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[181046_s_at]

Description T08A9.9 /REP_DB=WormBase Gene ID /WP=CE04924 /TR=Q22339 /GB=AAA81417.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 08      6grp_high1_10_10 > 80      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 18      6grp_total > 71      4grp_total > 59      6grp_cgc > 75      6grp_high1_sub > 02_03_04_05      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18712
1 2 3 4 range
A 20352.6
(0.9)
P 		25944.9
(2.8)
P 		26651.7
(3)
P 		8473.6
(-3)
P 		18178
B 20830.5
(-0.6)
P 		23815.9
(1.1)
P 		27185.3
(3)
P 		16521.8
(-3)
P 		10664
C 20195.2
(0.8)
P 		20018.5
(0.6)
P 		22428.6
(3)
P 		16308.3
(-3)
P 		6120
avr 20459.4
(0.4) 		23259.8
(1.9) 		25421.9
(3) 		13767.9
(-3) 		11654

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23423
1 2 3 4 5 6 range
A 7944.9
(-3)
P 		14653.4
(-0.2)
P 		22407.8
(3)
P 		21784.4
(2.7)
P 		18326.7
(1.3)
P 		16159
(0.4)
P 		14463
B 16852.9
(-2.4)
P 		20592
(-0.2)
P 		26206.5
(3)
P 		20473.5
(-0.3)
P 		17827.3
(-1.8)
P 		15744.8
(-3)
P 		10462
C 17057.8
(1.5)
P 		21757.8
(3)
P 		2783.2
(-3)
P 		21456.7
(2.9)
P 		17571.7
(1.7)
P 		14136.6
(0.6)
P 		18975
avr 13951.9
(-3) 		19001.1
(1.2) 		17132.5
(-0.4) 		21238.2
(3) 		17908.6
(0.3) 		15346.8
(-1.9) 		7286
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6577
1 2 3 4 range
A 7410.4
(3)
P 		5631.9
(1.3)
P 		6967.3
(2.6)
P 		1240.4
(-3)
P 		6170
B 6642.7
(3)
P 		5882
(1.5)
P 		6299.5
(2.3)
P 		3520.8
(-3)
P 		3122
C 6685.2
(1.7)
P 		5755.5
(0.6)
P 		7817.1
(3)
P 		2749.8
(-3)
P 		5067
avr 6912.8
(2.8) 		5756.5
(1.3) 		7028
(3) 		2503.7
(-3) 		4524

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6535
1 2 3 4 5 6 range
A 1464.6
(-3)
P 		5211.5
(2.5)
P 		4880
(2)
P 		5525
(3)
P 		5530.2
(3)
P 		3934.2
(0.6)
P 		4066
B 5864
(-1.5)
P 		6854.2
(1.7)
P 		6363.7
(0.1)
P 		5422.7
(-3)
P 		7243
(3)
P 		5871.2
(-1.5)
P 		1820
C 6322.6
(2.7)
P 		6639
(3)
P 		708.4
(-3)
P 		4315
(0.6)
P 		5685.9
(2)
P 		4732.3
(1.1)
P 		5931
avr 4550.4
(-1.5) 		6234.9
(3) 		3984
(-3) 		5087.6
(-0.1) 		6153
(2.8) 		4845.9
(-0.7) 		2251
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 181046_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16765
Sequence Type Exemplar sequence
Representative Public ID T08A9.9 NCBI
Target Description T08A9.9 /REP_DB=WormBase Gene ID /WP=CE04924 /TR=Q22339 /GB=AAA81417.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029725(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029725 NCBI Caenorhabditis elegans SaPosin-like Protein family member (spp-5) (spp-5) mRNA, complete cds. 11/11 None
T08A9.9a ENSEMBL cdna:known chromosome:CEL140:X:7319939:7322353:-1 gene:T08A9.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029726 6/11 Cross Hyb Matching Probes None
SNAP00000001755 6/11 Cross Hyb Matching Probes None
GENEFINDER00000001766 6/11 Cross Hyb Matching Probes None
SNAP00000001756 5/11 Cross Hyb Matching Probes None
GENEFINDER00000001769 5/11 Cross Hyb Matching Probes None
T08A9.9b.2 6/11 Cross Hyb Matching Probes None
T08A9.9b.1 6/11 Cross Hyb Matching Probes None
T08A9.9b.3 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180994 2 1 NM_001029725 181046_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7319937-7322352(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spp-5
Ensembl T08A9.9
Entrez Gene 180994 Entrez gene
SwissProt Q86FL8 EMBL-EBI
WormBase CE33825 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001024896 SaPosin-like Protein family member (spp-5) [Caenorhabditis elegans] pir||E89567 protein T08A9.9 [imported] - Caenorhabditis elegans 2.0E-99
blast NP_001024897 SaPosin-like Protein family member (spp-5) [Caenorhabditis elegans] gb|AAP13768.1| Saposin-like protein family protein 5 [Caenorhabditis elegans] 4.0E-48
blast AAA81417 Hypothetical protein T08A9.13 [Caenorhabditis elegans] 1.0E-42
Protein Domains
Database ID Description E-Value
Pfam IPR008138 EMBL-EBI Saposin-like type B, 2 2.0E-10
Sequence
Target Sequence 
>C. ELEGANS:181046_S_AT
tcagcactgcaccaattccagtagacaatattaccgacccacatgccagacgaacagcag
aactcagaaacgcagaactggaaaagcttcaaaaagcgaaggagctcgagcaacgaacta
gggttgaatgtcaacaggctcttgaccaatacgatgtactgaaaaaaatcccaacattga
cggaacaagaacggaaagaaaacgagacgctcaccaaaaagaaaatagctaaagccatta
ttgctgcacaaaaagcttctcaagctgtgatggatgcacgtgtaattatgggagccaaag
gcgaggaactgacaaaaatgaagactcttcttgtactagccgttttggtcgccgttgcct
caggacttgccatcccagaaaatagaagcgcattgagctgtcaaatgtgtgagttggttg
tcaagaaatacgaaggatccgccgataaggatgccaatgtcatcaagaaggatttcgatg
ctgaatgcaagaagcttttccacaccatcccattcggaaccagagaatgcgatcattacg
tcaacagcaaggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAGCACTGCACCAATTCCAGTAGA 498 619 56 Antisense
GTAGACAATATTACCGACCCACATG 194 457 76 Antisense
ACCCACATGCCAGACGAACAGCAGA 223 91 92 Antisense
AACAGGCTCTTGACCAATACGATGT 370 137 188 Antisense
AGCTTCTCAAGCTGTGATGGATGCA 287 79 309 Antisense
TCTTGTACTAGCCGTTTTGGTCGCC 68 617 384 Antisense
TCAGGACTTGCCATCCCAGAAAATA 483 621 415 Antisense
GGATCCGCCGATAAGGATGCCAATG 193 513 490 Antisense
ATGCAAGAAGCTTTTCCACACCATC 524 43 540 Antisense
CACCATCCCATTCGGAACCAGAGAA 652 201 558 Antisense
GCGATCATTACGTCAACAGCAAGGT 17 291 584 Antisense
Sequence Source Affymetrix Proprietary Database