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[180858_at]

Description T09B4.5 /REP_DB=WormBase Gene ID /WP=CE13465 /TR=O02155 /GB=AAB53007.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 58      4grp_highest_8_8 > 0      4grp_high_8_8 > 44      6grp_highest_nonorm > 84      6grp_total > 47      4grp_total > 51      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1118
1 2 3 4 range
A 810.4
(0.5)
P 		1281.6
(3)
P 		685.3
(-0.2)
P 		167
(-3)
P 		1115
B 854.2
(0.8)
P 		1248.5
(3)
P 		678.1
(-0.2)
P 		173.2
(-3)
P 		1075
C 745.1
(-0.1)
P 		1285.2
(3)
P 		711.2
(-0.3)
P 		241.5
(-3)
P 		1044
avr 803.2
(0.4) 		1271.8
(3) 		691.5
(-0.2) 		193.9
(-3) 		1078

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 818
1 2 3 4 5 6 range
A 833
(0.8)
P 		688.7
(-0.3)
P 		334.2
(-3)
P 		400.2
(-2.5)
P 		389.6
(-2.6)
P 		1128.9
(3)
P 		795
B 694.1
(1.5)
P 		560.8
(-0.1)
P 		310.8
(-3)
P 		426.9
(-1.6)
P 		374.4
(-2.3)
P 		822.7
(3)
P 		512
C 801.6
(1.3)
P 		563.4
(-1)
P 		383.9
(-2.7)
P 		355.8
(-3)
P 		394.6
(-2.6)
P 		976.7
(3)
P 		621
avr 776.2
(1.1) 		604.3
(-0.5) 		343
(-3) 		394.3
(-2.5) 		386.2
(-2.6) 		976.1
(3) 		633
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 345
1 2 3 4 range
A 295
(3)
P 		278.2
(2.6)
P 		179.2
(0.4)
P 		24.4
(-3)
P 		271
B 272.4
(2.2)
P 		308.3
(3)
P 		157.1
(-0.3)
P 		36.9
(-3)
P 		271
C 246.6
(0.8)
P 		369.5
(3)
P 		247.9
(0.8)
P 		40.7
(-3)
P 		329
avr 271.3
(2) 		318.7
(3) 		194.7
(0.4) 		34
(-3) 		285

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 255
1 2 3 4 5 6 range
A 153.6
(-0.6)
P 		244.9
(2.1)
P 		72.8
(-3)
P 		101.5
(-2.1)
P 		117.6
(-1.7)
P 		274.9
(3)
P 		202
B 241.5
(1.3)
P 		186.7
(-0.1)
P 		75.5
(-3)
P 		113.1
(-2)
P 		152.1
(-1)
P 		306.8
(3)
P 		231
C 297.1
(2.3)
P 		171.9
(-0.6)
P 		97.7
(-2.4)
P 		71.5
(-3)
P 		127.7
(-1.7)
P 		326.9
(3)
P 		255
avr 230.7
(1) 		201.2
(0.2) 		82
(-3) 		95.4
(-2.6) 		132.5
(-1.6) 		302.9
(3) 		221
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180858_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1995
Sequence Type Exemplar sequence
Representative Public ID T09B4.5 NCBI
Target Description T09B4.5 /REP_DB=WormBase Gene ID /WP=CE13465 /TR=O02155 /GB=AAB53007.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059373(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059373 NCBI Caenorhabditis elegans T09B4.5a (T09B4.5) mRNA, complete cds. 11/11 None
GENEFINDER00000036044 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6157801:6165155:1 11/11 None
SNAP00000036034 ENSEMBL cdna:SNAP chromosome:CEL140:I:6163224:6164929:1 11/11 None
T09B4.5a.1 ENSEMBL cdna:known chromosome:CEL140:I:6162206:6165556:1 gene:T09B4.5 11/11 None
T09B4.5a.2 ENSEMBL cdna:known chromosome:CEL140:I:6162210:6164929:1 gene:T09B4.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182036 4/11 Cross Hyb Matching Probes None
T09B4.5b 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172300 2 1 NM_059373 174789_at (11), 180858_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6163482-6164858(+) 99.8 99.8
Public Domain and Genome References
Ensembl T09B4.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB53007 Hypothetical protein T09B4.5a [Caenorhabditis elegans] ref|NP_491774.1| T09B4.5a [Caenorhabditis elegans] 1.0E-135
blast AAB53007 Hypothetical protein T09B4.5a [Caenorhabditis elegans] ref|NP_491774.1| T09B4.5a [Caenorhabditis elegans] 1.0E-134
blast AAS80340 Hypothetical protein F56F4.1 [Caenorhabditis elegans] 1.0E-132
blast NP_001021506 F56F4.1 [Caenorhabditis elegans] 1.0E-127
blast AAO21395 Hypothetical protein T09B4.5b [Caenorhabditis elegans] ref|NP_871836.1| T09B4.5b [Caenorhabditis elegans] 9.0E-41
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491774.1 2 209-231,246-268
T09B4.5a.1 2 209-231,246-268
T09B4.5a.2 2 209-231,246-268
GENEFINDER00000036044 2 485-507,520-542
SNAP00000036034 2 218-240,253-275
Sequence
Target Sequence 
>C. ELEGANS:180858_AT
taccacgtgcagttcatcacacatttgaaggtcttccagtacgcaccacccgttcagctt
caaaagcccagaaatctcaatgctgtgcttttctgacaaatgccaaagctcacgctatct
ccgcatacaactggatcgctgctcttctctccacaatcttcgcctatatccactacatca
tcacagcgccattcgtgttccttcgcaatcaatggcgtctgaacaggcctgagaataact
ggctcgctgctgcgatgatcattgtcttgtgcacatcgttctgtgttatcttcttccttg
cattcccaaagtttgtagacagatgctacgtgtatggtcagaagcacatcgccgtgccag
ctcaacgcttccagcgtcagacctactcgaaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACCACGTGCAGTTCATCACACATT 86 651 509 Antisense
ATCTCAATGCTGTGCTTTTCTGACA 549 31 582 Antisense
AAATGCCAAAGCTCACGCTATCTCC 500 119 606 Antisense
TCCGCATACAACTGGATCGCTGCTC 621 595 628 Antisense
TATATCCACTACATCATCACAGCGC 239 661 673 Antisense
TTCGTGTTCCTTCGCAATCAATGGC 329 695 700 Antisense
ATAACTGGCTCGCTGCTGCGATGAT 309 25 743 Antisense
GATCATTGTCTTGTGCACATCGTTC 244 421 765 Antisense
TCTTCCTTGCATTCCCAAAGTTTGT 79 617 800 Antisense
TGGTCAGAAGCACATCGCCGTGCCA 419 551 843 Antisense
TTCCAGCGTCAGACCTACTCGAAGA 339 701 877 Antisense
Sequence Source Affymetrix Proprietary Database