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[180913_at]

Description F32A5.5 /REP_DB=WormBase Gene ID /WP=CE01275 /TR=Q19949 /GB=AAC46664.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 42      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 26      6grp_total > 52      4grp_total > 54      6grp_highest_sub > 03      6grp_cgc > 81      6grp_high1_sub > 02_03      6grp_cgc_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2515
1 2 3 4 range
A 4216.1
(3)
P 		2916.2
(-0.1)
P 		2868.7
(-0.2)
P 		1700.8
(-3)
P 		2515
B 4155.6
(3)
P 		3058.4
(0.1)
P 		2754.5
(-0.7)
P 		1891.7
(-3)
P 		2264
C 4141
(3)
P 		3055
(0.3)
P 		2765.8
(-0.4)
P 		1745.5
(-3)
P 		2396
avr 4170.9
(3) 		3009.9
(0.1) 		2796.3
(-0.4) 		1779.3
(-3) 		2392

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3984
1 2 3 4 5 6 range
A 4984.6
(0.2)
P 		5428
(1.2)
P 		6255.9
(3)
P 		5223.3
(0.7)
P 		4933
(0.1)
P 		3519.3
(-3)
P 		2737
B 4430
(-1)
P 		5342.4
(0.6)
P 		6764.4
(3)
P 		4588.6
(-0.7)
P 		5510.1
(0.9)
P 		3224.7
(-3)
P 		3540
C 4142.6
(-1.9)
P 		5433.5
(0.2)
P 		7208.6
(3)
P 		4947.2
(-0.6)
P 		5100.9
(-0.4)
P 		3443.1
(-3)
P 		3766
avr 4519.1
(-1) 		5401.3
(0.6) 		6743
(3) 		4919.7
(-0.3) 		5181.3
(0.2) 		3395.7
(-3) 		3347
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1286
1 2 3 4 range
A 1535.1
(3)
P 		633
(-1.2)
P 		749.9
(-0.7)
P 		249
(-3)
P 		1286
B 1325.2
(3)
P 		755.4
(-0.7)
P 		638.3
(-1.5)
P 		403.1
(-3)
P 		922
C 1370.8
(3)
P 		878.3
(0.3)
P 		964
(0.7)
P 		294.3
(-3)
P 		1077
avr 1410.4
(3) 		755.6
(-0.6) 		784.1
(-0.4) 		315.5
(-3) 		1095

