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[181187_at]

Description C52B9.4 /REP_DB=WormBase Gene ID /WP=CE04256 /TR=Q22938 /GB=AAK39214.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 70      4grp_highest_8_8 > 35      4grp_high_8_8 > 48      6grp_highest_nonorm > 62      6grp_total > 80      4grp_total > 63      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 322
1 2 3 4 range
A 500.9
(1.5)
P 		424
(-2.7)
P 		528.9
(3)
P 		419.2
(-3)
P 		110
B 674.2
(3)
P 		471.2
(-0.8)
P 		443.1
(-1.3)
P 		352.5
(-3)
P 		322
C 546.4
(2.8)
P 		403.7
(-3)
P 		551.1
(3)
P 		442.6
(-1.4)
P 		147
avr 573.8
(3) 		433
(-2) 		507.7
(0.7) 		404.8
(-3) 		169

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 277
1 2 3 4 5 6 range
A 474.8
(1.2)
P 		511.1
(3)
P 		451.6
(0.1)
P 		481.2
(1.6)
P 		386.9
(-3)
P 		426.6
(-1.1)
P 		124
B 376.3
(-2.3)
P 		496.1
(0.9)
P 		348.4
(-3)
P 		577.1
(3)
P 		412.3
(-1.3)
P 		451.2
(-0.3)
P 		229
C 441.2
(-0.9)
P 		459.3
(-0.6)
P 		478.4
(-0.1)
P 		623.2
(3)
P 		496.3
(0.3)
P 		346.2
(-3)
P 		277
avr 430.8
(-2.1) 		488.8
(0.2) 		426.1
(-2.3) 		560.5
(3) 		431.8
(-2.1) 		408
(-3) 		153
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 182.4
(3)
P 		92
(-1.5)
P 		138.3
(0.8)
P 		61.4
(-3)
P 		121
B 215
(3)
P 		116.4
(-1.2)
P 		102.7
(-1.8)
P 		75.1
(-3)
P 		140
C 180.9
(2.4)
P 		116.1
(-0.9)
P 		192.1
(3)
P 		74.6
(-3)
P 		118
avr 192.8
(3) 		108.2
(-1.1) 		144.4
(0.6) 		70.4
(-3) 		122

