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[181065_at]

Description ZK563.4 /REP_DB=WormBase Gene ID /WP=CE05095 /TR=Q23532 /GB=AAA81150.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 11      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_highest_nonorm > 71      6grp_total > 32      4grp_total > 44      6grp_highest_sub > De      6grp_cgc > 60      6grp_high1_sub > De      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 278
1 2 3 4 range
A 455.5
(1.6)
P 		519.8
(3)
P 		297.9
(-1.8)
P 		241.9
(-3)
P 		278
B 384.2
(1.5)
P 		420.3
(3)
P 		279.1
(-2.9)
P 		275.9
(-3)
P 		144
C 416.8
(3)
P 		414.2
(2.9)
P 		296.3
(-2.3)
P 		280.9
(-3)
P 		136
avr 418.8
(1.9) 		451.4
(3) 		291.1
(-2.2) 		266.2
(-3) 		185

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 273
1 2 3 4 5 6 range
A 468.6
(3)
P 		336
(-0.1)
P 		397.4
(1.4)
P 		346.9
(0.2)
P 		270.6
(-1.6)
P 		209.6
(-3)
P 		259
B 401.8
(3)
P 		318.2
(0.6)
P 		357.2
(1.7)
P 		357.2
(1.7)
P 		316.5
(0.5)
P 		195.4
(-3)
P 		206
C 298.6
(-0.6)
P 		395.2
(3)
P 		338.9
(0.9)
P 		314.1
(0)
P 		260.5
(-2)
P 		232.6
(-3)
P 		163
avr 389.7
(3) 		349.8
(1.6) 		364.5
(2.1) 		339.4
(1.3) 		282.5
(-0.6) 		212.5
(-3) 		177
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 165.8
(3)
P 		112.8
(0.6)
P 		77.9
(-1)
P 		35.4
(-3)
P 		130
B 122.5
(3)
P 		103.8
(1.2)
P 		64.7
(-2.4)
P 		58.8
(-3)
P 		64
C 138
(3)
P 		119.1
(1.7)
P 		103.3
(0.7)
P 		47.4
(-3)
P 		91
avr 142.1
(3) 		111.9
(1.1) 		82
(-0.8) 		47.2
(-3) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 89
1 2 3 4 5 6 range
A 86.4
(0.1)
P 		119.5
(3)
P 		86.5
(0.1)
P 		88
(0.2)
P 		81.6
(-0.3)
P 		51
(-3)
P 		69
B 139.8
(3)
P 		105.9
(-0)
P 		86.7
(-1.8)
P 		94.6
(-1.1)
P 		128.6
(2)
P 		72.9
(-3)
P 		67
C 110.7
(2)
P 		120.6
(3)
P 		86.3
(-0.6)
P 		63.2
(-3)
P 		84.3
(-0.8)
P 		77.9
(-1.5)
P 		57
avr 112.3
(2.6) 		115.3
(3) 		86.5
(-0.6) 		81.9
(-1.2) 		98.2
(0.9) 		67.3
(-3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 181065_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18463
Sequence Type Exemplar sequence
Representative Public ID ZK563.4 NCBI
Target Description ZK563.4 /REP_DB=WormBase Gene ID /WP=CE05095 /TR=Q23532 /GB=AAA81150.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029823(11), NM_001029822(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029823 NCBI Caenorhabditis elegans CLaudin-like in Caenorhabditis family member (clc-3) (clc-3) mRNA, complete cds. 11/11 None
NM_001029822 NCBI Caenorhabditis elegans CLaudin-like in Caenorhabditis family member (clc-3) (clc-3) mRNA, complete cds. 11/11 None
SNAP00000043683 ENSEMBL cdna:SNAP chromosome:CEL140:X:3373304:3374656:-1 11/11 None
GENEFINDER00000043689 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3373304:3374656:-1 11/11 None
ZK563.4b ENSEMBL cdna:known chromosome:CEL140:X:3373235:3374728:-1 gene:ZK563.4 11/11 None
ZK563.4a ENSEMBL cdna:known chromosome:CEL140:X:3373241:3374656:-1 gene:ZK563.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3373304-3374657(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZK563.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81150 Claudin-like in caenorhabditis protein 3, isoform a [Caenorhabditis elegans] ref|NP_001024993.1| CLaudin-like in Caenorhabditis family member (clc-3) [Caenorhabditis elegans] 1.0E-129
blast CAE68417 Hypothetical protein CBG14196 [Caenorhabditis briggsae] 1.0E-122
blast AAO91697 Claudin-like in caenorhabditis protein 3, isoform b [Caenorhabditis elegans] ref|NP_001024994.1| CLaudin-like in Caenorhabditis family member (clc-3) [Caenorhabditis elegans] 1.0E-118
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024994.1 4 12-34,98-120,133-152,180-202
NP_001024993.1 4 12-34,98-120,133-152,180-202
ZK563.4b 4 12-34,98-120,133-152,180-202
ZK563.4a 4 12-34,98-120,133-152,180-202
SNAP00000043683 4 12-34,98-120,133-152,180-202
GENEFINDER00000043689 4 12-34,98-120,133-152,180-202
Sequence
Target Sequence 
>C. ELEGANS:181065_AT
ggcactggaatgctggtcatcgcgttgatctccgactattggtccattcatcaacctcgc
aaccaaatggacaatcttcaaatgcaccgcggagtcctgcgtcaatgcatcactacgcga
caatatggcagttgtaatttccgtctgtcaagcatgttcaaacagctgcgcaatttcatg
gatggatacgacatgtactcggaaagatccatgtatcgacacttacctacacaaacatat
gaagtctttgtcgcgctctttcttgccatttcctgtatgattgcatctaccgtactccta
tttggtccgttttgctgccaacgttgcaagccgtccactacgctgcttatttttatcact
ggagtgttctctggatctggatgtttaatctattggaatgcaaatcgcgaaggcaaaacg
ttcacgttacagcaactccaattccaggatgtataccattatgactacggaagtgacatg
aatatcttatcttggtcgttttggatggctgtcgtcagcaccggcatacttctgtgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCACTGGAATGCTGGTCATCGCGT 523 535 67 Antisense
TCATCGCGTTGATCTCCGACTATTG 697 621 83 Antisense
CTATTGGTCCATTCATCAACCTCGC 255 223 102 Antisense
AATCTTCAAATGCACCGCGGAGTCC 274 167 139 Antisense
AATTTCCGTCTGTCAAGCATGTTCA 68 175 202 Antisense
GATCCATGTATCGACACTTACCTAC 257 415 272 Antisense
TCTTTCTTGCCATTTCCTGTATGAT 640 615 323 Antisense
GTCCACTACGCTGCTTATTTTTATC 487 475 399 Antisense
GGAGTGTTCTCTGGATCTGGATGTT 479 517 427 Antisense
AACGTTCACGTTACAGCAACTCCAA 326 145 483 Antisense
TCAGCACCGGCATACTTCTGTGCTC 40 625 581 Antisense
Sequence Source Affymetrix Proprietary Database