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1382
1 2 3 4 5 6 range
A 918.9
(-2.7)
P 		1930.5
(3)
P 		1362.4
(-0.2)
P 		1324.7
(-0.4)
P 		1488.6
(0.5)
P 		856.8
(-3)
P 		1074
B 1541.4
(-1)
P 		1778.3
(0.3)
P 		1642.6
(-0.5)
P 		1215.4
(-2.9)
P 		2238.7
(3)
P 		1202.5
(-3)
P 		1036
C 1535.5
(0.9)
P 		1657.9
(1.7)
P 		1834.7
(3)
P 		994.9
(-3)
P 		1650.6
(1.7)
P 		1152.6
(-1.9)
P 		840
avr 1331.9
(-0.8) 		1788.9
(3) 		1613.2
(1.5) 		1178.3
(-2.1) 		1792.6
(3) 		1070.6
(-3) 		722
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180913_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5116
Sequence Type Exemplar sequence
Representative Public ID F32A5.5 NCBI
Target Description F32A5.5 /REP_DB=WormBase Gene ID /WP=CE01275 /TR=Q19949 /GB=AAC46664.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063109(11), NM_182133(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063109 NCBI Caenorhabditis elegans AQuaPorin or aquaglyceroporin related family member (aqp-1) (aqp-1) mRNA, complete cds. 11/11 None
NM_182133 NCBI Caenorhabditis elegans AQuaPorin or aquaglyceroporin related family member (aqp-1) (aqp-1) mRNA, complete cds. 11/11 None
SNAP00000015811 ENSEMBL cdna:SNAP chromosome:CEL140:II:7235859:7236962:-1 11/11 None
GENEFINDER00000015825 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7235859:7236962:-1 11/11 None
F32A5.5a ENSEMBL cdna:known chromosome:CEL140:II:7235766:7238280:-1 gene:F32A5.5 11/11 None
F32A5.5b.2 ENSEMBL cdna:known chromosome:CEL140:II:7235859:7236971:-1 gene:F32A5.5 11/11 None
F32A5.5b.1 ENSEMBL cdna:known chromosome:CEL140:II:7235859:7236968:-1 gene:F32A5.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7235854-7236958(-) 100.0 100.0
Public Domain and Genome References
Ensembl F32A5.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC46664 Aquaporin or aquaglyceroporin related protein 1, isoform a [Caenorhabditis elegans] ref|NP_495510.1| AQuaPorin or aquaglyceroporin related family member (aqp-1) [Caenorhabditis elegans] 1.0E-174
blast AAN65321 Aquaporin or aquaglyceroporin related protein 1, isoform b [Caenorhabditis elegans] ref|NP_871933.1| AQuaPorin or aquaglyceroporin related family member (aqp-1) [Caenorhabditis elegans] 1.0E-169
blast AAC46664 Aquaporin or aquaglyceroporin related protein 1, isoform a [Caenorhabditis elegans] ref|NP_495510.1| AQuaPorin or aquaglyceroporin related family member (aqp-1) [Caenorhabditis elegans] 1.0E-169
Protein Domains
Database ID Description E-Value
Pfam IPR000425 EMBL-EBI Major intrinsic protein 1.2E-31
Pfam IPR000425 EMBL-EBI Major intrinsic protein 1.2E-31
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495510.1 6 30-52,67-89,110-132,167-189,202-224,252-274
NP_871933.1 6 21-43,58-80,101-123,158-180,193-215,243-265
F32A5.5a 6 30-52,67-89,110-132,167-189,202-224,252-274
F32A5.5b.2 6 21-43,58-80,101-123,158-180,193-215,243-265
F32A5.5b.1 6 21-43,58-80,101-123,158-180,193-215,243-265
SNAP00000015811 6 21-43,58-80,101-123,158-180,193-215,243-265
GENEFINDER00000015825 6 21-43,58-80,101-123,158-180,193-215,243-265
Sequence
Target Sequence 
>C. ELEGANS:180913_AT
gtctcttggtcatctcaaacttgcttgggtgccactgtacgcaattgctcaaaccgctgg
agctttcgtggcttctcttggaatgtactcttattactatgaacaattcaatgcatttga
tggaggtaatcgcacaatcctcggagctactggaacagctggttgctttgccagttatcc
atctccaaatcttggagtatggggtccatacattgatcagtgcgtcggtaccggagttct
cgcatacttcctctgcgttgttattgatgagagaaatcaaattccaaaaatttggcatcc
aatgttcttcggattcttggtaatgatgatcggaactggatttggaatgaacattggata
tccaattaatccagcaagagatctcggaccacgactcttcagttattttatctatggtcc
aggtgttttccactctccataccctaactattggctagctccagccattgctccatttgt
tggtgctcttgtcggtggatggttctatcatttctccctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTCTTGGTCATCTCAAACTTGCT 78 471 288 Antisense
CTTGGGTGCCACTGTACGCAATTGC 549 199 311 Antisense
CTCAAACCGCTGGAGCTTTCGTGGC 695 225 335 Antisense
GTAATCGCACAATCCTCGGAGCTAC 17 463 413 Antisense
TGGTTGCTTTGCCAGTTATCCATCT 468 551 447 Antisense
GGGTCCATACATTGATCAGTGCGTC 487 497 489 Antisense
TCAGTGCGTCGGTACCGGAGTTCTC 427 621 504 Antisense
ATACTTCCTCTGCGTTGTTATTGAT 524 23 531 Antisense
GGACCACGACTCTTCAGTTATTTTA 522 511 673 Antisense
CACTCTCCATACCCTAACTATTGGC 98 199 718 Antisense
TGGATGGTTCTATCATTTCTCCCTT 661 549 783 Antisense
Sequence Source Affymetrix Proprietary Database