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[181182_at]

Description R12E2.8 /REP_DB=WormBase Gene ID /WP=CE18140 /TR=O61785 /GB=AAC17029.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 44      6grp_total > 30      4grp_total > 25      6grp_high1_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 194
1 2 3 4 range
A 58.8
(-3)
A 		130.2
(0.6)
P 		110.8
(-0.3)
P 		176.4
(3)
P 		118
B 77.3
(-3)
A 		125.7
(2.7)
P 		128.3
(3)
P 		97.8
(-0.6)
P 		51
C 100.7
(-2.8)
A 		151.6
(-0.9)
P 		95.6
(-3)
P 		253
(3)
P 		157
avr 78.9
(-3) 		135.8
(0.5) 		111.6
(-1) 		175.7
(3) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 97.2
(0.1)
A 		105.8
(0.9)
P 		129.1
(3)
P 		126.5
(2.8)
P 		63.2
(-3)
P 		86.3
(-0.9)
P 		66
B 75
(-3)
P 		119.4
(1.6)
P 		115.2
(1.2)
P 		133.1
(3)
P 		84.8
(-2)
P 		99.5
(-0.5)
P 		58
C 89.6
(-1.8)
P 		160.7
(3)
P 		83.9
(-2.2)
P 		111.7
(-0.3)
P 		72.1
(-3)
P 		124.8
(0.6)
P 		89
avr 87.3
(-1.5) 		128.6
(3) 		109.4
(0.9) 		123.8
(2.5) 		73.4
(-3) 		103.5
(0.3) 		55
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 21.4
(-3)
A 		28.3
(2.4)
P 		29
(3)
P 		25.8
(0.5)
P 		8
B 24.7
(-0.7)
A 		31
(3)
P 		29.7
(2.2)
P 		20.8
(-3)
P 		10
C 33.4
(-2.9)
A 		43.6
(3)
P 		33.3
(-3)
P 		42.7
(2.5)
P 		10
avr 26.5
(-3) 		34.3
(3) 		30.7
(0.2) 		29.8
(-0.5) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 17.9
(-3)
A 		37.6
(3)
P 		28.1
(0.1)
P 		32.1
(1.3)
P 		19.1
(-2.6)
P 		21
(-2.1)
P 		20
B 26.1
(-3)
P 		39.8
(3)
P 		28
(-2.2)
P 		35.3
(1)
P 		34.4
(0.6)
P 		37.1
(1.8)
P 		14
C 33.2
(-0.4)
P 		49
(3)
P 		21.4
(-3)
P 		22.5
(-2.8)
P 		23.3
(-2.6)
P 		41.8
(1.4)
P 		28
avr 25.7
(-3) 		42.1
(3) 		25.8
(-2.9) 		30
(-1.4) 		25.6
(-3) 		33.3
(-0.2) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181182_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.624
Sequence Type Exemplar sequence
Representative Public ID R12E2.8 NCBI
Target Description R12E2.8 /REP_DB=WormBase Gene ID /WP=CE18140 /TR=O61785 /GB=AAC17029.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058911(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058911 NCBI Caenorhabditis elegans R12E2.8 (R12E2.8) mRNA, complete cds. 10/11 None
GENEFINDER00000012695 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4154069:4158699:-1 10/11 None
SNAP00000012678 ENSEMBL cdna:SNAP chromosome:CEL140:I:4155595:4161111:-1 10/11 None
R12E2.8 ENSEMBL cdna:known chromosome:CEL140:I:4155595:4158699:-1 gene:R12E2.8 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4155594-4158699(-) 97.85 100.0
Public Domain and Genome References
Gene Title
Ensembl R12E2.8
Entrez Gene 172003 Entrez gene
SwissProt O61785 EMBL-EBI
WormBase CE34360 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491313 INneXin family member (inx-15) [Caenorhabditis elegans] gb|AAC17030.1| Innexin protein 15 [Caenorhabditis elegans] 0.0
blast CAE66708 Hypothetical protein CBG12053 [Caenorhabditis briggsae] 1.0E-166
blast NP_491312 R12E2.8 [Caenorhabditis elegans] gb|AAC17029.3| Hypothetical protein R12E2.8 [Caenorhabditis elegans] 1.0E-149
blast NP_491312 R12E2.8 [Caenorhabditis elegans] gb|AAC17029.3| Hypothetical protein R12E2.8 [Caenorhabditis elegans] 1.0E-145
blast CAE66709 Hypothetical protein CBG12054 [Caenorhabditis briggsae] 1.0E-114
blast CAE66709 Hypothetical protein CBG12054 [Caenorhabditis briggsae] 1.0E-111
blast NP_491312 R12E2.8 [Caenorhabditis elegans] gb|AAC17029.3| Hypothetical protein R12E2.8 [Caenorhabditis elegans] 4.0E-83
blast BAC41519.1 galaxin [Galaxea fascicularis] pir||JC7958 galaxin precursor - reef coral 8.0E-19
Protein Domains
Database ID Description E-Value
Pfam IPR000990 EMBL-EBI Innexin 6.8E-79
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491312.2 1 5-24
R12E2.8 1 5-24
GENEFINDER00000012695 1 5-24
SNAP00000012678 4 24-46,101-123,189-211,270-292
Sequence
Target Sequence 
>C. ELEGANS:181182_AT
agttccgtctatgctgtcaagttggaaccaaatctgaaaactgtacatcgtattccgaac
atcgagaccttatcggtgtggcttgttgtggcagccacgtatacaatacatcgagtcaat
tgtgttgcgatgaaaacgtacacaatcgggacagaggcggtgtcatggctcattcgtgtt
gcgatacacaacctcttcgcctcgatcaaacttgttgtggaggggtgttttgtcaagcta
tcaaaaatttggcaattgatcaaaaaccctttcttgaatgtttctcttgtagaaaatcca
caccagatgatatttgctgcggaaatgccacgtatgacggtggagttcagcaaatttgct
gcggaaattctgtatttgactctacggaattcaattcctgttgccaactaaacaatggaa
catcatcccgtccgtatcactcttcaactcatgtttgctgtgacggtccactagaaaagg
catcgactgtccgagcatgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTTCCGTCTATGCTGTCAAGTTGG 166 63 95 Antisense
GACCTTATCGGTGTGGCTTGTTGTG 559 381 160 Antisense
TGTCATGGCTCATTCGTGTTGCGAT 453 557 255 Antisense
TGTTGCGATACACAACCTCTTCGCC 7 563 271 Antisense
CTCTTCGCCTCGATCAAACTTGTTG 667 227 287 Antisense
AACCCTTTCTTGAATGTTTCTCTTG 517 145 359 Antisense
GGAATTCAATTCCTGTTGCCAACTA 243 533 480 Antisense
AACAATGGAACATCATCCCGTCCGT 115 135 505 Antisense
GTATCACTCTTCAACTCATGTTTGC 162 455 528 Antisense
TCATGTTTGCTGTGACGGTCCACTA 393 621 543 Antisense
AAAGGCATCGACTGTCCGAGCATGT 106 115 570 Antisense
Sequence Source Affymetrix Proprietary Database