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[180925_at]

Description F49F1.1 /REP_DB=WormBase Gene ID /WP=CE17084 /TR=Q9GZE5 /GB=AAF99968.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 47      6grp_total > 56      4grp_total > 57      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 271
1 2 3 4 range
A 209.6
(-2.1)
P 		272.2
(-0.6)
P 		425.4
(3)
P 		170.9
(-3)
A 		255
B 206.2
(-1.4)
P 		344.3
(3)
P 		331.7
(2.6)
P 		154.4
(-3)
A 		190
C 234.8
(-3)
P 		339.7
(1.8)
P 		366.2
(3)
P 		273.7
(-1.2)
P 		131
avr 216.9
(-2.4) 		318.7
(1.1) 		374.4
(3) 		199.7
(-3) 		175

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 136
1 2 3 4 5 6 range
A 120.9
(0.5)
A 		120.2
(0.4)
A 		148.2
(3)
M 		94.6
(-1.9)
A 		83.2
(-3)
A 		145.8
(2.8)
A 		65
B 139
(1.3)
A 		123
(-0.3)
A 		94.1
(-3)
A 		136.1
(1)
A 		142.4
(1.6)
A 		157.3
(3)
A 		63
C 146.8
(0)
M 		182
(1.6)
M 		154.6
(0.4)
A 		206.1
(2.6)
A 		78
(-3)
A 		214.1
(3)
M 		136
avr 135.6
(-0.1) 		141.7
(0.4) 		132.3
(-0.4) 		145.6
(0.7) 		101.2
(-3) 		172.4
(3) 		71
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 76.3
(0.6)
P 		59.1
(-0.6)
P 		111.2
(3)
P 		25
(-3)
A 		86
B 65.7
(0.8)
P 		85
(3)
P 		76.9
(2.1)
P 		32.9
(-3)
A 		52
C 77.7
(-0.7)
P 		97.7
(0.8)
P 		127.6
(3)
P 		46.2
(-3)
P 		81
avr 73.2
(0.3) 		80.6
(0.9) 		105.2
(3) 		34.7
(-3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 49
1 2 3 4 5 6 range
A 22.3
(-3)
A 		42.7
(3)
A 		32.3
(-0.1)
M 		24
(-2.5)
A 		25.1
(-2.2)
A 		35.5
(0.9)
A 		20
B 48.4
(1.3)
A 		40.9
(0)
A 		22.8
(-3)
A 		36
(-0.8)
A 		57.8
(2.9)
A 		58.6
(3)
A 		36
C 54.4
(0.8)
M 		55.5
(0.9)
M 		39.3
(-1.2)
A 		41.4
(-0.9)
A 		25.2
(-3)
A 		71.7
(3)
M 		47
avr 41.7
(-0.4) 		46.4
(0.8) 		31.5
(-3) 		33.8
(-2.4) 		36
(-1.9) 		55.3
(3) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180925_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10904
Sequence Type Exemplar sequence
Representative Public ID F49F1.1 NCBI
Target Description F49F1.1 /REP_DB=WormBase Gene ID /WP=CE17084 /TR=Q9GZE5 /GB=AAF99968.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068084(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068084 NCBI Caenorhabditis elegans F49F1.1 (F49F1.1) mRNA, complete cds. 11/11 None
SNAP00000034583 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4117796:4118733:1 11/11 None
GENEFINDER00000034600 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4115936:4119077:1 11/11 None
F49F1.1 ENSEMBL cdna:known chromosome:CEL140:IV:4117796:4119077:1 gene:F49F1.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4117801-4119083(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49F1.1
Entrez Gene 177176 Entrez gene
SwissProt Q9GZE5 EMBL-EBI
WormBase CE17084 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF99968 Hypothetical protein F49F1.1 [Caenorhabditis elegans] ref|NP_500485.1| F49F1.1 [Caenorhabditis elegans] 1.0E-119
blast AAF99968 Hypothetical protein F49F1.1 [Caenorhabditis elegans] ref|NP_500485.1| F49F1.1 [Caenorhabditis elegans] 3.0E-97
blast AAF99968 Hypothetical protein F49F1.1 [Caenorhabditis elegans] ref|NP_500485.1| F49F1.1 [Caenorhabditis elegans] 6.0E-95
blast AAF99963 Hypothetical protein F49F1.6 [Caenorhabditis elegans] ref|NP_500487.2| F49F1.6 [Caenorhabditis elegans] 6.0E-55
blast AAF99963 Hypothetical protein F49F1.6 [Caenorhabditis elegans] ref|NP_500487.2| F49F1.6 [Caenorhabditis elegans] 2.0E-47
blast AAF99963 Hypothetical protein F49F1.6 [Caenorhabditis elegans] ref|NP_500487.2| F49F1.6 [Caenorhabditis elegans] 3.0E-35
blast CAE56271 Hypothetical protein CBG23916 [Caenorhabditis briggsae] 3.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.3E-9
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.2E-7
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.8E-7
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.2E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.8E-13
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 0.05
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.2E-7
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.8E-7
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.2E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.8E-13
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000034600 2 2-21,26-48
Sequence
Target Sequence 
>C. ELEGANS:180925_AT
tgtaattcgccctcatttcaacgacagtgtgccggaacctgcggactttgcccaggagcc
tgtgaggacatatgggatggatgttcccattttttggcgccttgttcagatgacattaag
agcctctgcccaattaaatgtggactttgcaatagcactactactccaatgatcctaact
accataactcctgtagacccccgatgtgtggacgctggaaatcagtgcgccaattattca
ccgccttgctcggatgatgtgaagatattctgcccggccacttgtggaagttgctctaat
ggcacaactggtgtgcctctggttcttactaccttgactccagaacttactacagtgaca
gttgagccgattacgtctacattgaagccaacaactactaccataacaccaactacaaca
actaaactaccaactaccacaaccaaaaagcttccaccaactacaccaaagccgccgtgc
aaggatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTAATTCGCCCTCATTTCAACGAC 689 557 217 Antisense
TCAACGACAGTGTGCCGGAACCTGC 376 631 234 Antisense
GACTTTGCCCAGGAGCCTGTGAGGA 645 371 260 Antisense
CCATTTTTTGGCGCCTTGTTCAGAT 627 267 303 Antisense
TAAGAGCCTCTGCCCAATTAAATGT 336 637 333 Antisense
GCACTACTACTCCAATGATCCTAAC 471 315 371 Antisense
TGATCCTAACTACCATAACTCCTGT 190 565 386 Antisense
TCAGTGCGCCAATTATTCACCGCCT 450 623 438 Antisense
TACTACCTTGACTCCAGAACTTACT 583 645 543 Antisense
GAGCCGATTACGTCTACATTGAAGC 698 383 580 Antisense
ACACCAAAGCCGCCGTGCAAGGATT 571 107 679 Antisense
Sequence Source Affymetrix Proprietary Database