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[181124_at]

Description H12I19.2 /REP_DB=WormBase Gene ID /WP=CE16174 /TR=O45615 /GB=CAB11536.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 34      6grp_cgc > 99      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 12.7
(-2)
A 		15.5
(0.2)
A 		18.9
(3)
A 		11.5
(-3)
A 		7
B 2.9
(-3)
A 		50.2
(3)
M 		8.8
(-2.3)
A 		31.8
(0.7)
A 		47
C 25.4
(-3)
A 		35.7
(-0.7)
A 		39.6
(0.1)
A 		52.7
(3)
A 		27
avr 13.7
(-3) 		33.8
(3) 		22.4
(-0.4) 		32
(2.5) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 15.2
(1.6)
A 		1.6
(-3)
A 		18.6
(2.7)
A 		3.1
(-2.5)
A 		19.4
(3)
A 		5.1
(-1.8)
A 		18
B 19.7
(1.4)
A 		26
(3)
A 		5.8
(-2.2)
A 		11.3
(-0.8)
A 		2.9
(-3)
A 		20.7
(1.6)
A 		23
C 4.2
(-3)
A 		6.1
(-2.4)
A 		14.2
(0.2)
A 		18.5
(1.6)
A 		23
(3)
A 		7.9
(-1.8)
A 		19
avr 13
(-0.1) 		11.2
(-2.7) 		12.9
(-0.2) 		11
(-3) 		15.1
(3) 		11.2
(-2.7) 		4
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 4.6
(2.4)
A 		3.4
(0.2)
A 		4.9
(3)
A 		1.7
(-3)
A 		3
B 0.9
(-3)
A 		12.4
(3)
M 		2
(-2.4)
A 		6.8
(0.1)
A 		12
C 8.4
(-3)
A 		10.3
(-0.9)
A 		13.8
(3)
A 		8.9
(-2.4)
A 		5
avr 4.6
(-3) 		8.7
(3) 		6.9
(0.4) 		5.8
(-1.2) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 2.8
(-0.5)
A 		0.6
(-3)
A 		4
(0.8)
A 		0.8
(-2.8)
A 		5.9
(3)
A 		1.3
(-2.2)
A 		5
B 6.9
(1.6)
A 		8.6
(3)
A 		1.4
(-2.8)
A 		3
(-1.5)
A 		1.2
(-3)
A 		7.7
(2.3)
A 		7
C 1.6
(-3)
A 		1.9
(-2.7)
A 		3.6
(-1)
A 		3.7
(-0.9)
A 		7.5
(3)
A 		2.6
(-2)
A 		6
avr 3.8
(0.2) 		3.7
(0) 		3
(-1.8) 		2.5
(-3) 		4.9
(3) 		3.9
(0.5) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181124_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8701
Sequence Type Exemplar sequence
Representative Public ID H12I19.2 NCBI
Target Description H12I19.2 /REP_DB=WormBase Gene ID /WP=CE16174 /TR=O45615 /GB=CAB11536.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070307(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070307 NCBI Caenorhabditis elegans Serpentine Receptor, class Z family member (srz-31) (srz-31) mRNA, complete cds. 11/11 None
GENEFINDER00000013882 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:14129613:14144932:1 9/11 None
H12I19.2 ENSEMBL cdna:known chromosome:CEL140:IV:14137332:14140263:1 gene:H12I19.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:14137337-14140269(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srz-31
Ensembl H12I19.2
Entrez Gene 186736 Entrez gene
SwissProt O45615 EMBL-EBI
WormBase CE16174 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB11536 Hypothetical protein H12I19.2 [Caenorhabditis elegans] ref|NP_502708.1| Serpentine Receptor, class Z family member (srz-31) [Caenorhabditis elegans] 1.0E-166
blast CAB11536 Hypothetical protein H12I19.2 [Caenorhabditis elegans] ref|NP_502708.1| Serpentine Receptor, class Z family member (srz-31) [Caenorhabditis elegans] 1.0E-101
blast T23080 hypothetical protein H12I19.3 - Caenorhabditis elegans 4.0E-98
blast CAB11539 Hypothetical protein H12I19.1 [Caenorhabditis elegans] ref|NP_502707.2| Serpentine Receptor, class Z family member (srz-42) [Caenorhabditis elegans] 8.0E-63
blast T23080 hypothetical protein H12I19.3 - Caenorhabditis elegans 1.0E-54
blast CAB05676 Hypothetical protein C08F11.9 [Caenorhabditis elegans] ref|NP_502634.2| Serpentine Receptor, class Z family member (srz-29) [Caenorhabditis elegans] 1.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR003845 EMBL-EBI Protein of unknown function DUF216 1.4E-105
Pfam IPR003845 EMBL-EBI Protein of unknown function DUF216 3.6E-30
Pfam IPR003845 EMBL-EBI Protein of unknown function DUF216 2.8E-25
Pfam IPR003845 EMBL-EBI Protein of unknown function DUF216 2.9E-80
Pfam IPR003845 EMBL-EBI Protein of unknown function DUF216 5.4E-29
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502708.1 7 20-42,72-91,106-128,155-177,190-212,233-255,265-287
H12I19.2 7 20-42,72-91,106-128,155-177,190-212,233-255,265-287
GENEFINDER00000013882 18 5-24,34-65,86-108,133-155,162-181,191-213,245-262,282-304,321-343,353-375,382-404,419-441,468-490,503-525,545-567,587-608,629-651,661-678
Sequence
Target Sequence 
>C. ELEGANS:181124_AT
tatcacttattgatcttcctgctcgcagtcggaagattcttgctttacttcttcccaaaa
actaaggaaactgttttatccgctcaaaacattttacaaagaaaagtgaaatacatttat
gcactgtttatagtcaaggatttcatatttctactttgtcttgaaattggaaattcaatt
gggagcaaaagaagagtagaactggctaaaaattgttatgcggccatattcttttctata
aaccttctgataatcatatccgctatcctttacattccgattgtaagaagtgttggaaag
ctcgcgattgtattatctgcaaaacaaagcaaacctcaaaaatacattttatggcagact
atgatagttttcattttcaaaatggtaacttcatttgtgtttatagattttttcctggac
aaagcagctgtcacatttgagcttataccatacatcatactaacagatattgtgataaca
ccgctcataattcaaatttcgtatttgggatttaacaaacgaaatattggaattttatta
tcaacgctcaagattctgccattttcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATCACTTATTGATCTTCCTGCTCG 92 659 364 Antisense
TCCTGCTCGCAGTCGGAAGATTCTT 680 593 380 Antisense
GAAGATTCTTGCTTTACTTCTTCCC 548 337 395 Antisense
TAAGGAAACTGTTTTATCCGCTCAA 602 635 426 Antisense
GGATTTCATATTTCTACTTTGTCTT 512 509 501 Antisense
TTATGCGGCCATATTCTTTTCTATA 287 681 579 Antisense
TAATCATATCCGCTATCCTTTACAT 662 635 614 Antisense
AGAAGTGTTGGAAAGCTCGCGATTG 390 75 649 Antisense
GTCACATTTGAGCTTATACCATACA 491 465 793 Antisense
GATAACACCGCTCATAATTCAAATT 205 423 837 Antisense
AACGCTCAAGATTCTGCCATTTTCG 705 145 906 Antisense
Sequence Source Affymetrix Proprietary Database