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[180930_s_at]

Description F25E2.2 /REP_DB=WormBase Gene ID /WP=CE07131 /TR=Q19780 /GB=AAA91255.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 83      4grp_total > 40      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 61.4
(-3)
A 		97.7
(0.9)
A 		114.2
(2.7)
A 		117.2
(3)
P 		56
B 76.5
(-1.4)
A 		98.3
(0.5)
A 		126.6
(3)
P 		58.9
(-3)
A 		68
C 70.8
(-3)
A 		85.4
(-0.7)
A 		109.7
(3)
M 		92.6
(0.4)
A 		39
avr 69.6
(-3) 		93.8
(0.1) 		116.8
(3) 		89.6
(-0.5) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 174
1 2 3 4 5 6 range
A 91.5
(-2.2)
A 		77
(-3)
A 		118.4
(-0.6)
P 		182.7
(3)
P 		121.6
(-0.5)
P 		119.9
(-0.6)
P 		106
B 32.9
(-3)
A 		86.3
(-0.8)
P 		101.5
(-0.2)
P 		161.4
(2.3)
P 		178.5
(3)
P 		85.5
(-0.8)
P 		146
C 52.1
(-3)
A 		108.5
(-0.8)
P 		125.4
(-0.2)
P 		206.9
(3)
P 		186.6
(2.2)
P 		117.3
(-0.5)
P 		155
avr 58.8
(-3) 		90.6
(-1.5) 		115.1
(-0.3) 		183.7
(3) 		162.2
(2) 		107.6
(-0.7) 		125
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 22.4
(-0.5)
A 		21.2
(-1.1)
A 		29.9
(3)
A 		17.2
(-3)
P 		13
B 24.4
(1.2)
A 		24.3
(1.2)
A 		29.3
(3)
P 		12.6
(-3)
A 		17
C 23.4
(-0.9)
A 		24.6
(-0.6)
A 		38.2
(3)
M 		15.6
(-3)
A 		23
avr 23.4
(-0.1) 		23.4
(-0.1) 		32.5
(3) 		15.1
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 16.9
(-3)
A 		27.4
(-0.9)
A 		25.8
(-1.2)
P 		46.3
(3)
P 		36.7
(1)
P 		29.2
(-0.5)
P 		29
B 11.4
(-3)
A 		28.7
(-1.3)
P 		24.7
(-1.7)
P 		42.8
(0.1)
P 		72.5
(3)
P 		31.9
(-1)
P 		61
C 19.3
(-3)
A 		33.1
(-1)
P 		31.9
(-1.2)
P 		41.6
(0.3)
P 		60.4
(3)
P 		39.3
(-0.1)
P 		41
avr 15.9
(-3) 		29.7
(-1) 		27.5
(-1.3) 		43.6
(1.1) 		56.5
(3) 		33.5
(-0.4) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180930_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18549
Sequence Type Exemplar sequence
Representative Public ID F25E2.2 NCBI
Target Description F25E2.2 /REP_DB=WormBase Gene ID /WP=CE07131 /TR=Q19780 /GB=AAA91255.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075757(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075757 NCBI Caenorhabditis elegans F25E2.2 (F25E2.2) mRNA, complete cds. 11/11 None
GENEFINDER00000017582 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:806613:809991:-1 11/11 None
SNAP00000017574 ENSEMBL cdna:SNAP chromosome:CEL140:X:807083:809991:-1 11/11 None
F25E2.2 ENSEMBL cdna:known chromosome:CEL140:X:806994:810739:-1 gene:F25E2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:807082-810722(-) 99.17 100.0
Public Domain and Genome References
Gene Title
Ensembl F25E2.2
Entrez Gene 180428 Entrez gene
SwissProt Q19780 EMBL-EBI
WormBase CE37106 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA91255 Hypothetical protein F25E2.2 [Caenorhabditis elegans] ref|NP_508158.2| F25E2.2 [Caenorhabditis elegans] 0.0
blast CAE63618 Hypothetical protein CBG08111 [Caenorhabditis briggsae] 1.0E-121
blast CAE63618 Hypothetical protein CBG08111 [Caenorhabditis briggsae] 1.0E-120
blast CAE63618 Hypothetical protein CBG08111 [Caenorhabditis briggsae] 1.0E-118
blast CAE56357 Hypothetical protein CBG24031 [Caenorhabditis briggsae] 1.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.4E-4
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.4E-4
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.4E-4
Sequence
Target Sequence 
>C. ELEGANS:180930_S_AT
gttaagaatctattgccatacggaggcagaaaatgcaaagtttttgaaagaagcagtgaa
gaagttctcgacgaatccgaaaaatagatcgagaatgttggaattcatagatcatgtttt
cttggaagaacaagaacctgcacaccagaaagaacaaaacaaagaacaggattgcccaat
aaactcgaaatcttgtgaaaagtgctctcactccaagtgtgaaaaatgtgcaaaacaccc
tcatcaccattgtaaatgtgaagatcagaaagagtttaatagaaaaattgtgagagcgtt
gtgcccaaaaacgtgcgatatttgcaacgaaacacatccatcggtttcaacgattttgtc
aactagaatttctgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTAAGAATCTATTGCCATACGGAG 652 445 1545 Antisense
GAAGAAGTTCTCGACGAATCCGAAA 55 341 1602 Antisense
GAACAAGAACCTGCACACCAGAAAG 313 353 1672 Antisense
GAACAGGATTGCCCAATAAACTCGA 459 351 1708 Antisense
GAAAAGTGCTCTCACTCCAAGTGTG 89 353 1741 Antisense
AAATGTGCAAAACACCCTCATCACC 56 121 1768 Antisense
AACACCCTCATCACCATTGTAAATG 295 127 1778 Antisense
GAAAAATTGTGAGAGCGTTGTGCCC 261 355 1826 Antisense
GAGCGTTGTGCCCAAAAACGTGCGA 360 387 1838 Antisense
AAACGTGCGATATTTGCAACGAAAC 507 127 1853 Antisense
GATTTTGTCAACTAGAATTTCTGGA 690 429 1896 Antisense
Sequence Source Affymetrix Proprietary Database