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[181212_at]

Description T04D1.3 /REP_DB=WormBase Gene ID /WP=CE18195 /TR=O61843 /GB=AAC17558.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 19      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_highest_nonorm > 75      6grp_total > 28      4grp_total > 45      6grp_cgc > 02      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 613
1 2 3 4 range
A 1050
(2.4)
P 		1079.1
(3)
P 		765.4
(-3)
P 		901.3
(-0.4)
P 		314
B 924
(0.6)
P 		1041.6
(3)
P 		868.3
(-0.6)
P 		750.2
(-3)
P 		291
C 1363.3
(3)
P 		1015.1
(-2.3)
P 		1038.9
(-2)
P 		971.9
(-3)
P 		391
avr 1112.4
(3) 		1045.3
(1.3) 		890.9
(-2.6) 		874.5
(-3) 		238

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 524
1 2 3 4 5 6 range
A 903
(2.1)
P 		954.7
(3)
P 		624
(-3)
P 		799.6
(0.2)
P 		791.4
(0)
P 		676.1
(-2.1)
P 		331
B 914.9
(3)
P 		749
(-2.4)
P 		741.1
(-2.7)
P 		730.8
(-3)
P 		914.2
(3)
P 		815.4
(-0.2)
P 		184
C 1140.4
(3)
P 		854.5
(-0.3)
P 		735.9
(-1.6)
P 		616.7
(-3)
P 		779.5
(-1.1)
P 		739.9
(-1.6)
P 		524
avr 986.1
(3) 		852.7
(0.2) 		700.3
(-3) 		715.7
(-2.7) 		828.4
(-0.3) 		743.8
(-2.1) 		286
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 319
1 2 3 4 range
A 382.3
(3)
P 		234.2
(-0.5)
P 		200.1
(-1.4)
P 		131.9
(-3)
P 		250
B 294.7
(3)
P 		257.3
(1.3)
P 		201.2
(-1.2)
P 		159.9
(-3)
P 		135
C 451.3
(3)
P 		291.9
(-0.3)
P 		362.1
(1.1)
P 		163.9
(-3)
P 		287
avr 376.1
(3) 		261.1
(-0.1) 		254.5
(-0.3) 		151.9
(-3) 		224

