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[181125_at]

Description E02H9.8 /REP_DB=WormBase Gene ID /WP=CE09125 /TR=Q9TZ99 /GB=AAC68773.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 27      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_total > 27      4grp_total > 54      6grp_cgc > 04      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 204
1 2 3 4 range
A 274.9
(3)
P 		214.8
(0.3)
P 		182.7
(-1.1)
P 		139.4
(-3)
P 		136
B 330.3
(3)
P 		220.7
(-0.5)
P 		238.5
(0)
P 		143.6
(-3)
P 		187
C 343.5
(3)
P 		202.5
(-2.3)
P 		183.8
(-3)
P 		222.5
(-1.5)
P 		160
avr 316.2
(3) 		212.7
(-1.2) 		201.7
(-1.7) 		168.5
(-3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 214.9
(3)
P 		176.1
(0.6)
P 		135.3
(-1.8)
P 		134.6
(-1.9)
P 		116.2
(-3)
P 		141.8
(-1.4)
P 		99
B 198.6
(3)
P 		160.9
(0.1)
P 		119.5
(-3)
P 		139.8
(-1.5)
P 		125.8
(-2.5)
P 		136.3
(-1.7)
P 		79
C 148.4
(2.6)
P 		153.6
(3)
P 		71.1
(-3)
A 		86.3
(-1.9)
P 		129.5
(1.2)
P 		145.9
(2.4)
P 		83
avr 187.3
(3) 		163.5
(1.2) 		108.6
(-3) 		120.2
(-2.1) 		123.8
(-1.8) 		141.3
(-0.5) 		79
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 100.1
(3)
P 		46.6
(-1)
P 		47.8
(-0.9)
P 		20.4
(-3)
P 		80
B 105.3
(3)
P 		54.5
(-1.1)
P 		55.3
(-1)
P 		30.6
(-3)
P 		75
C 113.7
(3)
P 		58.2
(-1.4)
P 		64.1
(-0.9)
P 		37.5
(-3)
P 		76
avr 106.4
(3) 		53.1
(-1.2) 		55.7
(-1) 		29.5
(-3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 39.6
(-1.2)
P 		62.6
(3)
P 		29.5
(-3)
P 		34.1
(-2.2)
P 		35.1
(-2)
P 		34.5
(-2.1)
P 		33
B 69.1
(3)
P 		53.6
(0.7)
P 		29
(-3)
P 		37
(-1.8)
P 		51.1
(0.3)
P 		50.8
(0.3)
P 		40
C 55
(3)
P 		46.9
(1.7)
P 		18.1
(-2.9)
A 		17.4
(-3)
P 		41.9
(0.9)
P 		48.8
(2)
P 		38
avr 54.6
(3) 		54.4
(3) 		25.5
(-3) 		29.5
(-2.2) 		42.7
(0.5) 		44.7
(1) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181125_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7419
Sequence Type Exemplar sequence
Representative Public ID E02H9.8 NCBI
Target Description E02H9.8 /REP_DB=WormBase Gene ID /WP=CE09125 /TR=Q9TZ99 /GB=AAC68773.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065158(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065158 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-121) (nhr-121) mRNA, complete cds. 11/11 None
GENEFINDER00000038961 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2449521:2453808:-1 11/11 None
E02H9.8 ENSEMBL cdna:known chromosome:CEL140:III:2449486:2454793:-1 gene:E02H9.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2449520-2453808(-) 99.43 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nhr-121
Ensembl E02H9.8
Entrez Gene 184003 Entrez gene
SwissProt Q9TZ99 EMBL-EBI
WormBase CE30644 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
3707 steroid hormone receptor activity inferred from electronic annotation QuickGO AmiGO
4879 ligand-dependent nuclear receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAR11991 nuclear receptor NHR-121 [Caenorhabditis elegans] gb|AAC68773.2| Nuclear hormone receptor family protein 121 [Caenorhabditis elegans] ref|NP_497559.2| Nuclear Hormone Receptor family member (nhr-121) [Caenorhabditis elegans] 0.0
blast CAE69477 Hypothetical protein CBG15675 [Caenorhabditis briggsae] 1.0E-171
blast CAE69477 Hypothetical protein CBG15675 [Caenorhabditis briggsae] 1.0E-169
blast CAE69521 Hypothetical protein CBG15730 [Caenorhabditis briggsae] 5.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 2.7E-32
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 2.7E-28
Pfam IPR000536 EMBL-EBI Ligand-binding domain of nuclear hormone receptor 2.7E-32
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 2.7E-28
Sequence
Target Sequence 
>C. ELEGANS:181125_AT
tacgaatggctccgagggtggcggcactttgaattgctcaatatcaaggataaatctgtg
tttctaaggagatgtgtactgtatcacactatattggacccgtcttacctttcttatagg
cttggattacctgaaaaatttgtaatgttcaatggaatgtacgttggggtccaagacggg
gataagaccggatgggaggacgagaaagattgtattacggcggatttgaaaattaaacta
taccgcccgctcatggaccgcctaatcaacgaagtctgcgtctcaatgaaagtcattaac
ctaacatttgtagaatttgtccttctaaaagcattagtatgcttcaaatcttccacaacc
tgtgaagtgtcggtaccactgaaaaagtttatgaatacctacatggataccatattaaga
gcgctgaacgttcattatcagacccttggtatgacaaaagaggatattgctcagaggatt
ggaaatgtcattttgatgatgagcagtatttttgccgttggaatggagtgcttagagtcc
caccagaaaatccaattcttcgacttatggcaactggacgatcttcttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACGAATGGCTCCGAGGGTGGCGGC 166 647 970 Antisense
GGGTGGCGGCACTTTGAATTGCTCA 13 499 985 Antisense
GTATCACACTATATTGGACCCGTCT 326 453 1050 Antisense
CCGTCTTACCTTTCTTATAGGCTTG 489 263 1069 Antisense
TGAAAATTAAACTATACCGCCCGCT 512 569 1196 Antisense
GGACCGCCTAATCAACGAAGTCTGC 349 521 1224 Antisense
GCATTAGTATGCTTCAAATCTTCCA 14 309 1300 Antisense
GAACGTTCATTATCAGACCCTTGGT 223 347 1395 Antisense
AATGGAGTGCTTAGAGTCCCACCAG 326 163 1491 Antisense
CAATTCTTCGACTTATGGCAACTGG 63 181 1522 Antisense
TATGGCAACTGGACGATCTTCTTAT 374 655 1535 Antisense
Sequence Source Affymetrix Proprietary Database