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[180893_s_at]

Description R12E2.1 /REP_DB=WormBase Gene ID /WP=CE18133 /TR=O61795 /GB=AAC17022.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 87      4grp_high_8_8 > 11      6grp_total > 87      4grp_total > 13      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 195
1 2 3 4 range
A 305.4
(1.2)
P 		252.8
(-0.4)
P 		167.7
(-3)
M 		362.8
(3)
P 		195
B 234.9
(-1.2)
P 		183.2
(-3)
P 		182.5
(-3)
P 		359.6
(3)
P 		177
C 255.8
(1.9)
P 		168.2
(-3)
A 		203.5
(-1)
P 		275.9
(3)
P 		108
avr 265.4
(0.3) 		201.4
(-2.3) 		184.6
(-3) 		332.8
(3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 154
1 2 3 4 5 6 range
A 137.6
(-2.7)
A 		146.3
(-2.3)
P 		130.4
(-3)
P 		186.5
(-0.5)
P 		175.9
(-1)
P 		264.9
(3)
P 		135
B 177.2
(-0.8)
P 		186.6
(-0.2)
P 		142.8
(-3)
P 		213.9
(1.6)
P 		235.3
(3)
P 		216.6
(1.8)
P 		93
C 169.1
(-1.8)
P 		193.3
(-0.8)
P 		188.1
(-1)
P 		139.5
(-3)
A 		188.2
(-1)
P 		284.8
(3)
P 		145
avr 161.3
(-2.6) 		175.4
(-1.7) 		153.8
(-3) 		180
(-1.5) 		199.8
(-0.3) 		255.4
(3) 		102
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 111.2
(3)
P 		54.9
(-2)
P 		43.8
(-3)
M 		53.1
(-2.2)
P 		67
B 74.9
(2.7)
P 		45.3
(-2.5)
P 		42.3
(-3)
P 		76.6
(3)
P 		34
C 84.7
(3)
P 		48.4
(-2.7)
A 		70.9
(0.8)
P 		46.5
(-3)
P 		38
avr 90.3
(3) 		49.5
(-3) 		52.3
(-2.6) 		58.7
(-1.6) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 25.4
(-3)
A 		52
(1.1)
P 		28.4
(-2.5)
P 		47.3
(0.4)
P 		53.1
(1.3)
P 		64.5
(3)
P 		39
B 61.6
(-0.3)
P 		62.1
(-0.3)
P 		34.7
(-3)
P 		56.7
(-0.8)
P 		95.6
(3)
P 		80.8
(1.5)
P 		61
C 62.7
(0.1)
P 		59
(-0.2)
P 		47.9
(-1.2)
P 		28.1
(-3)
A 		60.9
(-0.1)
P 		95.3
(3)
P 		67
avr 49.9
(-1.2) 		57.7
(-0.1) 		37
(-3) 		44
(-2) 		69.9
(1.6) 		80.2
(3) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180893_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.418
Sequence Type Exemplar sequence
Representative Public ID R12E2.1 NCBI
Target Description R12E2.1 /REP_DB=WormBase Gene ID /WP=CE18133 /TR=O61795 /GB=AAC17022.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058921(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058921 NCBI Caenorhabditis elegans R12E2.1 (R12E2.1) mRNA, complete cds. 11/11 None
SNAP00000012687 ENSEMBL cdna:SNAP chromosome:CEL140:I:4182222:4184917:1 10/11 None
R12E2.1.2 ENSEMBL cdna:known chromosome:CEL140:I:4182220:4184990:1 gene:R12E2.1 11/11 None
R12E2.1.1 ENSEMBL cdna:known chromosome:CEL140:I:4182222:4184990:1 gene:R12E2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4182221-4184688(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R12E2.1
Entrez Gene 172012 Entrez gene
SwissProt O61795 EMBL-EBI
WormBase CE18133 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1598492_AT similar to kelch-like 20 cfa
CANINE:1597100_AT similar to kelch-like 20 cfa
CANINE_2:CFA.13460.1.A1_AT similar to kelch-like 20 cfa
CANINE_2:CFA.6026.1.A1_S_AT similar to kelch-like 20 cfa
CANINE_2:CFAAFFX.22432.1.S1_S_AT similar to kelch-like 20 cfa
DROSOPHILA_2:1625931_AT diablo dm
DROSGENOME1:148826_AT diablo dm
CHICKEN:GGAAFFX.8370.1.S1_S_AT similar to Kelch-like protein X gga
HG-U133_PLUS_2:210634_AT kelch-like 20 (Drosophila) hs
HG-U133A:210634_AT kelch-like 20 (Drosophila) hs
HG-U133A_2:210634_AT kelch-like 20 (Drosophila) hs
HG-U133A:204177_S_AT kelch-like 20 (Drosophila) hs
HG-U133_PLUS_2:204177_S_AT kelch-like 20 (Drosophila) hs
HG-U133A_2:204177_S_AT kelch-like 20 (Drosophila) hs
HG-U133_PLUS_2:210635_S_AT kelch-like 20 (Drosophila) hs
HG-U133A:210635_S_AT kelch-like 20 (Drosophila) hs
