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[181082_at]

Description ZK563.5 /REP_DB=WormBase Gene ID /WP=CE05096 /TR=Q23533 /GB=AAA81151.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 83      4grp_highest_8_8 > 63      4grp_high_8_8 > 20      6grp_highest_nonorm > 81      6grp_total > 74      4grp_total > 28      6grp_highest_sub > 04      6grp_high1_sub > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 198.9
(-0.6)
P 		212.1
(0.1)
P 		149.9
(-3)
P 		271.3
(3)
P 		121
B 225.5
(0.8)
P 		256.1
(3)
P 		172.4
(-3)
P 		218.4
(0.3)
P 		84
C 200.8
(-0.9)
P 		196.6
(-1.2)
P 		166.7
(-3)
P 		265.6
(3)
P 		99
avr 208.4
(0.1) 		221.6
(1) 		163
(-3) 		251.8
(3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 148
1 2 3 4 5 6 range
A 288.5
(0.2)
P 		221.5
(-2.5)
P 		339.4
(2.3)
P 		357.4
(3)
P 		209.7
(-3)
P 		273.4
(-0.4)
P 		148
B 235.8
(-3)
P 		272.4
(-0.6)
P 		260.4
(-1.4)
P 		301.4
(1.3)
P 		327.3
(3)
P 		265.7
(-1)
P 		92
C 219.1
(-3)
P 		231.4
(-2.5)
P 		284.1
(-0.1)
P 		354.1
(3)
P 		303.7
(0.8)
P 		327.3
(1.8)
P 		135
avr 247.8
(-2.6) 		241.8
(-3) 		294.6
(0.3) 		337.6
(3) 		280.2
(-0.6) 		288.8
(-0.1) 		96
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 72.4
(3)
P 		46
(-1.8)
P 		39.2
(-3)
P 		39.7
(-2.9)
P 		33
B 71.9
(3)
P 		63.2
(1.4)
P 		40
(-3)
P 		46.5
(-1.8)
P 		32
C 66.5
(3)
P 		56.5
(0.2)
P 		58.1
(0.7)
P 		44.8
(-3)
P 		22
avr 70.3
(3) 		55.2
(-0.4) 		45.8
(-2.5) 		43.7
(-3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 80
1 2 3 4 5 6 range
A 53.2
(-3)
P 		78.8
(1.1)
P 		73.9
(0.3)
P 		90.7
(3)
P 		63.3
(-1.4)
P 		66.6
(-0.9)
P 		38
B 82.1
(-1.4)
P 		90.7
(-0.6)
P 		63.2
(-3)
P 		79.8
(-1.6)
P 		133
(3)
P 		99.1
(0.1)
P 		70
C 81.2
(-1.4)
P 		70.6
(-3)
P 		72.3
(-2.7)
P 		71.2
(-2.9)
P 		98.3
(1.3)
P 		109.6
(3)
P 		39
avr 72.2
(-2.5) 		80
(-0.8) 		69.8
(-3) 		80.6
(-0.7) 		98.2
(3) 		91.8
(1.6) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181082_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18571
Sequence Type Exemplar sequence
Representative Public ID ZK563.5 NCBI
Target Description ZK563.5 /REP_DB=WormBase Gene ID /WP=CE05096 /TR=Q23533 /GB=AAA81151.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029824(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029824 NCBI Caenorhabditis elegans ZK563.5a (ZK563.5) mRNA, complete cds. 10/11 None
ZK563.5a ENSEMBL cdna:known chromosome:CEL140:X:3377188:3378876:-1 gene:ZK563.5 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029825 4/11 Cross Hyb Matching Probes None
SNAP00000043684 8/11 Cross Hyb Matching Probes None
GENEFINDER00000043688 8/11 Cross Hyb Matching Probes None
ZK563.5b 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180626 2 1 NM_001029824 181082_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3377171-3378877(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK563.5
Entrez Gene 180626 Entrez gene
WormBase CE35817 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.5739.1.S1_S_AT similar to HSPC049 protein cfa
