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[180944_s_at]

Description F14B8.6 /REP_DB=WormBase Gene ID /WP=CE02649 /TR=Q19446 /GB=AAA68276.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 70      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 64      6grp_total > 90      4grp_total > 31      6grp_highest_sub > 04_05      6grp_high1_sub > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 448
1 2 3 4 range
A 1164.3
(3)
P 		716.2
(-3)
P 		762.2
(-2.4)
P 		727.6
(-2.8)
P 		448
B 1134.8
(3)
P 		762.5
(-3)
P 		787.9
(-2.6)
P 		1111.9
(2.6)
P 		372
C 1113.8
(3)
P 		836.4
(-1.9)
P 		772
(-3)
P 		997.8
(1)
P 		342
avr 1137.6
(3) 		771.7
(-3) 		774
(-3) 		945.8
(-0.1) 		366

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 424
1 2 3 4 5 6 range
A 602.5
(-3)
P 		719.8
(-1.3)
P 		688.4
(-1.8)
P 		1026.5
(3)
P 		709.7
(-1.5)
P 		805.5
(-0.1)
P 		424
B 806.1
(0.7)
P 		791.8
(0.4)
P 		692.7
(-1.5)
P 		920.7
(3)
P 		771
(0)
P 		619.5
(-3)
P 		301
C 926
(3)
P 		777.3
(-1)
P 		764.7
(-1.3)
P 		895.8
(2.2)
P 		792.5
(-0.6)
P 		702.8
(-3)
P 		223
avr 778.2
(-1.3) 		763
(-1.6) 		715.3
(-2.8) 		947.7
(3) 		757.7
(-1.8) 		709.3
(-3) 		238
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 317
1 2 3 4 range
A 423.9
(3)
P 		155.5
(-2.1)
P 		199.3
(-1.2)
P 		106.5
(-3)
P 		317
B 361.9
(3)
P 		188.3
(-2.8)
P 		182.6
(-3)
P 		236.9
(-1.2)
P 		179
C 368.7
(3)
P 		240.5
(-0.8)
P 		269
(0)
P 		168.3
(-3)
P 		200
avr 384.8
(3) 		194.8
(-2.3) 		217
(-1.7) 		170.6
(-3) 		214

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 232
1 2 3 4 5 6 range
A 111.1
(-3)
P 		256
(2.8)
P 		149.9
(-1.4)
P 		260.3
(3)
P 		214.1
(1.1)
P 		196.1
(0.4)
P 		149
B 280.5
(1.6)
P 		263.6
(0.9)
P 		168.2
(-3)
P 		243.9
(0.1)
P 		313.3
(3)
P 		231
(-0.4)
P 		145
C 343.2
(3)
P 		237.2
(-0.9)
P 		194.6
(-2.5)
P 		180.1
(-3)
P 		256.5
(-0.2)
P 		235.3
(-1)
P 		163
avr 244.9
(1.9) 		252.3
(2.4) 		170.9
(-3) 		228.1
(0.8) 		261.3
(3) 		220.8
(0.3) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180944_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16146
Sequence Type Exemplar sequence
Representative Public ID F14B8.6 NCBI
Target Description F14B8.6 /REP_DB=WormBase Gene ID /WP=CE02649 /TR=Q19446 /GB=AAA68276.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076772(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076772 NCBI Caenorhabditis elegans F14B8.6 (F14B8.6) mRNA, complete cds. 11/11 None
SNAP00000027543 ENSEMBL cdna:SNAP chromosome:CEL140:X:6929425:6932021:-1 11/11 None
GENEFINDER00000027544 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6929425:6931270:-1 11/11 None
F14B8.6 ENSEMBL cdna:known chromosome:CEL140:X:6929205:6932309:-1 gene:F14B8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6929424-6932021(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F14B8.6
Entrez Gene 180966 Entrez gene
SwissProt Q19446 EMBL-EBI
WormBase CE02649 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68276 Hypothetical protein F14B8.6 [Caenorhabditis elegans] ref|NP_509173.1| F14B8.6 [Caenorhabditis elegans] 0.0
blast CAE65821 Hypothetical protein CBG10935 [Caenorhabditis briggsae] 1.0E-134
blast CAE65821 Hypothetical protein CBG10935 [Caenorhabditis briggsae] 1.0E-130
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509173.1 1 225-247
F14B8.6 1 225-247
SNAP00000027543 1 225-247
GENEFINDER00000027544 1 207-229
Sequence
Target Sequence 
>C. ELEGANS:180944_S_AT
accaatggactcttactgcgacaataaatcgaaagtgcaatggagcggaccagtacgggt
tcaattgtaatgaacaatgctcgacggttaataacgattattattgctacacgtgtggca
gtaatgggcaaaaaacatgctgtgcgagtggggatgttaacccagacgattgttcttatt
atgatcacccgatctccacaacatggtcacctaatacacaatgttcagcatccgctgaaa
acacgtacttctggcttatgatcagttttgctatcatcatcgccattctcgccattctcc
ttctcctcgtcctgcttgaactttgctgtggattattcacagggcgtcagtcggcaagaa
gaagcgaagatggtgattggatcgtaccaaaaacgccaaaagcgaatcacgagctttacg
acgccgacataacacctcatcatcaatatcggcgtagacatcaggataataattcagggg
aaagcacagagccagaagatagaagatctccgtatattgtcagtag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCAATGGACTCTTACTGCGACAAT 283 83 437 Antisense
GGAGCGGACCAGTACGGGTTCAATT 529 517 478 Antisense
GTTAACCCAGACGATTGTTCTTATT 665 445 592 Antisense
TATTATGATCACCCGATCTCCACAA 215 663 613 Antisense
GATCTCCACAACATGGTCACCTAAT 108 419 627 Antisense
AACACGTACTTCTGGCTTATGATCA 155 137 676 Antisense
AGTTTTGCTATCATCATCGCCATTC 667 63 700 Antisense
TCGTCCTGCTTGAACTTTGCTGTGG 336 605 743 Antisense
GCGAATCACGAGCTTTACGACGCCG 71 287 838 Antisense
ACACCTCATCATCAATATCGGCGTA 272 105 868 Antisense
GAAGATCTCCGTATATTGTCAGTAG 32 339 938 Antisense
Sequence Source Affymetrix Proprietary Database