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 87.5
(-3)
P 		181.8
(3)
P 		98.4
(-2.3)
P 		122
(-0.8)
P 		116.8
(-1.1)
P 		103.9
(-2)
P 		94
B 130.9
(0.3)
P 		165.1
(2.8)
P 		84.6
(-3)
P 		152.9
(1.9)
P 		167.5
(2.9)
P 		168.2
(3)
P 		84
C 163.5
(3)
P 		140.2
(0.1)
P 		121.8
(-2.3)
P 		125.3
(-1.8)
P 		160.6
(2.6)
P 		115.9
(-3)
P 		48
avr 127.3
(-0.5) 		162.4
(3) 		101.6
(-3) 		133.4
(0.1) 		148.3
(1.6) 		129.3
(-0.3) 		61
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181187_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16344
Sequence Type Exemplar sequence
Representative Public ID C52B9.4 NCBI
Target Description C52B9.4 /REP_DB=WormBase Gene ID /WP=CE04256 /TR=Q22938 /GB=AAK39214.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076328(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076328 NCBI Caenorhabditis elegans C52B9.4 (C52B9.4) mRNA, complete cds. 9/11 None
SNAP00000014548 ENSEMBL cdna:SNAP chromosome:CEL140:X:4260628:4264250:1 9/11 None
GENEFINDER00000014560 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4260628:4264250:1 9/11 None
C52B9.4.1 ENSEMBL cdna:known chromosome:CEL140:X:4260624:4264526:1 gene:C52B9.4 9/11 None
C52B9.4.2 ENSEMBL cdna:known chromosome:CEL140:X:4260626:4264250:1 gene:C52B9.4 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4260628-4264846(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C52B9.4
Entrez Gene 180699 Entrez gene
SwissProt Q22938 EMBL-EBI
WormBase CE30262 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262200_AT expressed protein at
CANINE_2:CFAAFFX.15800.1.S1_S_AT similar to CG17660-PA cfa
DROSOPHILA_2:1629600_AT dm
DROSGENOME1:145594_AT dm
CHICKEN:GGAAFFX.22773.1.S1_S_AT similar to DKFZP564G2022 protein gga
CHICKEN:GGAAFFX.22773.2.S1_AT similar to DKFZP564G2022 protein gga
HG-U133A:212204_AT DKFZP564G2022 protein hs
HG-U133A_2:212204_AT DKFZP564G2022 protein hs
HG-U133_PLUS_2:212204_AT DKFZP564G2022 protein hs
HG-U133A:212202_S_AT DKFZP564G2022 protein hs
HG-U133_PLUS_2:212202_S_AT DKFZP564G2022 protein hs
HG-U133A_2:212202_S_AT DKFZP564G2022 protein hs
HG-U133_PLUS_2:223772_S_AT DKFZP564G2022 protein hs
HG-U133B:223772_S_AT DKFZP564G2022 protein hs
HU35KSUBA:AA173998_AT DKFZP564G2022 protein hs
HU35KSUBB:RC_N31597_S_AT DKFZP564G2022 protein hs
HU35KSUBD:RC_F09351_AT DKFZP564G2022 protein hs
U133_X3P:G13529124_3P_A_AT DKFZP564G2022 protein hs
U133_X3P:HS.16492.0.S2_3P_AT DKFZP564G2022 protein hs
U133_X3P:HS.16492.0.S1_3P_S_AT DKFZP564G2022 protein hs
HG-U95AV2:40437_AT DKFZP564G2022 protein hs
HU35KSUBB:RC_N63753_AT DKFZP564G2022 protein hs
U133_X3P:HS.35009.0.S2_3P_X_AT DKFZP564G2022 protein hs
U133_X3P:HS.35009.0.S2_3P_AT DKFZP564G2022 protein hs
HG-U133_PLUS_2:223771_AT DKFZP564G2022 protein hs
HG-U133B:223771_AT DKFZP564G2022 protein hs
HG-U95C:60129_F_AT DKFZP564G2022 protein hs
HG-U95C:60130_R_AT DKFZP564G2022 protein hs
HG-U95C:58109_AT DKFZP564G2022 protein hs
HG-U95C:61523_AT DKFZP564G2022 protein hs
HG-U95D:69313_R_AT DKFZP564G2022 protein hs
HG-U95E:70641_F_AT DKFZP564G2022 protein hs
HG-U95E:70643_AT DKFZP564G2022 protein hs
MOE430A:1424454_AT RIKEN cDNA A930025J12 gene mm
MOUSE430A_2:1424454_AT RIKEN cDNA A930025J12 gene mm
MOUSE430_2:1424454_AT RIKEN cDNA A930025J12 gene mm
MU19KSUBB:TC29256_AT RIKEN cDNA A930025J12 gene, mRNA (cDNA clone IMAGE:3967046) mm
Protein Similarities
Method ID Description E-Value
blast NP_508729 C52B9.4 [Caenorhabditis elegans] gb|AAK39214.2| Hypothetical protein C52B9.4 [Caenorhabditis elegans] 0.0
blast T28930 hypothetical protein C52B9.4 - Caenorhabditis elegans 0.0
blast CAE68548 Hypothetical protein CBG14380 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR009637 EMBL-EBI Lung seven transmembrane receptor 1.0E-126
Pfam IPR009637 EMBL-EBI Lung seven transmembrane receptor 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508729.2 7 215-237,249-271,295-312,319-341,356-378,398-420,435-452
C52B9.4.1 7 215-237,249-271,295-312,319-341,356-378,398-420,435-452
C52B9.4.2 7 215-237,249-271,295-312,319-341,356-378,398-420,435-452
SNAP00000014548 7 189-211,223-245,269-286,293-315,330-352,372-394,409-426
GENEFINDER00000014560 7 215-237,249-271,295-312,319-341,356-378,398-420,435-452
Sequence
Target Sequence 
>C. ELEGANS:181187_AT
gagtatgatttaccacatcttcccgacttgtcgtgttgattggaaagaactgtgggtcga
cactgctttctggcacgtgctcttctgttttatcctagttgtcattatgattctctggcg
tccatcacaaaataatcaacgatatgctttcacacctcttcttgatgacagtgaagatga
aaacgatcaagacgaaattttcaacgcatttgctcctggatacgatattctcaccgcccg
gacatctggatctggggctgaaactagaaaacttagagaagctgagaaggagcaagagag
aaaagatgaagctattaaggaggacttgaaatggattgaggaaaatattccgacaacact
ggctgatacacttatcggagattttttaacttttaaagttgttggaacttatttggtagc
cgacccagagggtaaaactggtaattttctaaccagtgaaaataaaaacacaaccaaatg
tcactcgcgcaacgttctcccaacgctattgctcaatgtcgcccaattcaccgatttcat
gag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTATGATTTACCACATCTTCCCG 394 399 1251 Antisense
CACATCTTCCCGACTTGTCGTGTTG 283 195 1264 Antisense
AGAACTGTGGGTCGACACTGCTTTC 415 73 1296 Antisense
GCTCTTCTGTTTTATCCTAGTTGTC 268 301 1329 Antisense
GATTCTCTGGCGTCCATCACAAAAT 631 431 1359 Antisense
CTTTCACACCTCTTCTTGATGACAG 30 223 1397 Antisense
TGCTCCTGGATACGATATTCTCACC 570 581 1461 Antisense
GGACATCTGGATCTGGGGCTGAAAC 626 523 1490 Antisense
ATTCCGACAACACTGGCTGATACAC 373 9 1597 Antisense
CCCAACGCTATTGCTCAATGTCGCC 385 257 1749 Antisense
TCGCCCAATTCACCGATTTCATGAG 458 599 1769 Antisense
Sequence Source Affymetrix Proprietary Database