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[181053_s_at]

Description T19E7.3 /REP_DB=WormBase Gene ID /WP=CE27592 /TR=Q22592 /GB=AAA83597.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 84      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 64      6grp_total > 75      4grp_total > 01      6grp_cgc > 67      6grp_cgc_sub > In      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 677
1 2 3 4 range
A 473
(-3)
P 		606.4
(-1.6)
P 		540.4
(-2.3)
P 		1049.1
(3)
P 		576
B 551.6
(-2.9)
P 		540.2
(-3)
P 		647
(-1.9)
P 		1150.3
(3)
P 		610
C 649.7
(-1.8)
P 		530.6
(-3)
P 		664.3
(-1.7)
P 		1149.6
(3)
P 		619
avr 558.1
(-3) 		559.1
(-3) 		617.2
(-2.4) 		1116.3
(3) 		558

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 642
1 2 3 4 5 6 range
A 541.7
(-2.9)
P 		530.2
(-3)
P 		687.6
(-1.1)
P 		1029.3
(3)
P 		737.9
(-0.5)
P 		962.9
(2.2)
P 		499
B 555.6
(-2)
P 		483.8
(-3)
P 		655.4
(-0.5)
P 		817.4
(1.8)
P 		729.2
(0.5)
P 		903.9
(3)
P 		420
C 657.7
(-2.3)
P 		595.5
(-3)
P 		818.9
(-0.5)
P 		1088.6
(2.6)
P 		730.7
(-1.5)
P 		1125.8
(3)
P 		530
avr 585
(-2.4) 		536.5
(-3) 		720.6
(-0.6) 		978.4
(2.8) 		732.6
(-0.4) 		997.5
(3) 		461
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 172.2
(3)
P 		131.6
(-3)
P 		141.3
(-1.6)
P 		153.6
(0.3)
P 		41
B 175.9
(-0.7)
P 		133.4
(-3)
P 		149.9
(-2.1)
P 		245.1
(3)
P 		112
C 215.1
(1.8)
P 		152.6
(-3)
P 		231.5
(3)
P 		193.8
(0.1)
P 		79
avr 187.7
(2) 		139.2
(-3) 		174.2
(0.6) 		197.5
(3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 277
1 2 3 4 5 6 range
A 99.9
(-3)
P 		188.6
(0.3)
P 		149.7
(-1.1)
P 		261.1
(3)
P 		222.7
(1.6)
P 		234.4
(2)
P 		161
B 193.3
(-1.8)
P 		161
(-2.9)
P 		159.2
(-3)
P 		216.5
(-1.1)
P 		296.3
(1.6)
P 		337.1
(3)
P 		178
C 243.8
(-1.1)
P 		181.7
(-3)
P 		208.4
(-2.2)
P 		218.9
(-1.9)
P 		236.4
(-1.3)
P 		376.9
(3)
P 		195
avr 179
(-2.7) 		177.1
(-2.8) 		172.4
(-3) 		232.2
(-0.5) 		251.8
(0.3) 		316.1
(3) 		144
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181053_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11064
Sequence Type Exemplar sequence
Representative Public ID T19E7.3 NCBI
Target Description T19E7.3 /REP_DB=WormBase Gene ID /WP=CE27592 /TR=Q22592 /GB=AAA83597.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068443(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068443 NCBI Caenorhabditis elegans BEClin (human autophagy) homolog family member (bec-1) (bec-1) mRNA, complete cds. 11/11 None
SNAP00000009629 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5661325:5664402:-1 11/11 None
GENEFINDER00000009634 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5661325:5664402:-1 11/11 None
T19E7.3.1 ENSEMBL cdna:known chromosome:CEL140:IV:5661189:5664410:-1 gene:T19E7.3 11/11 None
T19E7.3.2 ENSEMBL cdna:known chromosome:CEL140:IV:5661190:5664412:-1 gene:T19E7.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5661330-5663754(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol bec-1
Ensembl T19E7.3
Entrez Gene 177345 Entrez gene
SwissProt Q22592 EMBL-EBI
WormBase CE27592 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251286_AT autophagy protein Apg6 family at
CHICKEN:GGA.9029.1.S1_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) gga
CHICKEN:GGAAFFX.12111.1.S1_S_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) gga
HG-U133A:208946_S_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
HG-U133_PLUS_2:208946_S_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
HG-U133A_2:208946_S_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
HG-FOCUS:208946_S_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
HUGENEFL:L38932_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
U133_X3P:G4680380_3P_A_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
U133_X3P:HS.12272.0.S1_3P_A_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
HG-U133A_2:208945_S_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
HG-U133_PLUS_2:208945_S_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
HG-U133A:208945_S_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
HG-U95AV2:39378_AT beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
U133_X3P:HS2.372893.1.S1_3P_AT Beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
