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[181174_at]

Description F53G2.7 /REP_DB=WormBase Gene ID /WP=CE11002 /TR=O17245 /GB=AAB71017.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 52      6grp_total > 07      4grp_total > 60      6grp_cgc > 13      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 192
1 2 3 4 range
A 187
(2.3)
P 		171
(1.4)
A 		197.6
(3)
M 		100.8
(-3)
A 		97
B 165.9
(3)
P 		138.8
(1.7)
A 		144.1
(2)
A 		40.7
(-3)
A 		125
C 183
(0.6)
P 		232.3
(3)
P 		212.5
(2)
P 		111
(-3)
P 		121
avr 178.6
(2.6) 		180.7
(2.8) 		184.7
(3) 		84.2
(-3) 		101

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 316
1 2 3 4 5 6 range
A 100
(-1.3)
A 		182.4
(3)
P 		66.6
(-3)
A 		143.2
(1)
P 		81
(-2.3)
M 		157.1
(1.7)
P 		116
B 115
(2.2)
P 		121.7
(2.9)
P 		101.6
(0.8)
P 		123
(3)
P 		64.7
(-3)
A 		75
(-1.9)
P 		58
C 380.7
(3)
P 		134.4
(-1.9)
P 		125.5
(-2.1)
P 		111.9
(-2.4)
A 		79.9
(-3)
A 		99.3
(-2.6)
P 		301
avr 198.6
(3) 		146.2
(0.5) 		97.9
(-1.9) 		126
(-0.5) 		75.2
(-3) 		110.5
(-1.3) 		123
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 65
1 2 3 4 range
A 68.1
(3)
P 		37.1
(-0.5)
A 		51.6
(1.1)
M 		14.8
(-3)
A 		53
B 52.9
(3)
P 		34.3
(0.5)
A 		33.4
(0.4)
A 		8.7
(-3)
A 		44
C 60.6
(1.5)
P 		66.8
(2.2)
P 		74
(3)
P 		18.7
(-3)
P 		55
avr 60.5
(3) 		46.1
(1.1) 		53
(2) 		14.1
(-3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 127
1 2 3 4 5 6 range
A 18.4
(-2.5)
A 		64.9
(3)
P 		14.5
(-3)
A 		36.3
(-0.4)
P 		24.5
(-1.8)
M 		38.3
(-0.2)
P 		50
B 40
(2.8)
P 		40.5
(3)
P 		24.7
(-3)
P 		32.6
(0)
P 		26.3
(-2.4)
A 		28
(-1.7)
P 		16
C 141.1
(3)
P 		41
(-2.1)
P 		32
(-2.5)
P 		22.5
(-3)
A 		25.8
(-2.8)
A 		33.3
(-2.5)
P 		119
avr 66.5
(3) 		48.8
(0.5) 		23.7
(-3) 		30.5
(-2) 		25.5
(-2.7) 		33.2
(-1.7) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181174_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5968
Sequence Type Exemplar sequence
Representative Public ID F53G2.7 NCBI
Target Description F53G2.7 /REP_DB=WormBase Gene ID /WP=CE11002 /TR=O17245 /GB=AAB71017.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061879
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061879 NCBI Caenorhabditis elegans F53G2.7 (F53G2.7) mRNA, complete cds. None
F53G2.7.2 ENSEMBL cdna:known chromosome:CEL140:II:2473523:2478644:-1 gene:F53G2.7 A 
F53G2.7.1 ENSEMBL cdna:known chromosome:CEL140:II:2473523:2479091:-1 gene:F53G2.7 A 
SNAP00000030758 ENSEMBL cdna:SNAP chromosome:CEL140:II:2461194:2481190:-1 None
GENEFINDER00000030760 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2474109:2479077:-1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2474107-2478945(-) 85.19 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mnat-1
Ensembl F53G2.7
Entrez Gene 173602 Entrez gene
SwissProt O17245 EMBL-EBI
WormBase CE31957 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605161_AT similar to CDK-activating kinase assembly factor MAT1 (RING finger protein MAT1) (Menage a trois) (CDK7/cyclin H assembly factor) (p36) (p35) (Cyclin G1 interacting protein) (RING finger protein 66) cfa
CANINE:1598494_AT similar to CDK-activating kinase assembly factor MAT1 (RING finger protein MAT1) (Menage a trois) (CDK7/cyclin H assembly factor) (p36) (p35) (Cyclin G1 interacting protein) (RING finger protein 66) cfa
CANINE_2:CFA.19433.1.S1_S_AT similar to CDK-activating kinase assembly factor MAT1 (RING finger protein MAT1) (Menage a trois) (CDK7/cyclin H assembly factor) (p36) (p35) (Cyclin G1 interacting protein) (RING finger protein 66) cfa
CANINE_2:CFAAFFX.23967.1.S1_S_AT similar to CDK-activating kinase assembly factor MAT1 (RING finger protein MAT1) (Menage a trois) (CDK7/cyclin H assembly factor) (p36) (p35) (Cyclin G1 interacting protein) (RING finger protein 66) cfa
DROSOPHILA_2:1634055_AT dm
DROSGENOME1:142233_AT dm
CHICKEN:GGAAFFX.7567.1.S1_AT similar to menage a trois 1 gga
HG-U133_PLUS_2:203565_S_AT menage a trois 1 (CAK assembly factor) hs
HG-U133A:203565_S_AT menage a trois 1 (CAK assembly factor) hs
