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[180983_at]

Description F49C12.4 /REP_DB=WormBase Gene ID /WP=CE03364 /TR=Q20582 /GB=CAA92509.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 10      4grp_total > 16      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 13.6
(-3)
A 		29.9
(-2)
A 		47.4
(-0.9)
A 		108.5
(3)
A 		95
B 42.5
(-3)
A 		42.8
(-2.9)
A 		43.8
(-2.5)
A 		56.8
(3)
A 		14
C 25.6
(-3)
A 		40.3
(-1.7)
A 		40.8
(-1.6)
A 		92.3
(3)
A 		67
avr 27.2
(-3) 		37.7
(-1.9) 		44
(-1.3) 		85.9
(3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1027
1 2 3 4 5 6 range
A 66
(-3)
A 		843.9
(3)
P 		790.7
(2.6)
P 		542.7
(0.7)
P 		500.9
(0.4)
P 		205.6
(-1.9)
P 		778
B 32.8
(-3)
A 		1044.6
(3)
P 		831.3
(1.7)
P 		682.8
(0.9)
P 		688.3
(0.9)
P 		310.8
(-1.4)
P 		1012
C 17.3
(-3)
A 		977.3
(3)
P 		485.5
(-0.1)
P 		715.6
(1.4)
P 		444.4
(-0.3)
P 		336.9
(-1)
P 		960
avr 38.7
(-3) 		955.3
(3) 		702.5
(1.3) 		647
(1) 		544.5
(0.3) 		284.4
(-1.4) 		917
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 5
(-3)
A 		6.5
(-2.2)
A 		12.4
(1.1)
A 		15.9
(3)
A 		11
B 13.6
(3)
A 		10.6
(-2.1)
A 		10.1
(-3)
A 		12.1
(0.4)
A 		4
C 8.5
(-3)
A 		11.6
(-0.4)
A 		14.2
(1.8)
A 		15.6
(3)
A 		7
avr 9
(-3) 		9.6
(-2.3) 		12.2
(0.5) 		14.5
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 341
1 2 3 4 5 6 range
A 12.2
(-3)
A 		300.1
(3)
P 		172.2
(0.3)
P 		137.6
(-0.4)
P 		151.2
(-0.1)
P 		50.1
(-2.2)
P 		288
B 11.4
(-3)
A 		347.7
(3)
P 		201.9
(0.4)
P 		180.8
(0)
P 		279.7
(1.8)
P 		115.9
(-1.1)
P 		336
C 6.4
(-3)
A 		298.2
(3)
P 		123.6
(-0.6)
P 		143.9
(-0.2)
P 		143.8
(-0.2)
P 		112.8
(-0.8)
P 		292
avr 10
(-3) 		315.3
(3) 		165.9
(0.1) 		154.1
(-0.2) 		191.6
(0.6) 		92.9
(-1.4) 		305
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180983_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9295
Sequence Type Exemplar sequence
Representative Public ID F49C12.4 NCBI
Target Description F49C12.4 /REP_DB=WormBase Gene ID /WP=CE03364 /TR=Q20582 /GB=CAA92509.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069225(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069225 NCBI Caenorhabditis elegans F49C12.4 (F49C12.4) mRNA, complete cds. 11/11 None
SNAP00000032692 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9302642:9304071:-1 11/11 None
GENEFINDER00000032715 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9302642:9304071:-1 11/11 None
F49C12.4 ENSEMBL cdna:known chromosome:CEL140:IV:9302642:9304071:-1 gene:F49C12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9302647-9304077(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49C12.4
Entrez Gene 186027 Entrez gene
SwissProt Q20582 EMBL-EBI
WormBase CE03364 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92509 Hypothetical protein F49C12.4 [Caenorhabditis elegans] ref|NP_501626.1| F49C12.4 [Caenorhabditis elegans] 1.0E-179
blast CAA92507 Hypothetical protein F49C12.2 [Caenorhabditis elegans] ref|NP_501624.1| F49C12.2 [Caenorhabditis elegans] 1.0E-121
blast CAA99867 Hypothetical protein K01D12.1 [Caenorhabditis elegans] ref|NP_506099.1| K01D12.1 [Caenorhabditis elegans] 3.0E-98
Protein Domains
Database ID Description E-Value
Pfam IPR002485 EMBL-EBI Protein of unknown function DUF13 9.1E-60
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501626.1 1 20-39
F49C12.4 1 20-39
SNAP00000032692 1 20-39
GENEFINDER00000032715 1 20-39
Sequence
Target Sequence 
>C. ELEGANS:180983_AT
ggtgacattgggtattggccacgatgttagtggagaaatcactctgaaacagatttggcc
aaaaacagagtttttcggagccgatccaagttcagatatcaacaaggacttgtatgagaa
agaactcggaggaaaatatttccagtatgctgttggaggtcaaaatgggatgcaagaagc
ttttgtgctaggcaaaaacgaaggagactaccgtaccaagaccgtgatgcacattggagc
agaaattttttttagagacgaaataaagaaacaaaaaatcgatatattatggatggacat
tgaaggacacgagtttccagttatggatatgcttcatcgagatggcccgtttgataaaaa
aggtgttaaaatatgccagatcaacgttgaaattcataaagatattgacaatgggatcac
tggagaaagacaaatgttccatgattttgtttggaaattaatgggcgatggccgatatgt
aatggtcaaaccattttctgtttggttctaccatgattttattcgcactgtgcttattaa
tgtttacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTGACATTGGGTATTGGCCACGAT 413 501 402 Antisense
TTTCGGAGCCGATCCAAGTTCAGAT 50 669 474 Antisense
ATTTCCAGTATGCTGTTGGAGGTCA 209 17 539 Antisense
AGACTACCGTACCAAGACCGTGATG 26 71 606 Antisense
AGACCGTGATGCACATTGGAGCAGA 198 71 620 Antisense
CTTCATCGAGATGGCCCGTTTGATA 462 219 733 Antisense
AAAATATGCCAGATCAACGTTGAAA 519 133 769 Antisense
GGGCGATGGCCGATATGTAATGGTC 352 493 864 Antisense
CCATTTTCTGTTTGGTTCTACCATG 182 271 892 Antisense
ACCATGATTTTATTCGCACTGTGCT 201 87 911 Antisense
GCACTGTGCTTATTAATGTTTACGA 321 315 926 Antisense
Sequence Source Affymetrix Proprietary Database