HG-U133_PLUS_2:1561652_AT Beclin 1 (coiled-coil, myosin-like BCL2 interacting protein) hs
MOUSE430_2:1430358_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOE430B:1430358_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MU11KSUBA:AA177231_S_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MG-U74AV2:160380_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MG-U74CV2:170217_R_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOE430A:1460320_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOUSE430A_2:1460320_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOUSE430_2:1460320_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOE430A:1435219_X_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOUSE430A_2:1435219_X_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOUSE430_2:1435219_X_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOE430A:1437006_X_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOUSE430A_2:1437006_X_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOUSE430_2:1437006_X_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOE430A:1455880_S_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOUSE430A_2:1455880_S_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MOUSE430_2:1455880_S_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MG-U74BV2:163076_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) mm
MG-U74BV2:164507_F_AT Beclin 1 (coiled-coil, myosin-like BCL2-interacting protein), mRNA (cDNA clone MGC:6843 IMAGE:2650059) mm
MU19KSUBA:TC16005_AT Beclin 1 (coiled-coil, myosin-like BCL2-interacting protein), mRNA (cDNA clone MGC:6843 IMAGE:2650059) mm
MU19KSUBA:TC16005_G_AT Beclin 1 (coiled-coil, myosin-like BCL2-interacting protein), mRNA (cDNA clone MGC:6843 IMAGE:2650059) mm
MU19KSUBA:TC16817_AT Beclin 1 (coiled-coil, myosin-like BCL2-interacting protein), mRNA (cDNA clone MGC:6843 IMAGE:2650059) mm
RG-U34A:RC_AA891035_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) rn
RAE230A:1367457_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) rn
RAE230B:1367457_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) rn
RAT230_2:1367457_AT beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) rn
RG-U34B:RC_AA957071_AT Beclin 1 (coiled-coil, myosin-like BCL2-interacting protein) rn
YEAST_2:1779594_AT Protein that forms a membrane-associated complex with Apg14p that is essential for autophagy; involved in a retrieval step of the carboxypeptidase Y receptor, Vps10p, to the late Golgi from the endosome; involved in vacuolar protein sorting Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6914 autophagy inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83597 Beclin (human autophagy) homolog protein 1 [Caenorhabditis elegans] ref|NP_500844.1| BEClin (human autophagy) homolog family member (bec-1) [Caenorhabditis elegans] 1.0E-178
blast CAE72670 Hypothetical protein CBG19886 [Caenorhabditis briggsae] 1.0E-158
Protein Domains
Database ID Description E-Value
Pfam IPR007243 EMBL-EBI Autophagy protein Apg6 1.9E-30
Sequence
Target Sequence 
>C. ELEGANS:181053_S_AT
atcaggacgagcttcattcgctggaagccgaacgtcaatatgcagaagttcagcatagaa
aactaacagacacaaatgttctcgacttgtgcttccacatttgggttgatggaattgttg
gtgaaatcaatggatttcggttgggatatttaaaagatgctccagtagaatttacagaga
tcaatgcagctctaggacaaattgttttgttattagagatcctgttggagcgtatcggcg
tacaacatcacgaactgatgcccgtcgccatgggtagtcactcgtacattaagcttcgtc
gtaatggcatcgacatggaaacgtacgcattgtatggacaaggaactccactatcaggat
catctggaattgatcctggaattcgtcgattcttgcagttgctcgagtttttgttgaaag
agctcaaggatcgaaacaaaaatttcaaaccaccatatcaaattcatgctgattcactgg
ttgacaatggtgtcaaatacaatgcagttatgacactcaacacagatgttcgatggactc
gtgcaatggctctaatgctcacagatctcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAGGACGAGCTTCATTCGCTGGA 154 27 380 Antisense
CAAATGTTCTCGACTTGTGCTTCCA 426 193 452 Antisense
GGAGCGTATCGGCGTACAACATCAC 453 519 606 Antisense
AACATCACGAACTGATGCCCGTCGC 116 135 623 Antisense
GTCACTCGTACATTAAGCTTCGTCG 508 463 656 Antisense
GGAACTCCACTATCAGGATCATCTG 588 529 721 Antisense
AATTCGTCGATTCTTGCAGTTGCTC 490 177 759 Antisense
GCAGTTGCTCGAGTTTTTGTTGAAA 672 311 774 Antisense
AATTCATGCTGATTCACTGGTTGAC 606 175 840 Antisense
GATGGACTCGTGCAATGGCTCTAAT 173 407 911 Antisense
TGGCTCTAATGCTCACAGATCTCAA 495 543 926 Antisense
Sequence Source Affymetrix Proprietary Database