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[180842_s_at]

Description C33A12.7 /REP_DB=WormBase Gene ID /WP=CE15664 /TR=O17636 /GB=CAA92800.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 73      4grp_highest_8_8 > 61      4grp_high_8_8 > 18      6grp_highest_nonorm > 63      6grp_total > 73      4grp_total > 30      6grp_highest_sub > 04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 269
1 2 3 4 range
A 513.9
(3)
P 		378.7
(-1)
P 		313.4
(-3)
P 		423.5
(0.3)
P 		201
B 408.1
(3)
P 		317.2
(-0.3)
P 		244.7
(-3)
P 		394.5
(2.5)
P 		163
C 479.9
(3)
P 		321.6
(-3)
P 		324.6
(-2.9)
P 		422.8
(0.8)
P 		158
avr 467.3
(3) 		339.2
(-1.4) 		294.2
(-3) 		413.6
(1.1) 		173

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 565
1 2 3 4 5 6 range
A 355.3
(-3)
P 		507.9
(-1.4)
P 		877
(2.5)
P 		919.8
(3)
P 		622.3
(-0.2)
P 		757.2
(1.3)
P 		565
B 356
(-3)
P 		528.9
(-0.3)
P 		733.9
(3)
P 		710.3
(2.6)
P 		722.9
(2.8)
P 		676.4
(2.1)
P 		378
C 410.6
(-3)
P 		517.8
(-1.7)
P 		753.8
(1.2)
P 		905.2
(3)
P 		576
(-1)
P 		636.4
(-0.3)
P 		495
avr 374
(-3) 		518.2
(-1.2) 		788.2
(2.3) 		845.1
(3) 		640.4
(0.4) 		690
(1) 		471
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 187.1
(3)
P 		82.2
(-2)
P 		81.9
(-2)
P 		62
(-3)
P 		125
B 130.1
(3)
P 		78.3
(-1.2)
P 		56.7
(-3)
P 		84.1
(-0.8)
P 		73
C 158.9
(3)
P 		92.5
(-1.5)
P 		113.1
(-0.1)
P 		71.3
(-3)
P 		88
avr 158.7
(3) 		84.3
(-2.2) 		83.9
(-2.2) 		72.5
(-3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 228
1 2 3 4 5 6 range
A 65.5
(-3)
P 		180.6
(1.1)
P 		191
(1.5)
P 		233.3
(3)
P 		187.8
(1.4)
P 		184.3
(1.2)
P 		168
B 123.9
(-3)
P 		176.1
(-1.2)
P 		178.2
(-1.1)
P 		188.1
(-0.7)
P 		293.7
(3)
P 		252.2
(1.5)
P 		170
C 152.2
(-3)
P 		158
(-2.4)
P 		191.9
(0.9)
P 		182
(-0.1)
P 		186.4
(0.4)
P 		213
(3)
P 		61
avr 113.9
(-3) 		171.6
(0.2) 		187
(1) 		201.1
(1.8) 		222.6
(3) 		216.5
(2.7) 		109
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180842_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8433
Sequence Type Exemplar sequence
Representative Public ID C33A12.7 NCBI
Target Description C33A12.7 /REP_DB=WormBase Gene ID /WP=CE15664 /TR=O17636 /GB=CAA92800.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069283(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069283 NCBI Caenorhabditis elegans C33A12.7 (C33A12.7) mRNA, complete cds. 11/11 None
SNAP00000009026 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9506970:9507527:-1 9/11 None
GENEFINDER00000009042 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9504085:9508675:-1 9/11 None
C33A12.7 ENSEMBL cdna:known chromosome:CEL140:IV:9506970:9508729:-1 gene:C33A12.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000009027 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9506975-9508681(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C33A12.7
Entrez Gene 177783 Entrez gene
SwissProt O17636 EMBL-EBI
WormBase CE15664 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260986_AT hydroxyacylglutathione hydrolase, putative / glyoxalase II, putative at
ATGENOME1:15625_AT hydroxyacylglutathione hydrolase, putative / glyoxalase II, putative at
CANINE_2:CFAAFFX.8070.1.S1_AT similar to ETHE1 protein cfa
