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[180903_at]

Description F40G12.8 /REP_DB=WormBase Gene ID /WP=CE10190 /TR=Q20244 /GB=CAB01181.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 72      4grp_total > 12      6grp_cgc > 75      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 11
(-2.9)
A 		31.2
(-1.3)
A 		10.1
(-3)
A 		83.1
(3)
P 		73
B 30
(-1.8)
A 		27.7
(-2.2)
A 		23.2
(-3)
A 		56.4
(3)
P 		33
C 57.4
(1.6)
A 		11.1
(-3)
A 		16.5
(-2.5)
A 		71.6
(3)
P 		61
avr 32.8
(-1.2) 		23.3
(-2.3) 		16.6
(-3) 		70.4
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 2.7
(-3)
A 		11.3
(-2.2)
A 		70
(3)
A 		28.6
(-0.7)
A 		24.4
(-1.1)
A 		27.5
(-0.8)
A 		67
B 30.5
(2.3)
A 		32.3
(3)
A 		17.3
(-3)
A 		18.5
(-2.5)
A 		26.1
(0.5)
A 		24.3
(-0.2)
A 		15
C 1
(-3)
A 		47.9
(2.2)
P 		29.4
(0.2)
A 		54.7
(3)
A 		48.8
(2.3)
A 		15.4
(-1.4)
A 		54
avr 11.4
(-3) 		30.5
(1.2) 		38.9
(3) 		33.9
(1.9) 		33.1
(1.7) 		22.4
(-0.6) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 4
(-2.1)
A 		6.8
(-0.4)
A 		2.6
(-3)
A 		12.2
(3)
P 		10
B 9.6
(0.8)
A 		6.8
(-1.7)
A 		5.4
(-3)
A 		12
(3)
P 		7
C 19
(3)
A 		3.2
(-3)
A 		5.8
(-2)
A 		12.1
(0.4)
P 		16
avr 10.9
(2) 		5.6
(-2.2) 		4.6
(-3) 		12.1
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 0.5
(-3)
A 		4
(-1.6)
A 		15.2
(3)
A 		7.3
(-0.2)
A 		7.4
(-0.2)
A 		6.7
(-0.5)
A 		15
B 10.6
(2.9)
A 		10.7
(3)
A 		4.2
(-3)
A 		4.9
(-2.4)
A 		10.6
(2.9)
A 		9.1
(1.5)
A 		7
C 0.4
(-3)
A 		14.6
(2.5)
P 		7.5
(-0.2)
A 		11
(1.1)
A 		15.8
(3)
A 		5.2
(-1.1)
A 		15
avr 3.8
(-3) 		9.8
(1.8) 		9
(1.2) 		7.7
(0.1) 		11.3
(3) 		7
(-0.4) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180903_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12956
Sequence Type Exemplar sequence
Representative Public ID F40G12.8 NCBI
Target Description F40G12.8 /REP_DB=WormBase Gene ID /WP=CE10190 /TR=Q20244 /GB=CAB01181.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074134(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074134 NCBI Caenorhabditis elegans Serpentine Receptor, class T family member (srt-33) (srt-33) mRNA, complete cds. 11/11 None
SNAP00000026733 ENSEMBL cdna:SNAP chromosome:CEL140:V:14280900:14282465:-1 11/11 None
F40G12.8 ENSEMBL cdna:known chromosome:CEL140:V:14280900:14282465:-1 gene:F40G12.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14281024-14284910(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srt-33
Ensembl F40G12.8
Entrez Gene 185568 Entrez gene
SwissProt Q20244 EMBL-EBI
WormBase CE37836 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB01181 Hypothetical protein F40G12.8 [Caenorhabditis elegans] ref|NP_506535.2| Serpentine Receptor, class T family member (srt-33) [Caenorhabditis elegans] 0.0
blast AAC78202 Serpentine receptor, class t protein 34 [Caenorhabditis elegans] ref|NP_503763.1| Serpentine Receptor, class T family member (srt-34) [Caenorhabditis elegans] 1.0E-162
blast CAI46604 Hypothetical protein F40G12.15 [Caenorhabditis elegans] ref|NP_001023908.1| F40G12.15 [Caenorhabditis elegans] 1.0E-150
Protein Domains
Database ID Description E-Value
Pfam IPR002651 EMBL-EBI Protein of unknown function DUF32 8.3E-116
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506535.2 7 40-62,75-97,112-134,155-177,203-225,246-265,275-297
F40G12.8 7 40-62,75-97,112-134,155-177,203-225,246-265,275-297
SNAP00000026733 7 40-62,75-97,112-134,155-177,203-225,246-265,275-297
Sequence
Target Sequence 
>C. ELEGANS:180903_AT
ttttggtattgttcatgtctcactacaattctgcttgttgctaataggtcattagacttg
acatttcctgattttgggcgtgtgatatttgatggtaaaaagacctacattctcttgatc
tttccaattgtctatggaatatggtttctatggtacaacgcaccaattgtcttcacttca
acgtttcattcttggttttttgatccaatgatttttgagggaagggcagtggaatatgat
aactttcctgttttcttcaacaatttattggtcgtggtgtctacttgctttttgtatgca
ttgttttgtactgttcttattgcaaaagggaaacatgcaaaagcaggatcaacatctaaa
acggtgagtcgacaaatagtgttccaatcgacactcatatgtgctgtaaacttccttaca
tctgcaatttatgtcatagagaattacatagcagttcctttttggattgtgataagcggt
caattttgttggcaactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTGGTATTGTTCATGTCTCACTA 332 679 1327 Antisense
GTCTCACTACAATTCTGCTTGTTGC 694 469 1343 Antisense
GACATTTCCTGATTTTGGGCGTGTG 148 371 1386 Antisense
CCTACATTCTCTTGATCTTTCCAAT 379 267 1430 Antisense
GGTACAACGCACCAATTGTCTTCAC 129 505 1478 Antisense
AATTGTCTTCACTTCAACGTTTCAT 497 179 1491 Antisense
TTATTGGTCGTGGTGTCTACTTGCT 172 679 1591 Antisense
GTGTCTACTTGCTTTTTGTATGCAT 402 487 1603 Antisense
AAACTTCCTTACATCTGCAATTTAT 559 127 1734 Antisense
ATTACATAGCAGTTCCTTTTTGGAT 326 13 1769 Antisense
AAGCGGTCAATTTTGTTGGCAACTT 607 147 1800 Antisense
Sequence Source Affymetrix Proprietary Database