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[181072_at]

Description C06E8.2 /REP_DB=WormBase Gene ID /WP=CE00725 /TR=Q17737 /GB=AAA50639.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 26      4grp_highest_8_8 > 53      4grp_high_8_8 > 17      6grp_highest_nonorm > 21      6grp_total > 26      4grp_total > 31      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Cup      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 698
1 2 3 4 range
A 1620.1
(3)
P 		1136.7
(-1.5)
P 		982.2
(-3)
P 		1399.3
(0.9)
P 		638
B 1655.5
(3)
P 		957.8
(-3)
P 		968.5
(-2.9)
P 		1367.7
(0.5)
P 		698
C 1641.4
(3)
P 		1062.7
(-2.2)
P 		967.3
(-3)
P 		1269.7
(-0.3)
P 		674
avr 1639
(3) 		1052.4
(-2.3) 		972.7
(-3) 		1345.6
(0.4) 		666

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 980
1 2 3 4 5 6 range
A 1665.1
(3)
P 		1486.3
(1.7)
P 		925.3
(-2.3)
P 		956.5
(-2.1)
P 		910.9
(-2.4)
P 		826.4
(-3)
P 		839
B 1753.9
(3)
P 		1498.4
(1.3)
P 		1089.5
(-1.4)
P 		1089.8
(-1.4)
P 		849
(-3)
P 		987.7
(-2.1)
P 		905
C 1200.2
(1.7)
P 		1317.5
(3)
P 		987.3
(-0.6)
P 		774.3
(-3)
P 		970.7
(-0.8)
P 		1051.1
(0.1)
P 		543
avr 1539.7
(3) 		1434.1
(2) 		1000.7
(-2.1) 		940.2
(-2.7) 		910.2
(-3) 		955.1
(-2.6) 		630
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 385
1 2 3 4 range
A 589.9
(3)
P 		246.8
(-2.3)
P 		256.8
(-2.2)
P 		204.8
(-3)
P 		385
B 527.9
(3)
P 		236.5
(-2.8)
P 		224.4
(-3)
P 		291.5
(-1.7)
P 		304
C 543.3
(3)
P 		305.5
(-1.3)
P 		337.1
(-0.8)
P 		214.1
(-3)
P 		329
avr 553.7
(3) 		262.9
(-2.5) 		272.8
(-2.3) 		236.8
(-3) 		317

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 455
1 2 3 4 5 6 range
A 306.9
(-1.1)
P 		528.6
(3)
P 		201.5
(-3)
P 		242.6
(-2.2)
P 		274.9
(-1.6)
P 		201.2
(-3)
P 		327
B 610.3
(3)
P 		498.8
(1.1)
P 		264.6
(-3)
P 		288.7
(-2.6)
P 		344.9
(-1.6)
P 		368.3
(-1.2)
P 		346
C 444.9
(3)
P 		402
(2.1)
P 		251.3
(-1)
P 		155.7
(-3)
P 		314.1
(0.3)
P 		351.8
(1.1)
P 		289
avr 454
(2.5) 		476.5
(3) 		239.1
(-2.8) 		229
(-3) 		311.3
(-1) 		307.1
(-1.1) 		248
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181072_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7230
Sequence Type Exemplar sequence
Representative Public ID C06E8.2 NCBI
Target Description C06E8.2 /REP_DB=WormBase Gene ID /WP=CE00725 /TR=Q17737 /GB=AAA50639.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066126(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066126 NCBI Caenorhabditis elegans Pim (mammalian oncogene) Related Kinase family member (prk-1) (prk-1) mRNA, complete cds. 11/11 A 
SNAP00000030990 ENSEMBL cdna:SNAP chromosome:CEL140:III:7001443:7002105:1 11/11 None
GENEFINDER00000030992 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7001443:7002105:1 11/11 None
C06E8.3a ENSEMBL cdna:known chromosome:CEL140:III:6996412:7002228:1 gene:C06E8.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171179 4/11 Cross Hyb Matching Probes None
C06E8.3c 4/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175980 3 1 NM_066126 181072_at (11), 190994_at (11)
NM_171179 190994_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7001440-7002103(+) 100.0 100.0
Public Domain and Genome References
Ensembl C06E8.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA50639 Pim (mammalian oncogene) related kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_498527.1| Pim (mammalian oncogene) Related Kinase family member (prk-1) [Caenorhabditis elegans] 0.0
blast CAE70061 Hypothetical protein CBG16496 [Caenorhabditis briggsae] 0.0
blast T15435 hypothetical protein C06E8.3 - Caenorhabditis elegans 0.0
blast AAA50639 Pim (mammalian oncogene) related kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_498527.1| Pim (mammalian oncogene) Related Kinase family member (prk-1) [Caenorhabditis elegans] 2.0E-79
blast CAE70061 Hypothetical protein CBG16496 [Caenorhabditis briggsae] 3.0E-72
Protein Families
Method ID Description E-Value
hanks 1.4.2 AGC group; AGC IV GPCR kinases; bARK2 7.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-52
Sequence
Target Sequence 
>C. ELEGANS:181072_AT
gtctcctcatcatacgacgatcaattcacttcgacttctgatatctaccaatcggcttgt
gaagtgttcccacacacaggctcgtcatcctcactgagcagaagcatgggagcagtcaaa
tcagcatccgcctataatttagttcggatgcgaaagaaatcgagtattttcaaatcattt
gacacagaactcgatccagtttctgaaaacgatcatcaaccattgattcctgagacgtcg
tcagcaacgtgtcagtcaacatacagtaatgatgaagatgtgacaatatcggaaaaagtt
gtatcaccagctcagccactgccaccacctctgtcccctcggaaaggaagccttcaatgc
ttcgaatcagtaccaagatctcgagattctgttatcagaatgtcacgtcatcgtccactt
caatttgcaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTCCTCATCATACGACGATCAAT 585 469 76 Antisense
CACTTCGACTTCTGATATCTACCAA 211 197 102 Antisense
GGCTTGTGAAGTGTTCCCACACACA 404 537 129 Antisense
GCTCGTCATCCTCACTGAGCAGAAG 604 299 155 Antisense
GGGAGCAGTCAAATCAGCATCCGCC 103 495 183 Antisense
CATTTGACACAGAACTCGATCCAGT 107 215 251 Antisense
AACCATTGATTCCTGAGACGTCGTC 411 147 293 Antisense
AAAAAGTTGTATCACCAGCTCAGCC 599 129 368 Antisense
TCTGTCCCCTCGGAAAGGAAGCCTT 443 605 405 Antisense
TCAGAATGTCACGTCATCGTCCACT 58 625 470 Antisense
GTCATCGTCCACTTCAATTTGCAAT 686 465 482 Antisense
Sequence Source Affymetrix Proprietary Database