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[181067_at]

Description C14F5.4 /REP_DB=WormBase Gene ID /WP=CE01783 /TR=Q09972 /GB=AAA68404.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 11      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_total > 23      4grp_total > 60      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 263
1 2 3 4 range
A 390.3
(3)
P 		390.5
(3)
P 		326.8
(-0.9)
P 		293.7
(-3)
P 		97
B 357
(2.2)
P 		379.8
(2.8)
P 		390.4
(3)
P 		127.4
(-3)
P 		263
C 345.1
(2.6)
P 		335.6
(2.4)
P 		358.2
(3)
P 		134.3
(-3)
P 		224
avr 364.1
(2.9) 		368.6
(3) 		358.5
(2.7) 		185.1
(-3) 		184

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 281
1 2 3 4 5 6 range
A 414.2
(3)
P 		293.8
(0)
P 		327.3
(0.9)
P 		355.8
(1.6)
P 		183
(-2.7)
P 		170
(-3)
A 		244
B 386.7
(3)
P 		349.9
(2.1)
P 		330.6
(1.7)
P 		300.5
(1)
P 		146.7
(-2.7)
P 		133.1
(-3)
P 		254
C 328.2
(1)
P 		412.1
(3)
P 		348.6
(1.5)
P 		318.2
(0.7)
P 		165.1
(-3)
P 		179.1
(-2.7)
P 		247
avr 376.4
(3) 		351.9
(2.3) 		335.5
(1.9) 		324.8
(1.6) 		164.9
(-2.9) 		160.7
(-3) 		216
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 120
1 2 3 4 range
A 142.1
(3)
P 		84.8
(-0.5)
P 		85.4
(-0.4)
P 		43
(-3)
P 		99
B 113.8
(3)
P 		93.8
(1.6)
P 		90.5
(1.4)
P 		27.1
(-3)
P 		87
C 114.2
(2.4)
P 		96.5
(1.3)
P 		124.9
(3)
P 		22.6
(-3)
P 		102
avr 123.4
(3) 		91.7
(0.9) 		100.3
(1.5) 		30.9
(-3) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 76.4
(0.3)
P 		104.5
(3)
P 		71.3
(-0.2)
P 		90.2
(1.6)
P 		55.2
(-1.7)
P 		41.4
(-3)
A 		63
B 134.6
(3)
P 		116.5
(1.7)
P 		80.3
(-0.8)
P 		79.6
(-0.9)
P 		59.6
(-2.3)
P 		49.6
(-3)
P 		85
C 121.7
(2.7)
P 		125.7
(3)
P 		88.7
(-0.1)
P 		64
(-2.1)
P 		53.4
(-3)
P 		60
(-2.5)
P 		72
avr 110.9
(2.6) 		115.6
(3) 		80.1
(-0.3) 		77.9
(-0.5) 		56.1
(-2.5) 		50.3
(-3) 		65
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181067_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17081
Sequence Type Exemplar sequence
Representative Public ID C14F5.4 NCBI
Target Description C14F5.4 /REP_DB=WormBase Gene ID /WP=CE01783 /TR=Q09972 /GB=AAA68404.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076940(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076940 NCBI Caenorhabditis elegans C14F5.4 (C14F5.4) mRNA, complete cds. 11/11 None
SNAP00000042706 ENSEMBL cdna:SNAP chromosome:CEL140:X:7956317:7958239:-1 11/11 None
GENEFINDER00000042711 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7956317:7958239:-1 11/11 None
C14F5.4 ENSEMBL cdna:known chromosome:CEL140:X:7956161:7958245:-1 gene:C14F5.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7956610-7959499(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C14F5.4
Entrez Gene 181054 Entrez gene
SwissProt Q09972 EMBL-EBI
WormBase CE30370 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.16037.1.S1_AT similar to Sideroflexin-2 cfa
DROSOPHILA_2:1631456_AT dm
DROSGENOME1:149029_AT dm
CHICKEN:GGA.12531.1.S1_AT sideroflexin 2 gga
