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[181131_at]

Description F53G2.8 /REP_DB=WormBase Gene ID /WP=CE11004 /TR=O17248 /GB=AAB71018.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 70      4grp_total > 12      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 69.4
(-1.2)
A 		42.7
(-2.8)
A 		40.2
(-3)
A 		136.9
(3)
P 		97
B 60.5
(-1.6)
A 		84.1
(2)
A 		51.1
(-3)
A 		90.5
(3)
A 		39
C 71.6
(-0.3)
A 		52.7
(-2.1)
A 		42.5
(-3)
A 		108
(3)
A 		66
avr 67.2
(-1) 		59.8
(-1.6) 		44.6
(-3) 		111.8
(3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 51.9
(-0.4)
A 		24.7
(-3)
A 		44.7
(-1.1)
A 		55.8
(-0.1)
A 		42.5
(-1.3)
A 		88.5
(3)
M 		64
B 33.9
(-2.4)
A 		38.2
(-2)
A 		91.7
(3)
A 		58.7
(-0.1)
A 		27.4
(-3)
A 		58
(-0.1)
A 		64
C 42.4
(-1.5)
A 		90.3
(2.4)
A 		33.1
(-2.2)
A 		98.1
(3)
A 		23.5
(-3)
A 		28.5
(-2.6)
A 		75
avr 42.7
(-1.3) 		51.1
(0) 		56.5
(0.8) 		70.9
(3) 		31.1
(-3) 		58.3
(1.1) 		40
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 25.3
(3)
A 		9.3
(-3)
A 		10.5
(-2.6)
A 		20
(1)
P 		16
B 19.3
(2)
A 		20.8
(3)
A 		11.8
(-3)
A 		19.3
(2)
A 		9
C 23.7
(3)
A 		15.2
(-2.7)
A 		14.8
(-3)
A 		18.2
(-0.7)
A 		9
avr 22.8
(3) 		15.1
(-1.4) 		12.4
(-3) 		19.2
(0.9) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 9.6
(-2.6)
A 		8.8
(-3)
A 		9.7
(-2.6)
A 		14.2
(-0.4)
A 		12.8
(-1.1)
A 		21.5
(3)
M 		13
B 11.8
(-2.6)
A 		12.7
(-2.1)
A 		22.3
(3)
A 		15.6
(-0.6)
A 		11.1
(-3)
A 		21.6
(2.6)
A 		11
C 15.7
(-0.6)
A 		27.5
(3)
A 		8.4
(-2.8)
A 		19.7
(0.6)
A 		7.6
(-3)
A 		9.5
(-2.4)
A 		20
avr 12.4
(-1.4) 		16.3
(2) 		13.5
(-0.4) 		16.5
(2.1) 		10.5
(-3) 		17.5
(3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181131_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4579
Sequence Type Exemplar sequence
Representative Public ID F53G2.8 NCBI
Target Description F53G2.8 /REP_DB=WormBase Gene ID /WP=CE11004 /TR=O17248 /GB=AAB71018.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061882(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061882 NCBI Caenorhabditis elegans F53G2.8 (F53G2.8) mRNA, complete cds. 11/11 None
F53G2.8 ENSEMBL cdna:known chromosome:CEL140:II:2483408:2483870:-1 gene:F53G2.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2483406-2483869(-) 59.56 100.0
Public Domain and Genome References
Gene Title
Ensembl F53G2.8
Entrez Gene 186187 Entrez gene
SwissProt O17248 EMBL-EBI
WormBase CE11004 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB71018 Hypothetical protein F53G2.8 [Caenorhabditis elegans] ref|NP_494283.1| F53G2.8 [Caenorhabditis elegans] 3.0E-57
Sequence
Target Sequence 
>C. ELEGANS:181131_AT
agaaatacggtagttctggcggcggctcgcgatgtattcacccagtacacagtcccagct
tcagaattcctaaatggaaatccattttgcgaccatttcggtggcttttgcagttcttca
tcagattcctccacttcttcactgttgtcaattgtacttatggttgctggtgctggattt
ctgggtgctgaaagtccagccgtatttctgaaagagtttacacaactttcattatcaaat
tcgagttctccacaaaatggttacggtagcgccaaaagtacgcaaacaccatctacagta
ccccaaaacacccgatctcacgcggaatgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAATACGGTAGTTCTGGCGGCGG 554 59 22 Antisense
GATGTATTCACCCAGTACACAGTCC 585 409 52 Antisense
CAGTCCCAGCTTCAGAATTCCTAAA 600 207 71 Antisense
TCCATTTTGCGACCATTTCGGTGGC 273 591 102 Antisense
TTTCGGTGGCTTTTGCAGTTCTTCA 366 671 117 Antisense
TCCACTTCTTCACTGTTGTCAATTG 240 589 151 Antisense
TTGTACTTATGGTTGCTGGTGCTGG 455 707 173 Antisense
TGGTGCTGGATTTCTGGGTGCTGAA 422 553 189 Antisense
GCTGAAAGTCCAGCCGTATTTCTGA 20 295 208 Antisense
GTTACGGTAGCGCCAAAAGTACGCA 476 445 281 Antisense
AAACACCCGATCTCACGCGGAATGT 691 125 327 Antisense
Sequence Source Affymetrix Proprietary Database