HG-U133A_2:210635_S_AT kelch-like 20 (Drosophila) hs
HU35KSUBC:RC_AA486552_AT kelch-like 20 (Drosophila) hs
HU35KSUBD:RC_AA479295_AT kelch-like 20 (Drosophila) hs
U133_X3P:G13677223_3P_A_AT kelch-like 20 (Drosophila) hs
U133_X3P:G13528917_3P_AT kelch-like 20 (Drosophila) hs
U133_X3P:G13528917_3P_A_AT kelch-like 20 (Drosophila) hs
HG-U133A_2:204176_AT kelch-like 20 (Drosophila) hs
HG-FOCUS:204176_AT kelch-like 20 (Drosophila) hs
HG-U133_PLUS_2:204176_AT kelch-like 20 (Drosophila) hs
HG-U133A:204176_AT kelch-like 20 (Drosophila) hs
HG-U95AV2:37150_AT kelch-like 20 (Drosophila) hs
HG-U95B:43075_R_AT kelch-like 20 (Drosophila) hs
HG-U133_PLUS_2:232906_AT Kelch-like 20 (Drosophila) hs
HG-U133B:232906_AT Kelch-like 20 (Drosophila) hs
U133_X3P:HS.287429.0.S1_3P_AT Kelch-like 20 (Drosophila) hs
U133_X3P:HS.106290.0.S2_3P_AT Kelch-like 20 (Drosophila) hs
MOUSE430_2:1425664_AT kelch-like 20 (Drosophila) mm
MOUSE430A_2:1425664_AT kelch-like 20 (Drosophila) mm
MOE430A:1425664_AT kelch-like 20 (Drosophila) mm
MG-U74BV2:113686_AT kelch-like 20 (Drosophila) mm
MG-U74CV2:134805_AT kelch-like 20 (Drosophila) mm
MOE430B:1454994_AT kelch-like 20 (Drosophila) mm
MOUSE430_2:1454994_AT kelch-like 20 (Drosophila) mm
MU19KSUBC:TC36560_AT Kelch-like 20 (Drosophila) (Klhl20), mRNA mm
RAE230B:1395182_AT similar to Kelch-like protein X (predicted) rn
RAT230_2:1395182_AT similar to Kelch-like protein X (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5515 protein binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_491322 R12E2.1 [Caenorhabditis elegans] gb|AAC17022.1| Hypothetical protein R12E2.1 [Caenorhabditis elegans] 0.0
blast CAE66743 Hypothetical protein CBG12093 [Caenorhabditis briggsae] 0.0
blast XP_789626 PREDICTED: similar to kelch-like 20 [Strongylocentrotus purpuratus] 0.0
blast XP_789626 PREDICTED: similar to kelch-like 20 [Strongylocentrotus purpuratus] 1.0E-180
Protein Domains
Database ID Description E-Value
Pfam IPR006652 EMBL-EBI Kelch repeat 1.7E-5
Pfam IPR006652 EMBL-EBI Kelch repeat 2.2E-16
Pfam IPR006652 EMBL-EBI Kelch repeat 9.9E-17
Pfam IPR006652 EMBL-EBI Kelch repeat 1.4E-17
Pfam IPR006652 EMBL-EBI Kelch repeat 0.24
Pfam IPR006652 EMBL-EBI Kelch repeat 1.6E-15
Pfam IPR000210 EMBL-EBI BTB/POZ domain 3.6E-44
Pfam IPR006652 EMBL-EBI Kelch repeat 3.8E-7
Sequence
Target Sequence 
>C. ELEGANS:180893_S_AT
atcgagcgcctggatccaatgaaaggaggaaccacgtggaagtgtgtggctccgatggga
aaacgaagatgtggagttggagttgctgttctggagaatcttttgtacgctgtgggtggt
cacgatggacaatcatacttgaatagtatagaaagatacgatccaatgaccaatcaatgg
agtagtgatgtggcaccaactgccacatgtcgaacgagtgttggagtagctgctttcaac
ggatctctgtatgctgtaggaggtcaagatggagaaagttgtcttgatgttgtcgaaaag
tatgatccacggaaaaacgagtgggcgaaagtggccagtatgggaactagaaggctagga
gtttcggtttcagttgttaatggttgcatttacgcagttggtggatcaaatggacccgca
cctctgaatactgtagaaaggtatgatcctcgagttggaaagtgggaagaagttcgaccg
atgctaaccaaacgaaagcatcttggaacagcggtttatgatggatacatttatgcggtt
ggtggaagagataccactacagaactcaacacggcgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGAGCGCCTGGATCCAATGAAAG 68 37 979 Antisense
GTGGAAGTGTGTGGCTCCGATGGGA 238 491 1014 Antisense
GTGGAGTTGGAGTTGCTGTTCTGGA 274 491 1049 Antisense
GAATCTTTTGTACGCTGTGGGTGGT 643 327 1074 Antisense
CCAACTGCCACATGTCGAACGAGTG 402 275 1174 Antisense
ACGAGTGTTGGAGTAGCTGCTTTCA 444 93 1192 Antisense
GCTTTCAACGGATCTCTGTATGCTG 61 305 1210 Antisense
GGCTAGGAGTTTCGGTTTCAGTTGT 548 537 1331 Antisense
ACCCGCACCTCTGAATACTGTAGAA 467 89 1392 Antisense
GAAGTTCGACCGATGCTAACCAAAC 330 335 1447 Antisense
ACCACTACAGAACTCAACACGGCGG 654 83 1531 Antisense
Sequence Source Affymetrix Proprietary Database