HU35KSUBC:RC_AA496127_AT sideroflexin 2 hs
U133_X3P:HS.44070.0.A1_3P_AT sideroflexin 2 hs
HG-U133_PLUS_2:227560_AT sideroflexin 2 hs
HG-U133B:227560_AT sideroflexin 2 hs
HG-U95B:45596_AT sideroflexin 2 hs
MG-U74AV2:103706_AT sideroflexin 2 mm
MOE430A:1435896_AT sideroflexin 2 mm
MOUSE430A_2:1435896_AT sideroflexin 2 mm
MOUSE430_2:1435896_AT sideroflexin 2 mm
MU11KSUBA:AA189555_S_AT sideroflexin 2 mm
MG-U74AV2:160967_AT sideroflexin 2 mm
MG-U74CV2:166322_R_AT sideroflexin 2 mm
MOE430A:1449853_AT sideroflexin 2 mm
MOUSE430A_2:1449853_AT sideroflexin 2 mm
MOUSE430_2:1449853_AT sideroflexin 2 mm
MOE430B:1439966_X_AT Sideroflexin 2, mRNA (cDNA clone MGC:30418 IMAGE:5043343) mm
MOUSE430_2:1439966_X_AT Sideroflexin 2, mRNA (cDNA clone MGC:30418 IMAGE:5043343) mm
MOE430B:1440786_X_AT Sideroflexin 2, mRNA (cDNA clone MGC:30418 IMAGE:5043343) mm
MOUSE430_2:1440786_X_AT Sideroflexin 2, mRNA (cDNA clone MGC:30418 IMAGE:5043343) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6812 cation transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8324 cation transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68404 Hypothetical protein C14F5.4 [Caenorhabditis elegans] ref|NP_509341.2| C14F5.4 [Caenorhabditis elegans] 0.0
blast CAE70326 Hypothetical protein CBG16855 [Caenorhabditis briggsae] 1.0E-175
blast AAT27471 mitochondrial sideroflexin 2 [Steinernema carpocapsae] 1.0E-106
Protein Domains
Database ID Description E-Value
Pfam IPR004686 EMBL-EBI Tricarboxylate/iron carrier 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509341.2 4 90-112,141-163,224-246,261-283
C14F5.4 4 90-112,141-163,224-246,261-283
SNAP00000042706 4 90-112,141-163,224-246,261-283
GENEFINDER00000042711 4 90-112,141-163,224-246,261-283
Sequence
Target Sequence 
>C. ELEGANS:181067_AT
gccagtgccggcaacacttgaggaacttcatcgttcgcaacgtctgtgtcaatcagcttt
tcatccggatactggagaacttcagaactttgctggaaggatgtgcttcaacgtctgggg
tggtacaatgctgtgtggtgcaatgatgatctggtacaaatcgacgccagctgtaatttt
ctggcaatgggcaaatcagtcatttaatgcactggtcaattataccaatcgaaatgcaaa
gagcacattgacaacaaaagacttagttgtatcgtattctacagcagtgtctggagcact
ggcaatggcaattgggctgaaaacatattttgctaagaaacagagcagtcctttggctca
acgattagtaccactgggagccgttgcagtggctaatgctattaacatccctatgatgcg
tcagaatgaactcaaagaaggtatgactgtcactgatgccgatggaaacaacgttggagt
ttcccggttagccgcagctaaagccatctccctggtggttctctccagaaatattatcgt
tgccccatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCAGTGCCGGCAACACTTGAGGAA 358 281 267 Antisense
TCGTTCGCAACGTCTGTGTCAATCA 103 605 297 Antisense
GTCAATCAGCTTTTCATCCGGATAC 442 463 314 Antisense
TGCTTCAACGTCTGGGGTGGTACAA 683 441 370 Antisense
AAATCGACGCCAGCTGTAATTTTCT 140 119 424 Antisense
GCAGTCCTTTGGCTCAACGATTAGT 427 291 611 Antisense
GATTAGTACCACTGGGAGCCGTTGC 560 413 629 Antisense
GACTGTCACTGATGCCGATGGAAAC 44 377 711 Antisense
GTTAGCCGCAGCTAAAGCCATCTCC 210 445 753 Antisense
TCCCTGGTGGTTCTCTCCAGAAATA 423 597 775 Antisense
CAGAAATATTATCGTTGCCCCATGC 640 203 792 Antisense
Sequence Source Affymetrix Proprietary Database