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[180999_at]

Description T20B5.2 /REP_DB=WormBase Gene ID /WP=CE02880 /TR=Q22600 /GB=AAA68331.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 15      4grp_highest_8_8 > 50      4grp_high_8_8 > 33      6grp_highest_nonorm > 80      6grp_total > 15      4grp_total > 47      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 439
1 2 3 4 range
A 552.1
(3)
P 		254
(-1.1)
P 		163.7
(-2.4)
P 		119.7
(-3)
P 		432
B 558.5
(3)
P 		191
(-2.5)
P 		197.8
(-2.4)
P 		158.1
(-3)
P 		400
C 487.7
(3)
P 		216
(-1.7)
P 		142.7
(-3)
P 		196.2
(-2.1)
P 		345
avr 532.8
(3) 		220.3
(-2) 		168.1
(-2.8) 		158
(-3) 		375

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 279
1 2 3 4 5 6 range
A 345
(3)
P 		302.9
(1.9)
P 		151.8
(-2)
P 		160.9
(-1.8)
P 		147.1
(-2.1)
P 		113.3
(-3)
P 		232
B 305.2
(3)
P 		293.8
(2.6)
P 		211.6
(-0.4)
P 		154.5
(-2.5)
P 		155
(-2.5)
P 		139.9
(-3)
P 		165
C 392.3
(3)
P 		321.2
(1.4)
P 		237.2
(-0.5)
P 		153.7
(-2.4)
P 		134.9
(-2.8)
P 		125.1
(-3)
P 		267
avr 347.5
(3) 		306
(1.9) 		200.2
(-1) 		156.4
(-2.2) 		145.7
(-2.5) 		126.1
(-3) 		221
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 201
(3)
P 		55.1
(-1.8)
P 		42.8
(-2.2)
P 		17.5
(-3)
P 		184
B 178.1
(3)
P 		47.2
(-2.4)
P 		45.8
(-2.5)
P 		33.7
(-3)
P 		144
C 161.4
(3)
P 		62.1
(-1.6)
P 		49.7
(-2.2)
P 		33.1
(-3)
P 		128
avr 180.2
(3) 		54.8
(-1.9) 		46.1
(-2.3) 		28.1
(-3) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 118
1 2 3 4 5 6 range
A 63.6
(-0.3)
P 		107.7
(3)
P 		33.1
(-2.6)
P 		40.8
(-2)
P 		44.4
(-1.7)
P 		27.6
(-3)
P 		80
B 106.2
(3)
P 		97.8
(2.2)
P 		51.4
(-2)
P 		40.9
(-3)
P 		63
(-1)
P 		52.2
(-2)
P 		65
C 145.4
(3)
P 		98
(0.5)
P 		60.4
(-1.5)
P 		30.9
(-3)
P 		43.6
(-2.3)
P 		41.9
(-2.4)
P 		115
avr 105.1
(3) 		101.2
(2.7) 		48.3
(-2) 		37.5
(-3) 		50.3
(-1.9) 		40.6
(-2.7) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180999_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16681
Sequence Type Exemplar sequence
Representative Public ID T20B5.2 NCBI
Target Description T20B5.2 /REP_DB=WormBase Gene ID /WP=CE02880 /TR=Q22600 /GB=AAA68331.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077170(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077170 NCBI Caenorhabditis elegans T20B5.2 (T20B5.2) mRNA, complete cds. 10/11 None
GENEFINDER00000028855 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9331170:9336323:1 10/11 None
T20B5.2 ENSEMBL cdna:known chromosome:CEL140:X:9331075:9336497:1 gene:T20B5.2 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9331169-9336323(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20B5.2
Entrez Gene 181162 Entrez gene
SwissProt Q22600 EMBL-EBI
WormBase CE28681 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68331 Hypothetical protein T20B5.2 [Caenorhabditis elegans] ref|NP_509571.1| T20B5.2 [Caenorhabditis elegans] 1.0E-109
blast CAE69835 Hypothetical protein CBG16159 [Caenorhabditis briggsae] 1.0E-102
Sequence
Target Sequence 
>C. ELEGANS:180999_AT
aactagcgttattcagcagccgtctgagccagtccgtccagcatcaccagtccgacctgt
cactccagttggtggagaaaatgagttgagccggcacttgctccaacctatccaacctct
ccatatcccacgcatcgacattcagcctgccaattcttcacgacacgcatcgccaagaca
taaattgcagtatgatgaggtttcaccaagatcaggttctatcaacatgctatcgccaag
atctggacactcggcatcttcaagatctggagctggatctagacagagtaatgtgtccgt
aaattcggcaaatgtgccattgaaaaaggtcaccaagaagtcccgatttgtttccattca
cgatggtcgtcccgtatccccatacacagaaacagtgtcttttgaagttgccattccatt
tcaaccagatagatattccagcgcttcggcccgttcattctccagctcaccacacgtttc
ccgcaggccaagcgaatgcgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTAGCGTTATTCAGCAGCCGTCT 426 139 288 Antisense
AATGAGTTGAGCCGGCACTTGCTCC 293 163 367 Antisense
ATCGACATTCAGCCTGCCAATTCTT 211 37 421 Antisense
GGTTCTATCAACATGCTATCGCCAA 324 507 502 Antisense
GCTATCGCCAAGATCTGGACACTCG 17 305 516 Antisense
GACACTCGGCATCTTCAAGATCTGG 510 367 533 Antisense
CAAGAAGTCCCGATTTGTTTCCATT 564 183 621 Antisense
TGTTTCCATTCACGATGGTCGTCCC 373 559 636 Antisense
TGCCATTCCATTTCAACCAGATAGA 482 585 696 Antisense
CAGATAGATATTCCAGCGCTTCGGC 191 207 713 Antisense
TTCCCGCAGGCCAAGCGAATGCGCA 630 695 765 Antisense
Sequence Source Affymetrix Proprietary Database