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[181201_at]

Description F45F2.6 /REP_DB=WormBase Gene ID /WP=CE10528 /TR=Q22977 /GB=AAC48027.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 89      4grp_total > 07      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 87.4
(3)
A 		13.1
(-3)
A 		52.1
(0.1)
A 		81.9
(2.6)
A 		74
B 81.5
(3)
M 		28.6
(-3)
A 		44.7
(-1.2)
A 		60.6
(0.6)
A 		53
C 47
(-2.2)
A 		38.5
(-3)
A 		45.2
(-2.4)
A 		102.8
(3)
A 		64
avr 72
(1.9) 		26.7
(-3) 		47.3
(-0.8) 		81.8
(3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 6.1
(-2.9)
A 		41.2
(0.5)
A 		36.7
(0.1)
A 		9.7
(-2.6)
A 		66.5
(3)
A 		5.5
(-3)
A 		61
B 31.7
(-3)
A 		35.8
(-2.4)
A 		32.1
(-2.9)
A 		40.2
(-1.8)
A 		42.9
(-1.4)
A 		74.6
(3)
A 		43
C 56.5
(-0)
A 		79.7
(3)
A 		33.7
(-3)
A 		56.8
(0)
A 		50.3
(-0.8)
A 		67.1
(1.4)
A 		46
avr 31.4
(-3) 		52.2
(2.7) 		34.2
(-2.2) 		35.6
(-1.8) 		53.2
(3) 		49.1
(1.9) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 31.8
(3)
A 		2.8
(-3)
A 		13.6
(-0.8)
A 		12
(-1.1)
A 		29
B 26
(3)
M 		7.1
(-3)
A 		10.4
(-2)
A 		12.9
(-1.2)
A 		19
C 15.5
(1.3)
A 		11.1
(-3)
A 		15.7
(1.5)
A 		17.3
(3)
A 		6
avr 24.4
(3) 		7
(-3) 		13.2
(-0.9) 		14.1
(-0.6) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 1.1
(-3)
A 		14.6
(1.3)
A 		8
(-0.8)
A 		2.5
(-2.6)
A 		20.1
(3)
A 		1.3
(-2.9)
A 		19
B 11
(-2)
A 		11.9
(-1.8)
A 		7.8
(-3)
A 		10.6
(-2.2)
A 		17.4
(-0.1)
A 		27.8
(3)
A 		20
C 21
(1.7)
A 		24.3
(3)
A 		8.6
(-3)
A 		11.4
(-1.9)
A 		16.3
(-0.1)
A 		22.5
(2.3)
A 		16
avr 11
(-1.2) 		16.9
(2.4) 		8.1
(-3) 		8.2
(-2.9) 		17.9
(3) 		17.2
(2.6) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181201_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11537
Sequence Type Exemplar sequence
Representative Public ID F45F2.6 NCBI
Target Description F45F2.6 /REP_DB=WormBase Gene ID /WP=CE10528 /TR=Q22977 /GB=AAC48027.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072794(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072794 NCBI Caenorhabditis elegans F45F2.6 (F45F2.6) mRNA, complete cds. 11/11 None
SNAP00000030769 ENSEMBL cdna:SNAP chromosome:CEL140:V:8528663:8531169:1 11/11 None
GENEFINDER00000030779 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8528405:8531654:1 11/11 None
F45F2.6 ENSEMBL cdna:known chromosome:CEL140:V:8528663:8531169:1 gene:F45F2.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8528663-8531170(+) 98.74 100.0
Public Domain and Genome References
Gene Title
Ensembl F45F2.6
Entrez Gene 185807 Entrez gene
SwissProt Q22977 EMBL-EBI
WormBase CE10528 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC48027 Hypothetical protein F45F2.6 [Caenorhabditis elegans] ref|NP_505195.1| F45F2.6 [Caenorhabditis elegans] 0.0
blast CAE58381 Hypothetical protein CBG01510 [Caenorhabditis briggsae] 0.0
blast AAC48028 Hypothetical protein F45F2.5 [Caenorhabditis elegans] ref|NP_505196.1| F45F2.5 [Caenorhabditis elegans] 1.0E-143
Protein Domains
Database ID Description E-Value
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 1.0E-126
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 1.3E-103
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 1.0E-126
Pfam IPR004878 EMBL-EBI Protein of unknown function DUF270 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505195.1 9 46-68,78-97,99-116,149-171,178-195,210-232,301-323,348-370,382-404
F45F2.6 9 46-68,78-97,99-116,149-171,178-195,210-232,301-323,348-370,382-404
SNAP00000030769 12 46-68,78-97,99-116,149-171,178-195,210-232,313-335,350-369,389-411,421-443,463-480,495-514
GENEFINDER00000030779 8 57-76,89-111,155-177,184-201,216-238,307-329,354-376,388-410
Sequence
Target Sequence 
>C. ELEGANS:181201_AT
atcgccgagactgtgctattttgtgtctgtttgttagctgtttttggcgctttcataaga
atgcgaaaactccagtacaggttgcacgcgcacggacaagttgttgacgagatccttttg
attgtggggttagcaggggagattgtttattgcagtacaggtttggatttgtatctgaat
ggacgattggaaaacactccaggaacatcttgtcttactgtagtggcatttgtcatgaga
attgttcaagttgttgtgcaaagtgtttttattttagttgcttctaggttgcgcagcttg
tcagccagtaatatatccaaccaacccggaaagcagatagtcacatttttagttgtctgt
aatataaatttatttatttatcacacttttgaaacgatcgaatcaaactttggtttccca
cacaaaatgtcatccgtctattctgccctcctaaatatctcatcgccattagtggtattc
tacagatttcacagttcagcttgcctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGCCGAGACTGTGCTATTTTGTG 17 39 1165 Antisense
GTTAGCTGTTTTTGGCGCTTTCATA 176 447 1197 Antisense
AAAACTCCAGTACAGGTTGCACGCG 215 129 1230 Antisense
GCACGCGCACGGACAAGTTGTTGAC 413 315 1248 Antisense
AACACTCCAGGAACATCTTGTCTTA 57 137 1357 Antisense
CTTCTAGGTTGCGCAGCTTGTCAGC 485 219 1445 Antisense
GCCAGTAATATATCCAACCAACCCG 632 277 1468 Antisense
GAATCAAACTTTGGTTTCCCACACA 195 327 1564 Antisense
CAAAATGTCATCCGTCTATTCTGCC 675 189 1587 Antisense
AAATATCTCATCGCCATTAGTGGTA 655 115 1617 Antisense
CAGATTTCACAGTTCAGCTTGCCTT 221 207 1647 Antisense
Sequence Source Affymetrix Proprietary Database