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[181117_at]

Description F14B8.2 /REP_DB=WormBase Gene ID /WP=CE02645 /TR=Q19443 /GB=AAA68273.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 71      4grp_highest_8_8 > 15      4grp_high_8_8 > 13      6grp_highest_nonorm > 63      6grp_total > 71      4grp_total > 10      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 760
1 2 3 4 range
A 373.7
(-2.7)
P 		381.5
(-2.6)
P 		339.9
(-3)
P 		919.7
(3)
P 		580
B 279.2
(-3)
P 		375.6
(-2.2)
P 		276.2
(-3)
P 		1036.4
(3)
P 		760
C 399.7
(-2.8)
P 		387
(-2.9)
P 		375.7
(-3)
P 		956.1
(3)
P 		580
avr 350.9
(-2.8) 		381.4
(-2.5) 		330.6
(-3) 		970.7
(3) 		640

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 438
1 2 3 4 5 6 range
A 446.2
(-2.3)
P 		449.1
(-2.2)
P 		776.1
(2.2)
P 		831.7
(3)
P 		523.8
(-1.2)
P 		394
(-3)
P 		438
B 610.5
(-0.8)
P 		606.9
(-0.9)
P 		628.4
(-0.4)
P 		574.6
(-1.6)
P 		779.7
(3)
P 		512.8
(-3)
P 		267
C 584.1
(-0.7)
P 		538.8
(-1.6)
P 		471.4
(-3)
P 		767
(3)
P 		649.7
(0.6)
P 		552.1
(-1.4)
P 		296
avr 546.9
(-1.5) 		531.6
(-1.9) 		625.3
(0.5) 		724.4
(3) 		651.1
(1.2) 		486.3
(-3) 		238
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 157
1 2 3 4 range
A 136
(3)
P 		82.8
(-3)
P 		88.8
(-2.3)
P 		134.6
(2.8)
P 		53
B 89
(-2)
P 		92.8
(-1.9)
P 		64
(-3)
P 		220.9
(3)
P 		157
C 132.3
(-0.5)
P 		111.3
(-3)
P 		130.9
(-0.6)
P 		161.2
(3)
P 		50
avr 119.1
(-1.1) 		95.6
(-2.9) 		94.6
(-3) 		172.2
(3) 		78

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 235
1 2 3 4 5 6 range
A 82.2
(-3)
P 		159.7
(0.6)
P 		169
(1)
P 		210.9
(3)
P 		158.1
(0.5)
P 		95.9
(-2.4)
P 		129
B 212.4
(-0.8)
P 		202
(-1.2)
P 		152.6
(-3)
P 		152.2
(-3)
P 		316.8
(3)
P 		191.2
(-1.6)
P 		165
C 216.5
(3)
P 		164.4
(-0.2)
P 		120
(-3)
P 		154.2
(-0.9)
P 		210.2
(2.6)
P 		184.8
(1)
P 		97
avr 170.4
(-1.3) 		175.4
(-0.9) 		147.2
(-3) 		172.4
(-1.1) 		228.4
(3) 		157.3
(-2.3) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181117_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17046
Sequence Type Exemplar sequence
Representative Public ID F14B8.2 NCBI
Target Description F14B8.2 /REP_DB=WormBase Gene ID /WP=CE02645 /TR=Q19443 /GB=AAA68273.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076767(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076767 NCBI Caenorhabditis elegans F14B8.2 (F14B8.2) mRNA, complete cds. 11/11 None
GENEFINDER00000027549 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6901935:6904975:1 11/11 None
SNAP00000027539 ENSEMBL cdna:SNAP chromosome:CEL140:X:6904727:6904975:1 11/11 None
F14B8.2 ENSEMBL cdna:known chromosome:CEL140:X:6904726:6904975:1 gene:F14B8.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6904726-6904975(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F14B8.2
Entrez Gene 184450 Entrez gene
SwissProt Q19443 EMBL-EBI
WormBase CE02645 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68272 Patterned expression site protein 23 [Caenorhabditis elegans] ref|NP_509167.1| Patterned Expression Site family member (pes-23) [Caenorhabditis elegans] 1.0E-129
blast CAE65816 Hypothetical protein CBG10927 [Caenorhabditis briggsae] 1.0E-119
blast AAA68273 Hypothetical protein F14B8.2 [Caenorhabditis elegans] ref|NP_509168.1| F14B8.2 [Caenorhabditis elegans] 2.0E-32
blast AAA68273 Hypothetical protein F14B8.2 [Caenorhabditis elegans] ref|NP_509168.1| F14B8.2 [Caenorhabditis elegans] 2.0E-31
blast CAE65817 Hypothetical protein CBG10928 [Caenorhabditis briggsae] 1.0E-26
Protein Domains
Database ID Description E-Value
Pfam IPR005828 EMBL-EBI General substrate transporter 1.5E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509168.1 1 19-38
F14B8.2 1 19-38
GENEFINDER00000027549 8 21-43,47-69,126-143,147-169,174-196,206-228,266-288,388-407
SNAP00000027539 1 19-38
Sequence
Target Sequence 
>C. ELEGANS:181117_AT
atgcccagcaaaaactgtgccaaaaaccttcacgcatgtcaatgggagagagacattgct
ttggtatttcttggactaatggttcttttcaacattggacaagttgtgtacatgaacaga
gcacgactctacagactcattcgtcgaggagcagaacaaattccagctgatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCCCAGCAAAAACTGTGCCAAAA 122 41 13 Antisense
TGTGCCAAAAACCTTCACGCATGTC 127 557 28 Antisense
AACCTTCACGCATGTCAATGGGAGA 692 147 37 Antisense
GGGAGAGAGACATTGCTTTGGTATT 637 493 56 Antisense
GACATTGCTTTGGTATTTCTTGGAC 412 371 64 Antisense
TTTCTTGGACTAATGGTTCTTTTCA 409 669 79 Antisense
GGTTCTTTTCAACATTGGACAAGTT 545 499 93 Antisense
GTGTACATGAACAGAGCACGACTCT 212 487 118 Antisense
GAGCACGACTCTACAGACTCATTCG 332 389 131 Antisense
ACAGACTCATTCGTCGAGGAGCAGA 609 107 143 Antisense
GGAGCAGAACAAATTCCAGCTGATG 702 519 160 Antisense
Sequence Source Affymetrix Proprietary Database