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[180973_at]

Description F49F1.6 /REP_DB=WormBase Gene ID /WP=CE17089 /TR=Q9GZE3 /GB=AAF99963.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 51      6grp_total > 07      4grp_total > 61      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 487
1 2 3 4 range
A 635.6
(3)
P 		614.9
(2.7)
P 		627.5
(2.9)
P 		212.4
(-3)
A 		423
B 691.7
(3)
P 		587.8
(1.6)
P 		541
(1)
P 		248.8
(-3)
P 		443
C 649.3
(3)
P 		597.3
(2.3)
P 		577.5
(2)
P 		204.4
(-3)
A 		445
avr 658.9
(3) 		600
(2.2) 		582
(1.9) 		221.9
(-3) 		437

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 397
1 2 3 4 5 6 range
A 439.4
(3)
P 		265.1
(0.1)
P 		159.5
(-1.7)
P 		217.8
(-0.7)
P 		79.3
(-3)
A 		167.9
(-1.5)
A 		360
B 407.1
(3)
P 		281
(0.6)
P 		218.9
(-0.5)
P 		186.7
(-1.2)
P 		89
(-3)
A 		122
(-2.4)
A 		318
C 475.9
(3)
P 		242.9
(-1)
P 		307.9
(0.1)
P 		127.9
(-3)
A 		127.7
(-3)
A 		130.1
(-3)
A 		348
avr 440.8
(3) 		263
(-0.1) 		228.8
(-0.7) 		177.5
(-1.6) 		98.7
(-3) 		140
(-2.3) 		342
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 200
1 2 3 4 range
A 231.4
(3)
P 		133.5
(0.1)
P 		164.1
(1)
P 		31.1
(-3)
A 		200
B 220.6
(3)
P 		145.2
(0.3)
P 		125.4
(-0.4)
P 		53
(-3)
P 		168
C 214.9
(3)
P 		171.7
(1.6)
P 		201.3
(2.5)
P 		34.5
(-3)
A 		180
avr 222.3
(3) 		150.1
(0.6) 		163.6
(1.1) 		39.5
(-3) 		183

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 81
(1.9)
P 		94.3
(3)
P 		34.7
(-2.1)
P 		55.2
(-0.3)
P 		23.9
(-3)
A 		40.9
(-1.6)
A 		70
B 141.6
(3)
P 		93.5
(0.3)
P 		53.1
(-2)
P 		49.5
(-2.2)
P 		36.2
(-3)
A 		45.5
(-2.5)
A 		105
C 176.4
(3)
P 		74.1
(-1.1)
P 		78.4
(-0.9)
P 		25.7
(-3)
A 		41.3
(-2.4)
A 		43.6
(-2.3)
A 		151
avr 133
(3) 		87.3
(0.2) 		55.4
(-1.7) 		43.5
(-2.4) 		33.8
(-3) 		43.3
(-2.4) 		99
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180973_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11246
Sequence Type Exemplar sequence
Representative Public ID F49F1.6 NCBI
Target Description F49F1.6 /REP_DB=WormBase Gene ID /WP=CE17089 /TR=Q9GZE3 /GB=AAF99963.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068086(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068086 NCBI Caenorhabditis elegans F49F1.6 (F49F1.6) mRNA, complete cds. 11/11 None
SNAP00000034584 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4121374:4123013:-1 11/11 None
GENEFINDER00000034603 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4119494:4123013:-1 11/11 None
F49F1.6 ENSEMBL cdna:known chromosome:CEL140:IV:4121364:4123013:-1 gene:F49F1.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4121369-4123019(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49F1.6
Entrez Gene 186063 Entrez gene
SwissProt Q9GZE3 EMBL-EBI
WormBase CE34873 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF99963 Hypothetical protein F49F1.6 [Caenorhabditis elegans] ref|NP_500487.2| F49F1.6 [Caenorhabditis elegans] 1.0E-131
blast AAF99963 Hypothetical protein F49F1.6 [Caenorhabditis elegans] ref|NP_500487.2| F49F1.6 [Caenorhabditis elegans] 1.0E-128
blast AAF99963 Hypothetical protein F49F1.6 [Caenorhabditis elegans] ref|NP_500487.2| F49F1.6 [Caenorhabditis elegans] 7.0E-93
blast AAF99969 Hypothetical protein F49F1.5 [Caenorhabditis elegans] ref|NP_500486.1| F49F1.5 [Caenorhabditis elegans] 5.0E-85
blast AAF99968 Hypothetical protein F49F1.1 [Caenorhabditis elegans] ref|NP_500485.1| F49F1.1 [Caenorhabditis elegans] 7.0E-58
blast AAF99968 Hypothetical protein F49F1.1 [Caenorhabditis elegans] ref|NP_500485.1| F49F1.1 [Caenorhabditis elegans] 1.0E-54
blast AAF99961 Hypothetical protein F49F1.7 [Caenorhabditis elegans] ref|NP_500488.1| F49F1.7 [Caenorhabditis elegans] 3.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 6.9E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.8E-7
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.3E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.6E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.6E-14
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 0.99
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.8E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.3E-6
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.5E-7
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.5E-11
Sequence
Target Sequence 
>C. ELEGANS:180973_AT
gcagagtcaatgcccatacacttgtggaatatgccatcaggatccaaatcaatgtactga
cactaacttagattgcttgaagtttgattcaatgtgcaagacggatgtgaagtatcagaa
acaatgcccatacacctgtggaacctgcgtaccagctaccccggacagctgctttgatca
tatgattgagtgcccaaattatagcgttccatgtactgacgctgttaagatattctgccc
acggacctgtggagtttgtggaagtactggggtacctatggctcaaactacgttgctcac
tacggtaaagcctacgactacagtagtaccaagtactacaacaaaactgccagctaccac
gaccaagaagcttccatcaactacgccaaagccaccatgcaaggattcgtcgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAGAGTCAATGCCCATACACTTGT 165 311 132 Antisense
CAATGCCCATACACCTGTGGAACCT 22 183 253 Antisense
CCCCGGACAGCTGCTTTGATCATAT 182 261 290 Antisense
GTGCCCAAATTATAGCGTTCCATGT 418 477 321 Antisense
GTTCCATGTACTGACGCTGTTAAGA 271 439 337 Antisense
TATTCTGCCCACGGACCTGTGGAGT 663 663 362 Antisense
GTACTGGGGTACCTATGGCTCAAAC 709 457 395 Antisense
TATGGCTCAAACTACGTTGCTCACT 413 653 408 Antisense
GTTGCTCACTACGGTAAAGCCTACG 260 439 423 Antisense
CAAGAAGCTTCCATCAACTACGCCA 570 185 495 Antisense
AAGCCACCATGCAAGGATTCGTCGC 399 149 520 Antisense
Sequence Source Affymetrix Proprietary Database