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[180866_at]

Description T05H4.2 /REP_DB=WormBase Gene ID /WP=CE13271 /TR=O16525 /GB=AAB66009.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 86      4grp_total > 24      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 18.2
(-3)
A 		25.3
(-0.6)
A 		27.4
(0.1)
P 		35.9
(3)
A 		18
B 27
(-1.3)
A 		36.5
(2.6)
A 		37.6
(3)
A 		22.7
(-3)
M 		15
C 19.4
(-2.8)
A 		18.7
(-3)
A 		24.6
(-1.7)
A 		45.8
(3)
A 		27
avr 21.5
(-3) 		26.8
(-0.6) 		29.9
(0.8) 		34.8
(3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 47.3
(-0.3)
P 		25.9
(-3)
P 		30.4
(-2.4)
A 		29.1
(-2.6)
A 		29.1
(-2.6)
P 		73.2
(3)
P 		47
B 2.1
(-3)
A 		19.9
(-1)
A 		20.6
(-0.9)
A 		39.1
(1.2)
A 		44.3
(1.8)
P 		55.4
(3)
P 		53
C 14
(-1.7)
A 		27.1
(-0.4)
A 		2
(-3)
A 		46.9
(1.7)
A 		24.6
(-0.6)
A 		59
(3)
P 		57
avr 21.1
(-2.5) 		24.3
(-2.1) 		17.7
(-3) 		38.4
(-0.2) 		32.7
(-1) 		62.5
(3) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 6.6
(1.1)
A 		5.5
(-2.4)
A 		7.2
(3)
P 		5.3
(-3)
A 		2
B 8.6
(2.4)
A 		9
(3)
A 		8.7
(2.6)
A 		4.8
(-3)
M 		4
C 6.4
(-1.1)
A 		5.4
(-3)
A 		8.6
(3)
A 		7.7
(1.3)
A 		3
avr 7.2
(0.4) 		6.6
(-1.2) 		8.2
(3) 		5.9
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 8.7
(-1.9)
P 		9.2
(-1.6)
P 		6.6
(-3)
A 		7.4
(-2.6)
A 		8.8
(-1.8)
P 		17.8
(3)
P 		11
B 0.7
(-3)
A 		6.6
(-1.2)
A 		5
(-1.7)
A 		10.4
(-0.1)
A 		18
(2.2)
P 		20.7
(3)
P 		20
C 5.2
(-1.5)
A 		8.3
(-0.6)
A 		0.5
(-3)
A 		9.4
(-0.2)
A 		8
(-0.7)
A 		19.8
(3)
P 		19
avr 4.9
(-2.6) 		8
(-1.4) 		4
(-3) 		9.1
(-1) 		11.6
(-0) 		19.4
(3) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180866_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12049
Sequence Type Exemplar sequence
Representative Public ID T05H4.2 NCBI
Target Description T05H4.2 /REP_DB=WormBase Gene ID /WP=CE13271 /TR=O16525 /GB=AAB66009.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072241(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072241 NCBI Caenorhabditis elegans T05H4.2 (T05H4.2) mRNA, complete cds. 11/11 None
SNAP00000040312 ENSEMBL cdna:SNAP chromosome:CEL140:V:6445159:6452774:1 11/11 None
T05H4.2 ENSEMBL cdna:known chromosome:CEL140:V:6451255:6452774:1 gene:T05H4.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000040315 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6451301-6452774(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T05H4.2
Entrez Gene 188148 Entrez gene
SwissProt O16525 EMBL-EBI
WormBase CE13271 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_504642 T05H4.2 [Caenorhabditis elegans] gb|AAB66009.1| Hypothetical protein T05H4.2 [Caenorhabditis elegans] 0.0
blast NP_504641 T05H4.3 [Caenorhabditis elegans] gb|AAB66021.1| Hypothetical protein T05H4.3 [Caenorhabditis elegans] 0.0
blast CAE71884 Hypothetical protein CBG18939 [Caenorhabditis briggsae] 0.0
blast CAB07161 Hypothetical protein C06H5.1 [Caenorhabditis elegans] ref|NP_741646.1| C06H5.1 [Caenorhabditis elegans] 1.0E-125
blast CAB07162 Hypothetical protein C06H5.2 [Caenorhabditis elegans] ref|NP_507510.1| C06H5.2 [Caenorhabditis elegans] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.7E-36
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 7.6E-6
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.022
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.46
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.9
Pfam IPR002900 EMBL-EBI Protein of unknown function DUF38 2.7E-36
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 7.6E-6
Sequence
Target Sequence 
>C. ELEGANS:180866_AT
gaagagttcgtttcatttgatgctattaacgacgttcttaaatcaattggaatcctgtcg
gcaaaatcagtattgctcgattggtttcacacttttgaaattactgtacttctgaaatac
tttaagcctggaatcctggaggacttaacaattaaatgtcttgaatccgatgaagagttc
acaggaaatctggtgtacctggaacagtggaaaaaggcaaagaatctgaaattgtctaaa
gtcccaaattttccaattatcgtcgaacatctcgataacttcaaatctttgaacttcgac
ttccatggggaacctttctcaagagatgatatcatgaagcttcgagatgaggttcttcta
aaatcaaccaagtttgaatacttttcttgtaaaaatgtgaaattcaacacaaaaatgttc
gtgaaagtttttgatgctcgctcaactctattttgttcgtctggctcaattgtttatgaa
tcgtcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGAGTTCGTTTCATTTGATGCTA 268 339 673 Antisense
GTATTGCTCGATTGGTTTCACACTT 338 453 742 Antisense
TTAAGCCTGGAATCCTGGAGGACTT 149 685 794 Antisense
GGAAATCTGGTGTACCTGGAACAGT 128 527 856 Antisense
TGTCTAAAGTCCCAAATTTTCCAAT 77 559 905 Antisense
TTCCAATTATCGTCGAACATCTCGA 121 701 923 Antisense
AAATCTTTGAACTTCGACTTCCATG 441 115 955 Antisense
CTTCCATGGGGAACCTTTCTCAAGA 343 219 972 Antisense
AGTTTTTGATGCTCGCTCAACTCTA 550 63 1098 Antisense
CAACTCTATTTTGTTCGTCTGGCTC 113 189 1115 Antisense
GGCTCAATTGTTTATGAATCGTCCA 265 539 1135 Antisense
Sequence Source Affymetrix Proprietary Database