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[181170_s_at]

Description R01H2.6 /REP_DB=WormBase Gene ID /WP=CE25067 /TR=Q21633 /GB=AAA50648.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 95      4grp_highest_8_8 > 10      4grp_high_8_8 > 55      6grp_highest_nonorm > 78      6grp_total > 85      4grp_total > 57      6grp_highest_sub > In      6grp_cgc > 67      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 836
1 2 3 4 range
A 1006.6
(-2.7)
P 		1286.3
(-0.7)
P 		1796.7
(3)
P 		961
(-3)
P 		836
B 979.4
(-3)
P 		1424.8
(1.5)
P 		1577.3
(3)
P 		1027.5
(-2.5)
P 		598
C 1123.4
(-1.3)
P 		1373.6
(2.3)
P 		1423.7
(3)
P 		1004.2
(-3)
P 		420
avr 1036.5
(-2.6) 		1361.6
(0.6) 		1599.2
(3) 		997.6
(-3) 		602

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 778
1 2 3 4 5 6 range
A 1131.8
(-3)
P 		1171.2
(-2.7)
P 		1499.5
(-0.1)
P 		1682.1
(1.4)
P 		1467.6
(-0.3)
P 		1880.2
(3)
P 		748
B 1252.7
(-1.4)
P 		1102.2
(-3)
P 		1149.5
(-2.5)
P 		1386.7
(-0)
P 		1557.8
(1.8)
P 		1672.8
(3)
P 		571
C 1191.9
(-2.9)
P 		1186.9
(-3)
P 		1501.8
(1.1)
P 		1431.3
(0.2)
P 		1631.2
(2.8)
P 		1647.1
(3)
P 		460
avr 1192.1
(-2.6) 		1153.4
(-3) 		1383.6
(-0.6) 		1500
(0.6) 		1552.2
(1.1) 		1733.4
(3) 		580
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 356
1 2 3 4 range
A 366.5
(1.1)
P 		279.2
(-0.5)
P 		469.7
(3)
P 		140.7
(-3)
P 		329
B 312.3
(0.8)
P 		351.9
(2.4)
P 		365.5
(3)
P 		219
(-3)
P 		147
C 371.9
(0.7)
P 		394.9
(1.1)
P 		496.2
(3)
P 		169.3
(-3)
P 		327
avr 350.2
(0.9) 		342
(0.7) 		443.8
(3) 		176.3
(-3) 		268