DROSOPHILA_2:1636717_AT dm
DROSGENOME1:154392_AT dm
CHICKEN:GGA.7222.2.S1_S_AT similar to HSPC049 protein gga
HG-U133_PLUS_2:222799_AT HSPC049 protein hs
HG-U133B:222799_AT HSPC049 protein hs
HG-U133_PLUS_2:218971_S_AT HSPC049 protein hs
HG-U133A:218971_S_AT HSPC049 protein hs
HG-U133A_2:218971_S_AT HSPC049 protein hs
HG-U95C:63387_AT HSPC049 protein hs
HU35KSUBB:RC_R14977_AT HSPC049 protein hs
HU35KSUBC:RC_AA243585_AT HSPC049 protein hs
U133_X3P:G7661753_3P_A_AT HSPC049 protein hs
U133_X3P:HS.172622.0.S2_3P_X_AT HSPC049 protein hs
HU35KSUBC:RC_N51730_AT HSPC049 protein hs
HU35KSUBD:RC_N62857_S_AT HSPC049 protein hs
U133_X3P:HS.47325.0.A1_3P_AT HSPC049 protein hs
HG-U133_PLUS_2:230888_AT HSPC049 protein hs
HG-U133B:230888_AT HSPC049 protein hs
HG-U95B:51081_AT HSPC049 protein hs
MOUSE430_2:1437819_S_AT RIKEN cDNA 9530020G05 gene mm
MOE430B:1437819_S_AT RIKEN cDNA 9530020G05 gene mm
MG-U74BV2:163659_AT RIKEN cDNA 9530020G05 gene mm
MOE430B:1456181_AT RIKEN cDNA 9530020G05 gene mm
MOUSE430_2:1456181_AT RIKEN cDNA 9530020G05 gene mm
MOE430B:1439124_AT RIKEN cDNA 9530020G05 gene mm
MOUSE430_2:1439124_AT RIKEN cDNA 9530020G05 gene mm
RAE230A:1373746_AT similar to HSPC049 protein (predicted) rn
RAT230_2:1373746_AT similar to HSPC049 protein (predicted) rn
RAE230B:1392696_AT Similar to HSPC049 protein (predicted) rn
RAT230_2:1392696_AT Similar to HSPC049 protein (predicted) rn
RG-U34B:RC_AA850083_AT Similar to HSPC049 protein (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAA81151 Hypothetical protein ZK563.5 [Caenorhabditis elegans] ref|NP_001024995.1| ZK563.5a [Caenorhabditis elegans] 1.0E-129
blast CAE68418 Hypothetical protein CBG14197 [Caenorhabditis briggsae] 1.0E-101
blast NP_001024996 ZK563.5b [Caenorhabditis elegans] 2.0E-49
Sequence
Target Sequence 
>C. ELEGANS:181082_AT
cgacgtggacactttatgcgcaatgtgggagtcttggaatgcccgcgtttttcatagttt
agatacggaaggaataaaacaagcgcaatgttacgaagccagcgcctatcgattatttct
cgttcggtgtgttcaaaagaaaaacatttcaaaatgcaacgaatttttccggaaaatgtc
gtcacttacgttaaacaatccgcaatgggcagattggtttgcatttccctacaaccacca
tgcaaaggacaccgagccatttagaaagtattttgataaaacgtggattgaaatatacta
cgtatcacttcataactttttgtcgacaagtttggcgaacgtttctccaagtgttatcgg
aacgattgtagaaggaatagcaagagatccgactggaaacgatcatgtggatttcgatga
agatttgatagacgacttcgcaatgttctgctcctgtgaaacgtggccactcaaaaccgt
ctctacggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACGTGGACACTTTATGCGCAATG 448 251 180 Antisense
TGTGGGAGTCTTGGAATGCCCGCGT 245 555 203 Antisense
AATGCCCGCGTTTTTCATAGTTTAG 24 167 217 Antisense
AAGCCAGCGCCTATCGATTATTTCT 244 149 275 Antisense
GATTATTTCTCGTTCGGTGTGTTCA 334 429 290 Antisense
AGATTGGTTTGCATTTCCCTACAAC 53 63 390 Antisense
ACCACCATGCAAAGGACACCGAGCC 654 85 413 Antisense
GGCGAACGTTTCTCCAAGTGTTATC 104 541 513 Antisense
AGACGACTTCGCAATGTTCTGCTCC 245 71 609 Antisense
TTCTGCTCCTGTGAAACGTGGCCAC 614 691 625 Antisense
GCCACTCAAAACCGTCTCTACGGAA 233 279 645 Antisense
Sequence Source Affymetrix Proprietary Database