DROSOPHILA_2:1632652_S_AT dm
DROSOPHILA_2:1628558_AT dm
HG-U133_PLUS_2:204034_AT ethylmalonic encephalopathy 1 hs
HG-U133A:204034_AT ethylmalonic encephalopathy 1 hs
HG-FOCUS:204034_AT ethylmalonic encephalopathy 1 hs
HG-U133A_2:204034_AT ethylmalonic encephalopathy 1 hs
HU35KSUBA:RC_AA256365_S_AT ethylmalonic encephalopathy 1 hs
HU35KSUBC:RC_AA155749_AT ethylmalonic encephalopathy 1 hs
U133_X3P:G7657686_3P_AT ethylmalonic encephalopathy 1 hs
HG-U95AV2:36170_AT ethylmalonic encephalopathy 1 hs
MG-U74AV2:95660_AT ethylmalonic encephalopathy 1 mm
MOE430A:1417203_AT ethylmalonic encephalopathy 1 mm
MOUSE430A_2:1417203_AT ethylmalonic encephalopathy 1 mm
MOUSE430_2:1417203_AT ethylmalonic encephalopathy 1 mm
MG-U74CV2:133287_AT Ethylmalonic encephalopathy 1, mRNA (cDNA clone MGC:7046 IMAGE:3156338) mm
MU19KSUBB:TC31336_AT Ethylmalonic encephalopathy 1, mRNA (cDNA clone MGC:7046 IMAGE:3156338) mm
RG-U34C:RC_AI100882_AT ethylmalonic encephalopathy 1 (predicted) rn
RAE230A:1372306_AT ethylmalonic encephalopathy 1 (predicted) rn
RAT230_2:1372306_AT ethylmalonic encephalopathy 1 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAA92800 Hypothetical protein C33A12.7 [Caenorhabditis elegans] ref|NP_501684.1| C33A12.7 [Caenorhabditis elegans] 1.0E-126
blast CAE72759 Hypothetical protein CBG20009 [Caenorhabditis briggsae] 1.0E-108
blast CAA92800 Hypothetical protein C33A12.7 [Caenorhabditis elegans] ref|NP_501684.1| C33A12.7 [Caenorhabditis elegans] 1.0E-95
blast CAA92799 Hypothetical protein C33A12.16 [Caenorhabditis elegans] ref|NP_501682.2| C33A12.16 [Caenorhabditis elegans] 3.0E-90
blast CAE72759 Hypothetical protein CBG20009 [Caenorhabditis briggsae] 1.0E-82
blast CAA92800 Hypothetical protein C33A12.7 [Caenorhabditis elegans] ref|NP_501684.1| C33A12.7 [Caenorhabditis elegans] 2.0E-76
blast CAE72759 Hypothetical protein CBG20009 [Caenorhabditis briggsae] 9.0E-65
blast AAH41511 Ethe1-prov protein [Xenopus laevis] 1.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR001279 EMBL-EBI Beta-lactamase-like 1.5E-25
Pfam IPR001279 EMBL-EBI Beta-lactamase-like 1.1E-12
Pfam IPR001534 EMBL-EBI Transthyretin-like 1.6E-41
Sequence
Target Sequence 
>C. ELEGANS:180842_S_AT
catgctgatcatattactggaacaaatagcttgaaaacggtcttcccaacaatgaagtca
gtgctcagttcaaaatcgggtggcgaggctgataaatatgtttcggacggagaaatcatt
gaaattggtggactgaaattagaagtgagagagactccaggacatacaaatggatgtttg
acatatgtggaacattcattgagatctgcattcactggagatgcactgctcattcgagca
tgtggaagaactgattttcagcaaggaaacccggcatctctctttgactcggttcatgac
aaaatcttcacacttccagaagattatgttgtttatgttggacataactacaatggtgtt
ttgcaaactacggtctgggaagagaaaaatctgaatccacgtcttacgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATGCTGATCATATTACTGGAACAA 176 209 211 Antisense
GCTTGAAAACGGTCTTCCCAACAAT 698 299 239 Antisense
GTGCTCAGTTCAAAATCGGGTGGCG 571 477 271 Antisense
GTGAGAGAGACTCCAGGACATACAA 187 483 355 Antisense
TCATTGAGATCTGCATTCACTGGAG 122 619 406 Antisense
CTGGAGATGCACTGCTCATTCGAGC 41 241 425 Antisense
TTCAGCAAGGAAACCCGGCATCTCT 213 685 467 Antisense
TCTCTCTTTGACTCGGTTCATGACA 142 613 487 Antisense
ATGACAAAATCTTCACACTTCCAGA 324 45 506 Antisense
TGTTTTGCAAACTACGGTCTGGGAA 222 559 567 Antisense
AAAATCTGAATCCACGTCTTACGAA 503 133 596 Antisense
Sequence Source Affymetrix Proprietary Database