCGTGTGAAGC 6 203 274 Antisense
CGTGTGAAGCTTTACGTTCTCTGCG 491 245 289 Antisense
GTTCTCTGCGATCAACGAATCTGGG 109 443 304 Antisense
CGAATCTGGGAGGACCGAGGTACTG 650 253 319 Antisense
CGAGGTACTGGTCATGTCGTCACTC 580 245 334 Antisense
TGTCGTCACTCATCAGTTATCAGCT 663 557 348 Antisense
GAAGATGGAGCTCCGTCGAATGCTG 708 337 373 Antisense
GAAATACAATGGTCCTCGTCCGACT 153 363 398 Antisense
GTCCTCGTCCGACTTGAGGGGCAAA 552 469 409 Antisense
AACATGCTCGAGTCACGGATTCAGA 123 137 439 Antisense
AATTATTTGTGTCCAGTTTCCAGAA 287 175 531 Antisense
Sequence Source Affymetrix Proprietary Database