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[181193_at]

Description T21H3.4 /REP_DB=WormBase Gene ID /WP=CE13904 /TR=O16304 /GB=AAB65365.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 16      4grp_total > 43      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 94.3
(0.8)
A 		98.6
(1.3)
A 		58
(-3)
A 		114.7
(3)
A 		57
B 81.6
(-0.5)
A 		139.7
(3)
A 		101.9
(0.8)
A 		38.7
(-3)
A 		101
C 74.1
(-3)
A 		113.8
(2.6)
A 		105.8
(1.5)
A 		116.6
(3)
A 		43
avr 83.3
(-3) 		117.4
(3) 		88.6
(-2.1) 		90
(-1.8) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 136.7
(3)
A 		76.5
(-0.9)
A 		89.3
(-0)
A 		51.1
(-2.5)
A 		47.8
(-2.7)
A 		43.4
(-3)
A 		93
B 101.9
(3)
A 		88.8
(2.1)
A 		21
(-2.4)
A 		11.6
(-3)
A 		32.7
(-1.6)
A 		38.8
(-1.2)
A 		90
C 88.3
(1.3)
A 		115.8
(3)
A 		93
(1.6)
A 		40.1
(-1.8)
A 		48.5
(-1.3)
A 		20.9
(-3)
A 		95
avr 109
(3) 		93.7
(1.8) 		67.8
(-0.3) 		34.3
(-3) 		43
(-2.3) 		34.4
(-3) 		75
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 34.3
(3)
A 		21.4
(-1.1)
A 		15.2
(-3)
A 		16.8
(-2.5)
A 		19
B 26
(1.1)
A 		34.5
(3)
A 		23.6
(0.5)
A 		8.2
(-3)
A 		26
C 24.5
(-1.3)
A 		32.7
(1.5)
A 		36.9
(3)
A 		19.7
(-3)
A 		17
avr 28.3
(2.5) 		29.5
(3) 		25.2
(1.2) 		14.9
(-3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 25.2
(2.3)
A 		27.2
(3)
A 		19.5
(0.2)
A 		13
(-2.1)
A 		14.4
(-1.6)
A 		10.6
(-3)
A 		17
B 35.5
(3)
A 		29.5
(1.9)
A 		5.1
(-2.6)
A 		3.1
(-3)
A 		13.3
(-1.1)
A 		14.5
(-0.9)
A 		32
C 32.7
(2.4)
A 		35.3
(3)
A 		23.7
(0.5)
A 		8.1
(-2.8)
A 		15.7
(-1.2)
A 		7
(-3)
A 		28
avr 31.1
(3) 		30.7
(2.9) 		16.1
(-0.9) 		8.1
(-3) 		14.5
(-1.3) 		10.7
(-2.3) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181193_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15513
Sequence Type Exemplar sequence
Representative Public ID T21H3.4 NCBI
Target Description T21H3.4 /REP_DB=WormBase Gene ID /WP=CE13904 /TR=O16304 /GB=AAB65365.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070984(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070984 NCBI Caenorhabditis elegans T21H3.4 (T21H3.4) mRNA, complete cds. 11/11 None
GENEFINDER00000016918 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1141675:1146148:1 11/11 None
T21H3.4 ENSEMBL cdna:known chromosome:CEL140:V:1141600:1146148:1 gene:T21H3.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000016908 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1141599-1146148(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T21H3.4
Entrez Gene 188704 Entrez gene
SwissProt O16304 EMBL-EBI
WormBase CE35780 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB65365 Hypothetical protein T21H3.4 [Caenorhabditis elegans] ref|NP_503385.2| T21H3.4 [Caenorhabditis elegans] 0.0
blast CAE58026 Hypothetical protein CBG01100 [Caenorhabditis briggsae] 1.0E-162
blast AAB65365 Hypothetical protein T21H3.4 [Caenorhabditis elegans] ref|NP_503385.2| T21H3.4 [Caenorhabditis elegans] 1.0E-125
blast CAE58026 Hypothetical protein CBG01100 [Caenorhabditis briggsae] 3.0E-83
blast CAE57551 Hypothetical protein CBG00529 [Caenorhabditis briggsae] 1.0E-23
blast CAE59642 Hypothetical protein CBG03056 [Caenorhabditis briggsae] 6.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 2.9E-13
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 2.2E-17
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503385.2 1 472-494
T21H3.4 1 472-494
GENEFINDER00000016918 1 374-396
Sequence
Target Sequence 
>C. ELEGANS:181193_AT
ggcgtgatttatactccaagcaaggcatttcgatttgcaaattcaaaccacgtgcatttc
aaatgcatgatttccgtgtgttcagccgtcgatccctcatgtcgaaatagtgtgcctccg
aaatgcaaaaatgggttggcgaagaaaaagcggcgacagctgcccgaggagatgacgatc
gagcagagattgcttagaattcatgagttaatgaaagtgaaaagagagaatattactgtg
gaagttggacggcccacgaaaaagcttttgagtcatggagcagtaccattggaagtggaa
gaggtttccttaaatgtggtgaatagagaatacgaaatagaaaaacttgagcacattcta
cgatcctatcaaatatggacttggattctttgcgttctcaatgtttttctgacggtaata
tgtgttttgatggcattccgggtgctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCGTGATTTATACTCCAAGCAAGG 414 541 1045 Antisense
AATTCAAACCACGTGCATTTCAAAT 104 177 1084 Antisense
ATGCATGATTTCCGTGTGTTCAGCC 450 43 1107 Antisense
CCGTCGATCCCTCATGTCGAAATAG 452 265 1130 Antisense
GAAATAGTGTGCCTCCGAAATGCAA 575 361 1148 Antisense
CGACAGCTGCCCGAGGAGATGACGA 157 253 1198 Antisense
TGGACGGCCCACGAAAAAGCTTTTG 6 547 1290 Antisense
ACTTGAGCACATTCTACGATCCTAT 143 105 1389 Antisense
TGGACTTGGATTCTTTGCGTTCTCA 513 547 1420 Antisense
GCGTTCTCAATGTTTTTCTGACGGT 592 291 1436 Antisense
GTTTTGATGGCATTCCGGGTGCTGA 102 445 1468 Antisense
Sequence Source Affymetrix Proprietary Database