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[181074_at]

Description C52B9.6 /REP_DB=WormBase Gene ID /WP=CE16939 /TR=Q22940 /GB=AAK39218.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 04      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 90      6grp_total > 15      4grp_total > 54      6grp_highest_sub > De      6grp_cgc > 32      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 211.1
(3)
P 		194.1
(1.6)
P 		147.2
(-2.2)
P 		136.7
(-3)
P 		74
B 162.4
(3)
P 		127.4
(-1)
P 		147.8
(1.3)
P 		109.7
(-3)
P 		53
C 200.5
(3)
P 		150.4
(-1.6)
P 		145
(-2.1)
P 		135.7
(-3)
P 		65
avr 191.3
(3) 		157.3
(-0.2) 		146.7
(-1.2) 		127.4
(-3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 151
1 2 3 4 5 6 range
A 209.7
(3)
P 		175.5
(1.6)
P 		159
(1)
P 		128.6
(-0.2)
P 		121.4
(-0.5)
P 		58.5
(-3)
P 		151
B 207.8
(3)
P 		174.6
(1.3)
P 		129.3
(-1)
P 		97.6
(-2.6)
P 		111.6
(-1.9)
P 		89.7
(-3)
P 		118
C 148.2
(0.6)
P 		181.2
(3)
P 		142.5
(0.2)
P 		108.8
(-2.2)
P 		97.3
(-3)
P 		117.5
(-1.6)
P 		84
avr 188.6
(3) 		177.1
(2.3) 		143.6
(0.3) 		111.7
(-1.6) 		110.1
(-1.7) 		88.6
(-3) 		100
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 57
1 2 3 4 range
A 76.9
(3)
P 		42.1
(-0.7)
P 		38.5
(-1)
P 		20
(-3)
P 		57
B 51.8
(3)
P 		31.5
(-1.3)
P 		34.2
(-0.7)
P 		23.4
(-3)
P 		28
C 66.4
(3)
P 		43.2
(-0.2)
P 		50.5
(0.8)
P 		22.9
(-3)
P 		44
avr 65
(3) 		38.9
(-0.7) 		41.1
(-0.3) 		22.1
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 38.6
(0)
P 		62.4
(3)
P 		34.6
(-0.5)
P 		32.6
(-0.7)
P 		36.6
(-0.2)
P 		14.2
(-3)
P 		48
B 72.3
(3)
P 		58.1
(1.2)
P 		31.4
(-2.3)
P 		25.9
(-3)
P 		45.3
(-0.5)
P 		33.5
(-2)
P 		46
C 54.9
(2.9)
P 		55.3
(3)
P 		36.3
(-0.4)
P 		21.9
(-3)
P 		31.5
(-1.3)
P 		39.3
(0.1)
P 		33
avr 55.3
(2.4) 		58.6
(3) 		34.1
(-1.6) 		26.8
(-3) 		37.8
(-0.9) 		29
(-2.6) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181074_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16733
Sequence Type Exemplar sequence
Representative Public ID C52B9.6 NCBI
Target Description C52B9.6 /REP_DB=WormBase Gene ID /WP=CE16939 /TR=Q22940 /GB=AAK39218.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076331(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076331 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-28) (twk-28) mRNA, complete cds. 11/11 None
SNAP00000014551 ENSEMBL cdna:SNAP chromosome:CEL140:X:4271262:4279186:-1 11/11 None
GENEFINDER00000014562 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:4274760:4279186:-1 11/11 None
C52B9.6 ENSEMBL cdna:known chromosome:CEL140:X:4274760:4279186:-1 gene:C52B9.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4274760-4279187(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol twk-28
Ensembl C52B9.6
Entrez Gene 183714 Entrez gene
SwissProt Q22940 EMBL-EBI
WormBase CE35710 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.18516.1.S1_AT similar to potassium channel, subfamily K, member 18 cfa
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6811 ion transport inferred from electronic annotation QuickGO AmiGO
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_508732 TWiK family of potassium channels family member (twk-28) [Caenorhabditis elegans] gb|AAK39218.3| Twik family of potassium channels protein 28 [Caenorhabditis elegans] 0.0
blast AAQ96601 two-P domain K channel [Caenorhabditis elegans] 0.0
blast T28933 hypothetical protein C52B9.6 - Caenorhabditis elegans 0.0
blast NP_508731 MEChanosensory abnormality family member (mec-18) [Caenorhabditis elegans] gb|AAK39217.3| Mechanosensory abnormality protein 18 [Caenorhabditis elegans] pir||T28932 probable 4-coumarate-CoA ligase (EC 6.2.1.12) - Caenorhabditis elegans 0.0
Protein Families
Method ID Description E-Value
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-120
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 1.5E-13
Pfam IPR005821 EMBL-EBI Ion transport protein 1.5E-13
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 3.4E-76
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508732.3 6 58-80,166-188,195-217,286-308,320-337,342-364
C52B9.6 6 58-80,166-188,195-217,286-308,320-337,342-364
SNAP00000014551 7 58-80,174-196,203-225,294-316,328-345,350-372,748-770
GENEFINDER00000014562 6 58-80,166-188,195-217,286-308,320-337,342-364
Sequence
Target Sequence 
>C. ELEGANS:181074_AT
agcattctacttttgttttatcacgatggcaactgtcgggtttggagacattgtgccgaa
cgagcaggtttatgtgttcttcacaatggcttatattatttttgggttgtcattagcaac
tatgtgcattgatcttgctggtactgagtatattcggaaaattcactacttgggtacaaa
aatggaagacgcaaaaggagctgtgatgaccgggctccaagctggagagcatttgttgaa
acatacaggaattgaagtgattaaaacagctggtggaaaacttgtccaagtacgaggcgc
agttctcagttcgaaagaagcaagagagttgggaatgagctatctgctgcaattcaacta
tcagcataaaaatgtgctctacgagccgttaactggaaacttggcaaaagttgtgaagga
agcaaacattaagattctgccagacgacattactgaaaaagacgggtatattgtacaaaa
caagagctatgatcgcccagtgcttggcgagcgaggatttgtccggttgaatggaaatgc
aataaaacgaataatgattccattgaggaaccaggcgatttgtgtaccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCATTCTACTTTTGTTTTATCACG 177 77 936 Antisense
ATCACGATGGCAACTGTCGGGTTTG 217 29 955 Antisense
GGGTTTGGAGACATTGTGCCGAACG 646 495 973 Antisense
ACGAGCAGGTTTATGTGTTCTTCAC 494 93 995 Antisense
TGATGACCGGGCTCCAAGCTGGAGA 654 559 1139 Antisense
TACGAGGCGCAGTTCTCAGTTCGAA 212 647 1226 Antisense
TGAGCTATCTGCTGCAATTCAACTA 402 569 1271 Antisense
GTGCTCTACGAGCCGTTAACTGGAA 443 477 1309 Antisense
CAAGAGCTATGATCGCCCAGTGCTT 605 183 1416 Antisense
GCTTGGCGAGCGAGGATTTGTCCGG 342 305 1437 Antisense
GAGGAACCAGGCGATTTGTGTACCG 323 405 1500 Antisense
Sequence Source Affymetrix Proprietary Database