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 299
1 2 3 4 5 6 range
A 166.5
(-2.1)
P 		339.5
(3)
P 		135.9
(-3)
P 		202.8
(-1)
P 		238.8
(0)
P 		164.6
(-2.2)
P 		204
B 318.3
(1.3)
P 		249.3
(-0.8)
P 		180
(-3)
P 		193.6
(-2.6)
P 		371.4
(3)
P 		304.1
(0.9)
P 		191
C 422.7
(3)
P 		260.7
(-0.3)
P 		187.3
(-1.7)
P 		124
(-3)
P 		252.2
(-0.4)
P 		247.7
(-0.5)
P 		299
avr 302.5
(3) 		283.2
(2.1) 		167.7
(-3) 		173.5
(-2.7) 		287.5
(2.3) 		238.8
(0.2) 		135
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181212_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.359
Sequence Type Exemplar sequence
Representative Public ID T04D1.3 NCBI
Target Description T04D1.3 /REP_DB=WormBase Gene ID /WP=CE18195 /TR=O61843 /GB=AAC17558.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059023(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059023 NCBI Caenorhabditis elegans UNCoordinated family member (unc-57) (unc-57) mRNA, complete cds. 11/11 None
SNAP00000041251 ENSEMBL cdna:SNAP chromosome:CEL140:I:4675140:4678853:1 11/11 None
GENEFINDER00000041253 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4675140:4685181:1 11/11 None
T04D1.3 ENSEMBL cdna:known chromosome:CEL140:I:4675136:4679391:1 gene:T04D1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4675139-4678853(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol unc-57
Ensembl T04D1.3
Entrez Gene 172078 Entrez gene
SwissProt O61843 EMBL-EBI
WormBase CE37042 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1586488_AT similar to SH3-containing GRB2-like protein 3 (SH3 domain protein 2C) (EEN-B2) cfa
CANINE_2:CFA.10644.1.A1_AT similar to SH3-containing GRB2-like protein 3 (SH3 domain protein 2C) (EEN-B2) cfa
CANINE_2:CFA.16912.1.S1_AT similar to SH3-containing GRB2-like protein 3 (SH3 domain protein 2C) (EEN-B2) cfa
CANINE_2:CFA.16912.1.S1_S_AT similar to SH3-containing GRB2-like protein 3 (SH3 domain protein 2C) (EEN-B2) cfa
CANINE_2:CFAAFFX.20522.1.S1_S_AT similar to SH3-containing GRB2-like protein 3 (SH3 domain protein 2C) (EEN-B2) cfa
CANINE_2:CFAAFFX.20540.1.S1_AT similar to SH3-containing GRB2-like protein 3 (SH3 domain protein 2C) (EEN-B2) cfa
DROSOPHILA_2:1634452_S_AT endophilin A dm
DROSGENOME1:152475_AT endophilin A dm
CHICKEN:GGA.4424.1.S1_AT SH3-domain GRB2-like 3 gga
HG-U133_PLUS_2:211565_AT SH3-domain GRB2-like 3 hs
HG-U133A:211565_AT SH3-domain GRB2-like 3 hs
HG-U133A_2:211565_AT SH3-domain GRB2-like 3 hs
HG-U133_PLUS_2:205637_S_AT SH3-domain GRB2-like 3 hs
HG-U133A:205637_S_AT SH3-domain GRB2-like 3 hs
HG-U133A_2:205637_S_AT SH3-domain GRB2-like 3 hs
HU35KSUBA:X99664_AT SH3-domain GRB2-like 3 hs
HUGENEFL:X99664_AT SH3-domain GRB2-like 3 hs
HU35KSUBC:RC_F10038_AT SH3-domain GRB2-like 3 hs
U133_X3P:G4506932_3P_A_AT SH3-domain GRB2-like 3 hs
U133_X3P:G2921415_3P_AT SH3-domain GRB2-like 3 hs
HG-U95AV2:37580_AT SH3-domain GRB2-like 3 hs
HG-U95C:56181_AT SH3-domain GRB2-like 3 hs
HG-U133A_2:205636_AT SH3-domain GRB2-like 3 hs
HG-FOCUS:205636_AT SH3-domain GRB2-like 3 hs
HG-U133_PLUS_2:205636_AT SH3-domain GRB2-like 3 hs
HG-U133A:205636_AT SH3-domain GRB2-like 3 hs
MOUSE430_2:1432103_A_AT SH3-domain GRB2-like 3 mm
MOUSE430A_2:1432103_A_AT SH3-domain GRB2-like 3 mm
MOE430A:1432103_A_AT SH3-domain GRB2-like 3 mm
MU11KSUBA:U58887_S_AT SH3-domain GRB2-like 3 mm
MG-U74AV2:101412_AT SH3-domain GRB2-like 3 mm
MG-U74CV2:170009_R_AT SH3-domain GRB2-like 3 mm
RG-U34A:AF009604_AT SH3 domain protein 2 C1 rn
RAE230A:1368931_AT SH3 domain protein 2 C1 rn
RAT230_2:1368931_AT SH3 domain protein 2 C1 rn
RG-U34B:RC_AA943234_AT SH3 domain protein 2 C1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6897 endocytosis inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC17558 Uncoordinated protein 57 [Caenorhabditis elegans] ref|NP_491424.2| UNCoordinated family member (unc-57) [Caenorhabditis elegans] 0.0
blast AAQ96373 endophilin [Caenorhabditis elegans] 0.0
blast AAC17557 Hypothetical protein T04D1.2 [Caenorhabditis elegans] ref|NP_491425.1| T04D1.2 [Caenorhabditis elegans] 0.0
blast AAQ96373 endophilin [Caenorhabditis elegans] 1.0E-180
blast CAE74535 Hypothetical protein CBG22292 [Caenorhabditis briggsae] 1.0E-141
Protein Domains
Database ID Description E-Value
Pfam IPR001452 EMBL-EBI SH3 2.7E-19
Pfam IPR001452 EMBL-EBI SH3 2.7E-19
Pfam IPR001452 EMBL-EBI SH3 1.0E-19
Sequence
Target Sequence 
>C. ELEGANS:181212_AT
cagcaacaacttggccatcgaatcaaagatgcagctgccaggcctcgtgaagagcatgtt
ccattatcagttcttgcaaatgagagcagaactccaagatccagatcgcctgctccatcg
gatatgagtcacaattctacggcagccgcagccgtacgtttttttgtttcagaaaaaatt
gaaaaaatttttcggatttcaattactgaaattttttggaaaatgatttttcggttttcc
ggaaatcctaaaaatcggaaaaatattgggaaaattgacaaaaaatttcaaacttttaaa
aatttggttgatgaaaaaaaaaagtcaattttcttcttaaaacggtttaagatgccacca
caaaatggaggaggaataacacaagcaccaccatcatatcaaggacctccaccaggtgga
cttccacctccactctcccagcagcagaagccacaatgcagagctctattcgattttgat
gctcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGCAACAACTTGGCCATCGAATCA 179 205 715 Antisense
GCCAGGCCTCGTGAAGAGCATGTTC 185 281 751 Antisense
CATGTTCCATTATCAGTTCTTGCAA 436 209 769 Antisense
GAACTCCAAGATCCAGATCGCCTGC 30 349 803 Antisense
TCGCCTGCTCCATCGGATATGAGTC 113 599 820 Antisense
TATGAGTCACAATTCTACGGCAGCC 53 657 837 Antisense
GGAGGAATAACACAAGCACCACCAT 551 515 1084 Antisense
GCACCACCATCATATCAAGGACCTC 392 315 1099 Antisense
GGACCTCCACCAGGTGGACTTCCAC 572 521 1117 Antisense
GAAGCCACAATGCAGAGCTCTATTC 64 345 1161 Antisense
GCTCTATTCGATTTTGATGCTCAAA 221 301 1177 Antisense
Sequence Source Affymetrix Proprietary Database