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[181138_at]

Description F54F12.2 /REP_DB=WormBase Gene ID /WP=CE16115 /TR=O17888 /GB=CAB04463.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 01      4grp_total > 23      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 138.4
(3)
A 		61.3
(-2.2)
A 		50.2
(-3)
A 		115.8
(1.5)
A 		88
B 143.7
(2.4)
A 		84.3
(-2.1)
A 		71.9
(-3)
A 		152.1
(3)
A 		80
C 157.7
(3)
A 		101.3
(-2.7)
A 		97.9
(-3)
A 		125.4
(-0.2)
A 		60
avr 146.6
(3) 		82.3
(-2.3) 		73.3
(-3) 		131.1
(1.7) 		73

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 79.1
(0.3)
A 		104.1
(3)
A 		95.8
(2.1)
P 		103.5
(2.9)
A 		47.8
(-3)
A 		81.3
(0.6)
A 		56
B 103.2
(0.7)
A 		136.1
(3)
A 		86.1
(-0.5)
P 		51.3
(-3)
A 		84
(-0.7)
A 		88.6
(-0.4)
A 		85
C 96.9
(-0.7)
A 		148.6
(3)
A 		83.7
(-1.7)
A 		93.7
(-0.9)
A 		65.2
(-3)
A 		92.7
(-1)
A 		83
avr 93.1
(-0.4) 		129.6
(3) 		88.5
(-0.9) 		82.8
(-1.4) 		65.7
(-3) 		87.5
(-1) 		64
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 50.4
(3)
A 		13.3
(-3)
A 		13.1
(-3)
A 		17
(-2.4)
A 		37
B 45.8
(3)
A 		20.8
(-2.2)
A 		16.7
(-3)
A 		32.4
(0.2)
A 		29
C 52.2
(3)
A 		29.1
(-1.5)
A 		34.1
(-0.5)
A 		21.1
(-3)
A 		31
avr 49.5
(3) 		21.1
(-3) 		21.3
(-3) 		23.5
(-2.5) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 32
1 2 3 4 5 6 range
A 14.6
(-2.9)
A 		37
(3)
A 		20.9
(-1.3)
P 		26.3
(0.2)
A 		14.4
(-3)
A 		19.8
(-1.6)
A 		23
B 35.9
(1.2)
A 		45.3
(3)
A 		20.9
(-1.6)
P 		13.6
(-3)
A 		34.1
(0.9)
A 		33
(0.7)
A 		32
C 35.9
(0.9)
A 		45.3
(3)
A 		21.3
(-2.4)
A 		18.8
(-3)
A 		21.1
(-2.5)
A 		31
(-0.2)
A 		27
avr 28.8
(-0.6) 		42.5
(3) 		21
(-2.6) 		19.6
(-3) 		23.2
(-2.1) 		27.9
(-0.8) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181138_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7949
Sequence Type Exemplar sequence
Representative Public ID F54F12.2 NCBI
Target Description F54F12.2 /REP_DB=WormBase Gene ID /WP=CE16115 /TR=O17888 /GB=CAB04463.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067307(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067307 NCBI Caenorhabditis elegans F54F12.2 (F54F12.2) mRNA, complete cds. 11/11 None
GENEFINDER00000006446 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13046136:13049062:1 11/11 None
F54F12.2 ENSEMBL cdna:known chromosome:CEL140:III:13046136:13048865:1 gene:F54F12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13046131-13048861(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54F12.2
Entrez Gene 186259 Entrez gene
SwissProt O17888 EMBL-EBI
WormBase CE16115 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04463 Hypothetical protein F54F12.2 [Caenorhabditis elegans] ref|NP_499708.1| F54F12.2 [Caenorhabditis elegans] 1.0E-143
blast CAB04463 Hypothetical protein F54F12.2 [Caenorhabditis elegans] ref|NP_499708.1| F54F12.2 [Caenorhabditis elegans] 1.0E-132
blast CAE69576 Hypothetical protein CBG15791 [Caenorhabditis briggsae] 1.0E-114
blast CAE69576 Hypothetical protein CBG15791 [Caenorhabditis briggsae] 1.0E-103
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499708.1 4 140-162,172-194,214-236,256-278
F54F12.2 4 140-162,172-194,214-236,256-278
GENEFINDER00000006446 3 138-160,172-194,209-231
Sequence
Target Sequence 
>C. ELEGANS:181138_AT
aatccacgccaggcagtaacctcaataatatctgctggaatgggggtcgccgcaattaca
gtagccatcatctttttcggaagtctaccagaaagtgcacaaaatgcaaaacaacttgtg
gttgttctggcggtgtatggtattctacaaattgttctggcgactgcattcttcatcaca
tgcatgcagacaacgattgccgtctcgaagggagttaaggacgcatttcaaattgttgtt
ggtcttctggttgctttaatttatgtggctgttacactcatatcgttggctgttggagtc
tttggtttctacaaaaccctgtgcatagtatcatttgtggaatacgaggatctcgaatca
caattctactgtccgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCCACGCCAGGCAGTAACCTCAA 559 165 412 Antisense
ATATCTGCTGGAATGGGGGTCGCCG 238 21 439 Antisense
GTCGCCGCAATTACAGTAGCCATCA 475 475 457 Antisense
GTAGCCATCATCTTTTTCGGAAGTC 500 457 472 Antisense
GTTGTTCTGGCGGTGTATGGTATTC 616 437 532 Antisense
CAAATTGTTCTGGCGACTGCATTCT 584 193 559 Antisense
TGCAGACAACGATTGCCGTCTCGAA 89 577 596 Antisense
AAATTGTTGTTGGTCTTCTGGTTGC 483 115 641 Antisense
GCTGTTACACTCATATCGTTGGCTG 618 295 679 Antisense
GGCTGTTGGAGTCTTTGGTTTCTAC 280 541 699 Antisense
TCGAATCACAATTCTACTGTCCGCC 396 601 764 Antisense
Sequence Source Affymetrix Proprietary Database