HG-FOCUS:203565_S_AT menage a trois 1 (CAK assembly factor) hs
HG-U133A_2:203565_S_AT menage a trois 1 (CAK assembly factor) hs
HG-U95AV2:1688_S_AT menage a trois 1 (CAK assembly factor) hs
HC-G110:1688_S_AT menage a trois 1 (CAK assembly factor) hs
HUGENEFL:X87843_AT menage a trois 1 (CAK assembly factor) hs
U133_X3P:G4505224_3P_A_AT menage a trois 1 (CAK assembly factor) hs
HG-U95AV2:38387_AT menage a trois 1 (CAK assembly factor) hs
U133_X3P:1556828_3P_AT Menage a trois 1 (CAK assembly factor) hs
HG-U133_PLUS_2:1556828_AT Menage a trois 1 (CAK assembly factor) hs
HG-U133_PLUS_2:237593_AT Menage a trois 1 (CAK assembly factor) hs
HG-U133B:237593_AT Menage a trois 1 (CAK assembly factor) hs
HG-U95C:55285_AT Menage a trois 1 (CAK assembly factor) hs
HG-U95D:80294_AT Menage a trois 1 (CAK assembly factor) hs
HU35KSUBD:RC_W85729_AT Menage a trois 1 (CAK assembly factor) hs
U133_X3P:HS.147250.0.A1_3P_AT Menage a trois 1 (CAK assembly factor) hs
MOUSE430A_2:1432177_A_AT menage a trois 1 mm
MOE430A:1432177_A_AT menage a trois 1 mm
MOUSE430_2:1432177_A_AT menage a trois 1 mm
MU11KSUBA:U35249_S_AT menage a trois 1 mm
MG-U74AV2:98979_AT menage a trois 1 mm
MG-U74AV2:161557_AT menage a trois 1 mm
MU19KSUBB:TC34233_AT Menage a trois 1 (Mnat1), mRNA mm
MU19KSUBB:TC34233_G_AT Menage a trois 1 (Mnat1), mRNA mm
RG-U34C:RC_AI104569_AT menage a trois 1 rn
RAE230A:1375956_AT menage a trois 1 rn
RAT230_2:1375956_AT menage a trois 1 rn
YEAST_2:1770114_AT Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7049 cell cycle inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB71016 Transporter of sr proteins protein 1 [Caenorhabditis elegans] ref|NP_494279.3| Transporter of SR proteins family member (tsr-1) [Caenorhabditis elegans] 0.0
blast CAE73069 Hypothetical protein CBG20440 [Caenorhabditis briggsae] 0.0
blast AAB71017 Mnat (menage a trois) tfiih subunit protein 1 [Caenorhabditis elegans] ref|NP_494280.3| F53G2.7 [Caenorhabditis elegans] 1.0E-160
blast AAB71017 Mnat (menage a trois) tfiih subunit protein 1 [Caenorhabditis elegans] ref|NP_494280.3| F53G2.7 [Caenorhabditis elegans] 1.0E-157
blast CAE73070 Hypothetical protein CBG20441 [Caenorhabditis briggsae] 1.0E-137
blast AAB71017 Mnat (menage a trois) tfiih subunit protein 1 [Caenorhabditis elegans] ref|NP_494280.3| F53G2.7 [Caenorhabditis elegans] 1.0E-127
Protein Domains
Database ID Description E-Value
Pfam IPR004575 EMBL-EBI Cdk-activating kinase assembly factor (MAT1) 9.8E-111
Pfam IPR004575 EMBL-EBI Cdk-activating kinase assembly factor (MAT1) 9.8E-111
Pfam IPR008011 EMBL-EBI Complex 1 LYR protein 1.6E-7
Pfam IPR004575 EMBL-EBI Cdk-activating kinase assembly factor (MAT1) 1.1E-26
Pfam IPR004575 EMBL-EBI Cdk-activating kinase assembly factor (MAT1) 7.4E-7
Pfam IPR004575 EMBL-EBI Cdk-activating kinase assembly factor (MAT1) 2.0E-34
Pfam IPR008011 EMBL-EBI Complex 1 LYR protein 2.9E-12
Sequence
Target Sequence 
>C. ELEGANS:181174_AT
ttcgcgattcgaatgtggcggccgaagtaattctggatcgggaacgtaaaaagcaaatcg
aaaaggaactcgaggaaaaggaggaacaggagaaacgaaaacgccgaaataaggagatgt
tacagacgagaaaacgtgccgccgagaatatgtcgtttaatacggtcattcgtgttgccg
gaagggcttatgttcaccaggttggacatgaaaattcggaagaaatcgaaaaaatgcagc
ttttccactcgaaatttcagattttcaagtcaattttccgattctcccccctcgaattgg
ttatcaatggtccaccaatgccaaatgtttcggaaatcgaggctctcggctatctggctc
acattcgacagcccactgcggaatacattgccggcggctacactccaatgctcggatgct
cac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGCGATTCGAATGTGGCGGCCGA 462 697 1190 Antisense
GTGGCGGCCGAAGTAATTCTGGATC 491 489 1204 Antisense
GAAAACGTGCCGCCGAGAATATGTC 331 355 1319 Antisense
AATACGGTCATTCGTGTTGCCGGAA 122 173 1348 Antisense
GCCGGAAGGGCTTATGTTCACCAGG 628 281 1366 Antisense
AATGCAGCTTTTCCACTCGAAATTT 674 165 1422 Antisense
TCCCCCCTCGAATTGGTTATCAATG 100 593 1474 Antisense
GCTATCTGGCTCACATTCGACAGCC 41 305 1538 Antisense
GACAGCCCACTGCGGAATACATTGC 100 369 1556 Antisense
AATACATTGCCGGCGGCTACACTCC 282 171 1571 Antisense
TACACTCCAATGCTCGGATGCTCAC 628 641 1588 Antisense
Sequence Source Affymetrix Proprietary Database