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[180918_at]

Description F22E5.2 /REP_DB=WormBase Gene ID /WP=CE09554 /TR=O16716 /GB=AAB66168.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 92      4grp_total > 12      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 24.9
(1)
A 		5.9
(-3)
A 		7.1
(-2.7)
A 		34.5
(3)
A 		29
B 3.8
(-2.3)
A 		4.7
(-1.2)
A 		3.2
(-3)
A 		8.2
(3)
A 		5
C 2.9
(-3)
A 		7.9
(-2)
A 		3.3
(-2.9)
A 		31.8
(3)
A 		29
avr 10.5
(-1.2) 		6.2
(-2.5) 		4.5
(-3) 		24.8
(3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 4.6
(-2.8)
A 		3.1
(-3)
A 		3.7
(-2.9)
A 		5.1
(-2.7)
A 		39.5
(3)
A 		5.2
(-2.7)
A 		36
B 5.2
(-3)
A 		9.7
(-2.2)
A 		5.6
(-2.9)
A 		18.5
(-0.7)
A 		40.3
(3)
A 		5.4
(-3)
A 		35
C 3.8
(-3)
A 		38.5
(0.4)
A 		4
(-3)
A 		65.2
(3)
A 		30.6
(-0.4)
A 		14.2
(-2)
A 		61
avr 4.5
(-3) 		17.1
(-0.6) 		4.4
(-3) 		29.6
(1.7) 		36.8
(3) 		8.3
(-2.3) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 9.1
(3)
A 		1.3
(-3)
A 		1.9
(-2.5)
A 		5.1
(-0.1)
A 		8
B 1.2
(0)
A 		1.2
(0)
A 		0.7
(-3)
A 		1.7
(3)
A 		1
C 0.9
(-3)
A 		2.3
(-1.1)
A 		1.1
(-2.7)
A 		5.4
(3)
A 		5
avr 3.7
(2.2) 		1.6
(-2.2) 		1.2
(-3) 		4.1
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 0.8
(-3)
A 		1.1
(-2.8)
A 		0.8
(-3)
A 		1.3
(-2.7)
A 		11.9
(3)
A 		1.3
(-2.7)
A 		11
B 1.8
(-2.8)
A 		3.2
(-2.3)
A 		1.4
(-3)
A 		4.9
(-1.6)
A 		16.4
(3)
A 		2
(-2.8)
A 		15
C 1.4
(-2.8)
A 		11.7
(2.3)
A 		1
(-3)
A 		13.1
(3)
A 		9.9
(1.4)
A 		4.7
(-1.2)
A 		12
avr 1.3
(-2.9) 		5.3
(-0.8) 		1.1
(-3) 		6.4
(-0.3) 		12.7
(3) 		2.7
(-2.2) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180918_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4240
Sequence Type Exemplar sequence
Representative Public ID F22E5.2 NCBI
Target Description F22E5.2 /REP_DB=WormBase Gene ID /WP=CE09554 /TR=O16716 /GB=AAB66168.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061924(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061924 NCBI Caenorhabditis elegans F22E5.2 (F22E5.2) mRNA, complete cds. 11/11 None
GENEFINDER00000023623 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2665390:2666170:1 9/11 None
F22E5.2 ENSEMBL cdna:known chromosome:CEL140:II:2665390:2666253:1 gene:F22E5.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027253 3/11 Cross Hyb Matching Probes None
SNAP00000006772 3/11 Cross Hyb Matching Probes None
GENEFINDER00000006780 4/11 Cross Hyb Matching Probes None
SNAP00000023616 4/11 Cross Hyb Matching Probes None
Y27F2A.9 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2665388-2666252(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F22E5.2
Entrez Gene 184832 Entrez gene
SwissProt O16716 EMBL-EBI
WormBase CE09554 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494325 F22E5.2 [Caenorhabditis elegans] gb|AAB66168.1| Hypothetical protein F22E5.2 [Caenorhabditis elegans] 7.0E-81
blast NP_494325 F22E5.2 [Caenorhabditis elegans] gb|AAB66168.1| Hypothetical protein F22E5.2 [Caenorhabditis elegans] 2.0E-47
blast NP_001022424 Y27F2A.9 [Caenorhabditis elegans] gb|AAT92060.1| Hypothetical protein Y27F2A.9 [Caenorhabditis elegans] 2.0E-28
blast NP_001022424 Y27F2A.9 [Caenorhabditis elegans] gb|AAT92060.1| Hypothetical protein Y27F2A.9 [Caenorhabditis elegans] 1.0E-16
Sequence
Target Sequence 
>C. ELEGANS:180918_AT
attcttctactttatggttctgtcagaagcctgtatgagaactgtcgcgccggaagatgt
gtacatttccagtacatttccttttgaagattttacaacagaagcagttgtcacaactga
ttcatcgactatttcacaactaaccacagagacgactatggaaacagtggaaacccacat
tttttatgttacatgcaagaacagatatccgatttctaatcgtaattgtttgattaatcg
acatacagatgattttttctgctttatccttccagtagatgcttgtatgagaactgtccc
accggaagacgtgtatatttccattacattgccttacgaagactttaccacagaaagtaa
tgaaattgccgtcacaactgattcatcgactgttccccaactagtcacagaaaccactga
aataccaactgcaactaccacgccgggatggagctcaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCTTCTACTTTATGGTTCTGTCA 368 11 27 Antisense
AAGCCTGTATGAGAACTGTCGCGCC 623 147 53 Antisense
GTCGCGCCGGAAGATGTGTACATTT 611 473 70 Antisense
ACATTTCCAGTACATTTCCTTTTGA 538 103 89 Antisense
CATCGACTATTTCACAACTAACCAC 193 211 149 Antisense
ATGATTTTTTCTGCTTTATCCTTCC 312 47 275 Antisense
TGCTTGTATGAGAACTGTCCCACCG 304 579 306 Antisense
ATATTTCCATTACATTGCCTTACGA 224 19 341 Antisense
ATTGCCGTCACAACTGATTCATCGA 143 7 391 Antisense
TGTTCCCCAACTAGTCACAGAAACC 375 561 417 Antisense
TACCACGCCGGGATGGAGCTCAAAA 368 649 462 Antisense
Sequence Source Affymetrix Proprietary Database