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 424
1 2 3 4 5 6 range
A 208.6
(-3)
P 		416.5
(2)
P 		326.6
(-0.2)
P 		426.6
(2.2)
P 		442.9
(2.6)
P 		457.8
(3)
P 		249
B 435.9
(-0.3)
P 		366.9
(-1.5)
P 		279.1
(-3)
P 		367.3
(-1.5)
P 		632.9
(3)
P 		623.8
(2.8)
P 		354
C 441.8
(0.5)
P 		362.2
(-1.3)
P 		382.2
(-0.9)
P 		287.8
(-3)
P 		527.8
(2.5)
P 		551.4
(3)
P 		264
avr 362.1
(-2.1) 		381.9
(-1.5) 		329.3
(-3) 		360.6
(-2.1) 		534.5
(2.7) 		544.3
(3) 		215
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181170_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7232
Sequence Type Exemplar sequence
Representative Public ID R01H2.6 NCBI
Target Description R01H2.6 /REP_DB=WormBase Gene ID /WP=CE25067 /TR=Q21633 /GB=AAA50648.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066140(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066140 NCBI Caenorhabditis elegans UBiquitin Conjugating enzyme family member (ubc-18) (ubc-18) mRNA, complete cds. 11/11 None
SNAP00000040676 ENSEMBL cdna:SNAP chromosome:CEL140:III:7067793:7068466:-1 11/11 None
GENEFINDER00000040681 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7067793:7068466:-1 11/11 None
R01H2.6 ENSEMBL cdna:known chromosome:CEL140:III:7067637:7068496:-1 gene:R01H2.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7067790-7068464(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ubc-18
Ensembl R01H2.6
Entrez Gene 175985 Entrez gene
SwissProt Q21633 EMBL-EBI
WormBase CE25067 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1596983_AT similar to Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3) (Ubiquitin carrier protein L3) (UbcM4) cfa
CANINE:1583829_AT similar to Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3) (Ubiquitin carrier protein L3) (UbcM4) cfa
CANINE_2:CFA.11980.1.A1_S_AT similar to Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3) (Ubiquitin carrier protein L3) (UbcM4) cfa
CANINE_2:CFA.6049.1.A1_AT similar to Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3) (Ubiquitin carrier protein L3) (UbcM4) cfa
DROSOPHILA_2:1634533_AT Ubiquitin conjugating enzyme 10 dm
DROSGENOME1:144122_AT Ubiquitin conjugating enzyme 10 dm
CHICKEN:GGAAFFX.12150.1.S1_AT ubiquitin-conjugating enzyme E2L 3 gga
HG-U95AV2:38701_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133_PLUS_2:200682_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133A_2:200682_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-FOCUS:200682_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133A:200682_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133_PLUS_2:200676_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133A:200676_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133A_2:200676_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133_PLUS_2:200684_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133A_2:200684_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133A:200684_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133_PLUS_2:200683_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133A_2:200683_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U133A:200683_S_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U95AV2:223_AT ubiquitin-conjugating enzyme E2L 3 hs
HC-G110:223_AT ubiquitin-conjugating enzyme E2L 3 hs
HU35KSUBD:RC_AA418526_AT ubiquitin-conjugating enzyme E2L 3 hs
HU35KSUBD:RC_H04327_AT ubiquitin-conjugating enzyme E2L 3 hs
HU35KSUBD:RC_AA487058_AT ubiquitin-conjugating enzyme E2L 3 hs
HUGENEFL:S81003_AT ubiquitin-conjugating enzyme E2L 3 hs
U133_X3P:G4507788_3P_X_AT ubiquitin-conjugating enzyme E2L 3 hs
U133_X3P:HS.108104.0.S3_3P_A_AT ubiquitin-conjugating enzyme E2L 3 hs
U133_X3P:HS.108104.0.S2_3P_A_AT ubiquitin-conjugating enzyme E2L 3 hs
U133_X3P:HS.108104.0.S1_3P_A_AT ubiquitin-conjugating enzyme E2L 3 hs
HG-U95C:64361_S_AT ubiquitin-conjugating enzyme E2L 3 hs
MOE430A:1448879_AT ubiquitin-conjugating enzyme E2L 3 mm
MOUSE430_2:1448879_AT ubiquitin-conjugating enzyme E2L 3 mm
MOUSE430A_2:1448879_AT ubiquitin-conjugating enzyme E2L 3 mm
MOUSE430A_2:1417907_AT ubiquitin-conjugating enzyme E2L 3 mm
MOUSE430_2:1417907_AT ubiquitin-conjugating enzyme E2L 3 mm
MOE430A:1417907_AT ubiquitin-conjugating enzyme E2L 3 mm
MOUSE430A_2:1417908_S_AT ubiquitin-conjugating enzyme E2L 3 mm
MOUSE430_2:1417908_S_AT ubiquitin-conjugating enzyme E2L 3 mm
MOE430A:1417908_S_AT ubiquitin-conjugating enzyme E2L 3 mm
MOUSE430A_2:1448880_AT ubiquitin-conjugating enzyme E2L 3 mm
MOE430A:1448880_AT ubiquitin-conjugating enzyme E2L 3 mm
MOUSE430_2:1448880_AT ubiquitin-conjugating enzyme E2L 3 mm
MU11KSUBB:W78245_S_AT ubiquitin-conjugating enzyme E2L 3 mm
MU11KSUBB:X97042_S_AT ubiquitin-conjugating enzyme E2L 3 mm
MG-U74AV2:97479_AT ubiquitin-conjugating enzyme E2L 3 mm
MU19KSUBC:TC39956_I_AT Ubiquitin-conjugating enzyme E2L 3 (Ube2l3), mRNA mm
RG-U34B:RC_AI007750_AT ubiquitin-conjugating enzyme E2L 3 (predicted) rn
RG-U34C:RC_AI172301_AT ubiquitin-conjugating enzyme E2L 3 (predicted) rn
RG-U34C:RC_AI177960_AT ubiquitin-conjugating enzyme E2L 3 (predicted) rn
RAE230A:1371945_AT ubiquitin-conjugating enzyme E2L 3 (predicted) rn
RAT230_2:1371945_AT ubiquitin-conjugating enzyme E2L 3 (predicted) rn
RAE230A:1371944_AT ubiquitin-conjugating enzyme E2L 3 (predicted) rn
RAT230_2:1371944_AT ubiquitin-conjugating enzyme E2L 3 (predicted) rn
RG-U34C:RC_AI227932_AT Ubiquitin-conjugating enzyme E2L 3 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_498541 UBiquitin Conjugating enzyme family member (ubc-18) [Caenorhabditis elegans] gb|AAA50648.2| Ubiquitin conjugating enzyme protein 18 [Caenorhabditis elegans] 3.0E-87
blast AAZ42342 ubiquitin-conjugating enzyme [Caenorhabditis remanei] 3.0E-86
blast CAE70072 Hypothetical protein CBG16512 [Caenorhabditis briggsae] 2.0E-84
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 1.9E-48
Sequence
Target Sequence 
>C. ELEGANS:181170_S_AT
gcgacacggcgtcttcagaaggaactcggtgatctcaaaaactgtggagtgaaggcgtac
gagaatgttgagtgcgaggagacaaatctcttgaaatggacagttctccttatcccagat
aaggagccatacaacaaaggtgcgttcaaagttggaatcacgttccccgttgactaccca
ttcaagccaccaaaggtcgcgttcgagaccaagatttaccatccaaatgttgatgaggaa
ggaaagttctgtcttccaattgtgacagctgaaaactggaagccagctaccaagaccgag
caagtgatgatggccttgctgtcactgatcaacgaaccagaaccatcccatccaattcgt
gccgacgttgccgaggagttccaaaaggatcacaaaaagttcatgaagaccgccgagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGACACGGCGTCTTCAGAAGGAAC 55 289 19 Antisense
TGAAATGGACAGTTCTCCTTATCCC 110 573 110 Antisense
TCAAAGTTGGAATCACGTTCCCCGT 538 625 164 Antisense
GCCACCAAAGGTCGCGTTCGAGACC 202 279 204 Antisense
CGTTCGAGACCAAGATTTACCATCC 470 247 218 Antisense
GAAAGTTCTGTCTTCCAATTGTGAC 165 361 260 Antisense
ACTGGAAGCCAGCTACCAAGACCGA 587 95 293 Antisense
AGACCGAGCAAGTGATGATGGCCTT 270 71 311 Antisense
GCCTTGCTGTCACTGATCAACGAAC 610 281 331 Antisense
CGACGTTGCCGAGGAGTTCCAAAAG 64 251 381 Antisense
AAAAGTTCATGAAGACCGCCGAGGA 118 131 413 Antisense
Sequence Source Affymetrix Proprietary Database