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[180966_s_at]

Description W04B5.1 /REP_DB=WormBase Gene ID /WP=CE20138 /TR=Q9UA59 /GB=AAC68914.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 48      6grp_high1_10_10 > 84      4grp_highest_8_8 > 15      4grp_high_8_8 > 06      6grp_highest_nonorm > 72      6grp_total > 74      4grp_total > 02      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1266
1 2 3 4 range
A 248.2
(-3)
P 		365.8
(-2.4)
P 		270.2
(-2.9)
P 		1513.9
(3)
P 		1266
B 299
(-2.6)
P 		258.4
(-3)
P 		363.1
(-2.1)
P 		949.9
(3)
P 		692
C 260.2
(-3)
P 		338.7
(-2.5)
P 		257
(-3)
P 		1304.3
(3)
P 		1047
avr 269.1
(-3) 		321
(-2.7) 		296.8
(-2.8) 		1256
(3) 		987

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 344
1 2 3 4 5 6 range
A 352.8
(-1.4)
P 		276.8
(-2.7)
P 		531.6
(1.7)
P 		603.3
(3)
P 		259.5
(-3)
P 		460.7
(0.5)
P 		344
B 294.6
(-3)
P 		414.5
(0.9)
P 		390.8
(0.1)
P 		468.3
(2.7)
P 		478.8
(3)
P 		447.4
(2)
P 		184
C 274.8
(-3)
P 		420.7
(1.8)
P 		310.2
(-1.8)
P 		322
(-1.4)
P 		414.4
(1.6)
P 		457.4
(3)
P 		183
avr 307.4
(-3) 		370.7
(-0.6) 		410.9
(1) 		464.5
(3) 		384.2
(-0.1) 		455.2
(2.6) 		157
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 90.4
(-2.2)
P 		79.4
(-2.7)
P 		70.6
(-3)
P 		221.6
(3)
P 		151
B 95.4
(-1.6)
P 		63.8
(-3)
P 		84.1
(-2.1)
P 		202.4
(3)
P 		139
C 86.1
(-3)
P 		97.4
(-2.5)
P 		89.6
(-2.8)
P 		219.9
(3)
P 		134
avr 90.6
(-2.5) 		80.2
(-3) 		81.4
(-2.9) 		214.6
(3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 65
(-3)
P 		98.4
(-0.7)
P 		115.8
(0.5)
P 		153
(3)
P 		78.3
(-2.1)
P 		112.2
(0.2)
P 		88
B 102.5
(-2.5)
P 		138
(-0.4)
P 		94.9
(-3)
P 		124
(-1.2)
P 		194.5
(3)
P 		166.8
(1.3)
P 		100
C 101.9
(-0.5)
P 		128.4
(1.3)
P 		78.9
(-2)
P 		64.8
(-3)
P 		134.1
(1.7)
P 		153.1
(3)
P 		88
avr 89.8
(-3) 		121.6
(0.5) 		96.5
(-2.3) 		113.9
(-0.3) 		135.6
(2.1) 		144
(3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180966_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6913
Sequence Type Exemplar sequence
Representative Public ID W04B5.1 NCBI
Target Description W04B5.1 /REP_DB=WormBase Gene ID /WP=CE20138 /TR=Q9UA59 /GB=AAC68914.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065153(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065153 NCBI Caenorhabditis elegans W04B5.1 (W04B5.1) mRNA, complete cds. 11/11 None
SNAP00000019374 ENSEMBL cdna:SNAP chromosome:CEL140:III:2428509:2429344:1 11/11 None
W04B5.1 ENSEMBL cdna:known chromosome:CEL140:III:2428212:2429351:1 gene:W04B5.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_075861 3/11 Cross Hyb Matching Probes A 
GENEFINDER00000019377 8/11 Cross Hyb Matching Probes None
SNAP00000013814 5/11 Cross Hyb Matching Probes None
GENEFINDER00000026605 6/11 Cross Hyb Matching Probes None
SNAP00000026596 6/11 Cross Hyb Matching Probes None
ZK402.3 3/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2428508-2429344(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W04B5.1
Entrez Gene 175362 Entrez gene
SwissProt Q9UA59 EMBL-EBI
WormBase CE28846 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast ABA00182 Hypothetical protein ZK380.5 [Caenorhabditis elegans] 2.0E-49
blast AAC68914 Hypothetical protein W04B5.1 [Caenorhabditis elegans] ref|NP_497554.1| W04B5.1 [Caenorhabditis elegans] 8.0E-28
blast AAC68914 Hypothetical protein W04B5.1 [Caenorhabditis elegans] ref|NP_497554.1| W04B5.1 [Caenorhabditis elegans] 4.0E-21
Sequence
Target Sequence 
>C. ELEGANS:180966_S_AT
gaactggtggcgagcttgaggggatggcgcgacggaattcgtcgtgacatagaatggagc
agaagattcatagatcgtctgcgacacgcacgcggcgatctcctcaacaaaacgcgtcag
gagaatagagaattacgcaaaataattctcaatctcgaggccctggatgagattgaagga
ctcattagagacagaataaatgcttctggtctccttgccattcaagtcaccattcaagtg
ccacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACTGGTGGCGAGCTTGAGGGGAT 123 349 175 Antisense
TGAGGGGATGGCGCGACGGAATTCG 216 489 191 Antisense
GGCGCGACGGAATTCGTCGTGACAT 47 543 200 Antisense
TTCATAGATCGTCTGCGACACGCAC 17 689 241 Antisense
GATCTCCTCAACAAAACGCGTCAGG 204 419 271 Antisense
TACGCAAAATAATTCTCAATCTCGA 314 649 308 Antisense
TTCTCAATCTCGAGGCCCTGGATGA 706 691 320 Antisense
GGCCCTGGATGAGATTGAAGGACTC 34 543 333 Antisense
GAATAAATGCTTCTGGTCTCCTTGC 159 327 368 Antisense
TCTCCTTGCCATTCAAGTCACCATT 53 611 384 Antisense
TCAAGTCACCATTCAAGTGCCACCA 129 633 396 Antisense
Sequence Source Affymetrix Proprietary Database