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[180902_s_at]

Description F25B5.7 /REP_DB=WormBase Gene ID /WP=CE01924 /TR=Q09542 /GB=AAC46528.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 19      4grp_highest_8_8 > 05      4grp_high_8_8 > 29      6grp_total > 29      4grp_total > 33      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 349
1 2 3 4 range
A 510.8
(-3)
P 		849.8
(2.9)
P 		654.6
(-0.5)
P 		855.5
(3)
P 		345
B 656.7
(-3)
P 		655.3
(-3)
P 		728.4
(-0.9)
P 		859.8
(3)
P 		205
C 605.3
(-3)
P 		789.6
(3)
P 		715.7
(0.6)
P 		623.1
(-2.4)
P 		184
avr 590.9
(-3) 		764.9
(2.5) 		699.6
(0.5) 		779.5
(3) 		189

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 483
1 2 3 4 5 6 range
A 494.7
(-2.3)
P 		741.4
(3)
P 		464
(-3)
P 		625.1
(0.5)
P 		525.5
(-1.7)
P 		726.9
(2.7)
P 		277
B 497.4
(-0.1)
P 		388.9
(-3)
P 		430.3
(-1.9)
P 		389.2
(-3)
P 		617.2
(3)
P 		509.3
(0.2)
P 		228
C 871.6
(3)
P 		632.1
(-0.1)
P 		537.2
(-1.3)
P 		460.6
(-2.3)
M 		694.7
(0.7)
P 		407.5
(-3)
P 		464
avr 621.2
(3) 		587.5
(1.6) 		477.2
(-3) 		491.6
(-2.4) 		612.5
(2.6) 		547.9
(-0.1) 		144
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 144
1 2 3 4 range
A 186
(3)
P 		184.5
(2.9)
P 		171.1
(1.5)
P 		125.2
(-3)
P 		61
B 209.4
(3)
P 		161.8
(-3)
P 		168.8
(-2.1)
P 		183.2
(-0.3)
P 		48
C 200.4
(1)
P 		227
(2.1)
P 		249.5
(3)
P 		105.1
(-3)
P 		144
avr 198.6
(3) 		191.1
(2.3) 		196.5
(2.8) 		137.8
(-3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 232
1 2 3 4 5 6 range
A 91.2
(-3)
P 		263.7
(3)
P 		101
(-2.7)
P 		158.5
(-0.7)
P 		158.6
(-0.7)
P 		177
(-0)
P 		173
B 173.1
(-0.2)
P 		129.5
(-1.9)
P 		104.5
(-2.9)
P 		103.1
(-3)
P 		250.8
(3)
P 		189.9
(0.5)
P 		148
C 323.1
(3)
P 		192.9
(-0.4)
P 		136.7
(-1.9)
P 		92.6
(-3)
M 		224.8
(0.4)
P 		136.4
(-1.9)
P 		231
avr 195.8
(2) 		195.4
(2) 		114.1
(-3) 		118.1
(-2.8) 		211.4
(3) 		167.8
(0.3) 		97
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180902_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6843
Sequence Type Exemplar sequence
Representative Public ID F25B5.7 NCBI
Target Description F25B5.7 /REP_DB=WormBase Gene ID /WP=CE01924 /TR=Q09542 /GB=AAC46528.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065895(11), NM_065894(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065895 NCBI Caenorhabditis elegans F25B5.7c (F25B5.7) mRNA, complete cds. 11/11 None
NM_065894 NCBI Caenorhabditis elegans F25B5.7a (F25B5.7) mRNA, complete cds. 11/11 None
SNAP00000007786 ENSEMBL cdna:SNAP chromosome:CEL140:III:5953656:5958157:-1 11/11 None
GENEFINDER00000007796 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5953656:5958157:-1 11/11 None
F25B5.7c ENSEMBL cdna:known chromosome:CEL140:III:5953486:5958186:-1 gene:F25B5.7 11/11 None
F25B5.7a ENSEMBL cdna:known chromosome:CEL140:III:5953656:5958157:-1 gene:F25B5.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
F25B5.7b 2/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175844 4 2 NM_065894 180902_s_at (11)
NM_065895 180902_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5953654-5958156(-) 98.1 100.0
Public Domain and Genome References
Ensembl F25B5.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498295 F25B5.7a [Caenorhabditis elegans] gb|AAC46528.1| Hypothetical protein F25B5.7a [Caenorhabditis elegans] 1.0E-151
blast NP_871666 F25B5.7d [Caenorhabditis elegans] gb|AAN63408.1| Hypothetical protein F25B5.7d [Caenorhabditis elegans] 1.0E-151
blast CAE64347 Hypothetical protein CBG09034 [Caenorhabditis briggsae] 1.0E-134
Protein Domains
Database ID Description E-Value
Pfam IPR000504 EMBL-EBI RNA-binding region RNP-1 (RNA recognition motif) 1.4E-18
Pfam IPR000504 EMBL-EBI RNA-binding region RNP-1 (RNA recognition motif) 2.5E-9
Sequence
Target Sequence 
>C. ELEGANS:180902_S_AT
tccaacgccgccaacaagagctagaacgtcttgaagctgctcgccgcgaacgtatgcgtg
gtcttggtggaccaccaccaggaatgccaggaggtgtgccaccacaagctcagccattca
acccacacggaggatcaatgttcggcggacctggaggaccaggtggaccaccaccattcg
gtcaaccgttccaacaagcaccacaaggaatgttcaatgttcctacaggacctccaccac
tgatgggaggtggtggagatcctagaggaccaccaggtggaatgggaggaccaccaggac
acggaggaatgggaggtcccccaggacacggaggacaaggcggaccaccaggtcatggag
gaatgggaggaccaccaccaggacaaggagggccaggggtccgggagacctggaggtcct
ggaggaatgcttcaaaatctcacggacggagtaacatgatcgaaggagtccaacgattat
tgcagatcttcaaaagtgataatggaccaccaggaggaatgcccggccgtgattatccac
cacagcaaggaccaggaggcatgttcggatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCAACGCCGCCAACAAGAGCTAGA 387 587 1082 Antisense
GAACGTATGCGTGGTCTTGGTGGAC 121 349 1129 Antisense
CAGGAATGCCAGGAGGTGTGCCACC 205 209 1160 Antisense
CATTCAACCCACACGGAGGATCAAT 153 213 1196 Antisense
TCAATGTTCGGCGGACCTGGAGGAC 324 631 1216 Antisense
ATTCGGTCAACCGTTCCAACAAGCA 468 3 1257 Antisense
TTCAATGTTCCTACAGGACCTCCAC 426 685 1294 Antisense
AGATCCTAGAGGACCACCAGGTGGA 293 65 1338 Antisense
AGGACAAGGCGGACCACCAGGTCAT 225 55 1413 Antisense
GGAATGCTTCAAAATCTCACGGACG 91 533 1505 Antisense
AGGACCAGGAGGCATGTTCGGATCA 529 55 1629 Antisense
Sequence Source Affymetrix Proprietary Database