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[181188_at]

Description C35B1.4 /REP_DB=WormBase Gene ID /WP=CE16902 /TR=O45058 /GB=AAC02560.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 34      6grp_total_sub > Hat      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 8.9
(-2.7)
A 		62.7
(3)
A 		5.7
(-3)
A 		27.7
(-0.7)
A 		57
B 8.4
(-3)
A 		17.9
(-1.6)
A 		49.3
(3)
A 		33.7
(0.7)
A 		41
C 29.1
(-1.3)
A 		72.2
(3)
A 		11.6
(-3)
A 		42.8
(0.1)
A 		61
avr 15.5
(-3) 		50.9
(3) 		22.2
(-1.9) 		34.7
(0.3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 547
1 2 3 4 5 6 range
A 8.3
(-3)
A 		5.7
(-3)
A 		22.2
(-2.8)
A 		22.6
(-2.8)
A 		487.5
(3)
P 		140.9
(-1.3)
P 		482
B 16.4
(-2.9)
A 		21.8
(-2.8)
A 		4.5
(-3)
A 		15
(-2.9)
A 		551.2
(3)
P 		112.5
(-1.8)
P 		547
C 4.3
(-3)
A 		11.2
(-2.9)
A 		8.1
(-3)
A 		9.1
(-2.9)
A 		541.6
(3)
P 		119
(-1.7)
P 		537
avr 9.7
(-3) 		12.9
(-3) 		11.6
(-3) 		15.6
(-2.9) 		526.8
(3) 		124.1
(-1.7) 		517
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 3.3
(-2.1)
A 		13.6
(3)
A 		1.5
(-3)
A 		4
(-1.8)
A 		12
B 2.7
(-3)
A 		4.4
(-1.8)
A 		11.4
(3)
A 		7.2
(0.1)
A 		9
C 9.6
(-1)
A 		20.8
(3)
A 		4
(-3)
A 		7.2
(-1.9)
A 		17
avr 5.2
(-3) 		12.9
(3) 		5.6
(-2.7) 		6.1
(-2.3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 223
1 2 3 4 5 6 range
A 1.5
(-3)
A 		2
(-3)
A 		4.8
(-2.9)
A 		5.7
(-2.8)
A 		147.1
(3)
P 		34.3
(-1.6)
P 		146
B 5.7
(-2.9)
A 		7.2
(-2.8)
A 		1.1
(-3)
A 		4
(-2.9)
A 		223.9
(3)
P 		41.9
(-1.9)
P 		223
C 1.6
(-3)
A 		3.4
(-2.9)
A 		2.1
(-3)
A 		1.8
(-3)
A 		175.2
(3)
P 		39.8
(-1.7)
P 		174
avr 2.9
(-3) 		4.2
(-2.9) 		2.7
(-3) 		3.8
(-3) 		182.1
(3) 		38.7
(-1.8) 		179
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181188_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10601
Sequence Type Exemplar sequence
Representative Public ID C35B1.4 NCBI
Target Description C35B1.4 /REP_DB=WormBase Gene ID /WP=CE16902 /TR=O45058 /GB=AAC02560.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068075(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068075 NCBI Caenorhabditis elegans C35B1.4 (C35B1.4) mRNA, complete cds. 11/11 None
SNAP00000043227 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4079807:4080139:1 11/11 None
GENEFINDER00000043235 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4079807:4080139:1 11/11 None
C35B1.4 ENSEMBL cdna:known chromosome:CEL140:IV:4079807:4080194:1 gene:C35B1.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4079812-4080145(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C35B1.4
Entrez Gene 177166 Entrez gene
SwissProt O45058 EMBL-EBI
WormBase CE16902 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC02560 Hypothetical protein C35B1.4 [Caenorhabditis elegans] ref|NP_500476.1| C35B1.4 [Caenorhabditis elegans] 4.0E-54
Sequence
Target Sequence 
>C. ELEGANS:181188_AT
gtggtgtttcattcgattcactgtgtaacagatcctcctcgagccagaatcatttctctt
ccgggcaccgtggaaggagggcatatcgatgtgaccggacagtataacccagaaacgcca
agaacatccgaagaagaatggtcgggattcccaacatgggagccagaagcatcaggattc
ccaagcacggatggagagcaagaatggtccggcttccctaccatagagcccgaagcttcc
gaattttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGTGTTTCATTCGATTCACTGTG 392 489 46 Antisense
TCACTGTGTAACAGATCCTCCTCGA 161 627 63 Antisense
CGAGCCAGAATCATTTCTCTTCCGG 162 251 85 Antisense
GGCACCGTGGAAGGAGGGCATATCG 687 535 109 Antisense
GGAGGGCATATCGATGTGACCGGAC 272 517 121 Antisense
GTGACCGGACAGTATAACCCAGAAA 523 483 136 Antisense
AACCCAGAAACGCCAAGAACATCCG 522 145 151 Antisense
GAAGCATCAGGATTCCCAAGCACGG 492 341 211 Antisense
GGAGAGCAAGAATGGTCCGGCTTCC 400 519 238 Antisense
GCTTCCCTACCATAGAGCCCGAAGC 410 295 257 Antisense
AGAGCCCGAAGCTTCCGAATTTTCA 164 71 270 Antisense
Sequence Source Affymetrix Proprietary Database