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[180833_s_at]

Description D1009.4 /REP_DB=WormBase Gene ID /WP=CE04288 /TR=Q18915 /GB=AAA81697.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 76      4grp_highest_8_8 > 39      4grp_high_8_8 > 04      6grp_highest_nonorm > 93      6grp_total > 67      4grp_total > 05      6grp_highest_sub > Da      6grp_cgc > 48      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1667
1 2 3 4 range
A 783.3
(-0.8)
P 		354.9
(-2.5)
P 		212.1
(-3)
P 		1771.2
(3)
P 		1559
B 934.3
(-0.6)
P 		319.6
(-2.9)
P 		287
(-3)
P 		1879.4
(3)
P 		1592
C 868.9
(-0.5)
P 		382.8
(-2.6)
P 		282.8
(-3)
P 		1668.3
(3)
P 		1386
avr 862.2
(-0.6) 		352.4
(-2.6) 		260.6
(-3) 		1773
(3) 		1512

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 425
1 2 3 4 5 6 range
A 241.7
(-3)
P 		318.3
(-1.6)
P 		326.9
(-1.5)
P 		253.8
(-2.8)
P 		307.2
(-1.8)
P 		572.9
(3)
P 		331
B 339.1
(-2.5)
P 		309.3
(-3)
P 		353.4
(-2.3)
P 		330.8
(-2.6)
P 		361.5
(-2.1)
P 		666.3
(3)
P 		357
C 356
(-1.1)
P 		343.4
(-1.4)
P 		325.5
(-1.7)
P 		262.1
(-3)
P 		312.5
(-2)
P 		566.2
(3)
P 		304
avr 312.3
(-2.4) 		323.7
(-2.2) 		335.3
(-2) 		282.2
(-3) 		327.1
(-2.2) 		601.8
(3) 		320
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 345
1 2 3 4 range
A 285.2
(3)
P 		77
(-2.4)
P 		55.5
(-3)
P 		259.3
(2.3)
P 		230
B 298
(1.2)
P 		78.9
(-2.8)
P 		66.5
(-3)
P 		400.5
(3)
P 		334
C 287.6
(3)
P 		110.1
(-2.6)
P 		98.5
(-3)
P 		281.3
(2.8)
P 		189
avr 290.3
(2.4) 		88.7
(-2.6) 		73.5
(-3) 		313.7
(3) 		240

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 204
1 2 3 4 5 6 range
A 44.6
(-3)
P 		113.2
(1.3)
P 		71.2
(-1.3)
P 		64.4
(-1.7)
P 		92.7
(0)
P 		139.5
(3)
P 		95
B 118
(-1.8)
P 		103
(-2.4)
P 		85.8
(-3)
P 		87.6
(-2.9)
P 		146.9
(-0.7)
P 		248.5
(3)
P 		163
C 132
(0.5)
P 		104.8
(-0.7)
P 		82.8
(-1.7)
P 		52.7
(-3)
P 		101.1
(-0.9)
P 		189.5
(3)
P 		137
avr 98.2
(-1.6) 		107
(-1.1) 		79.9
(-2.4) 		68.2
(-3) 		113.6
(-0.8) 		192.5
(3) 		124
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180833_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17124
Sequence Type Exemplar sequence
Representative Public ID D1009.4 NCBI
Target Description D1009.4 /REP_DB=WormBase Gene ID /WP=CE04288 /TR=Q18915 /GB=AAA81697.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077107(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077107 NCBI Caenorhabditis elegans Neuropeptide-Like Protein family member (nlp-14) (nlp-14) mRNA, complete cds. 11/11 None
SNAP00000025061 ENSEMBL cdna:SNAP chromosome:CEL140:X:8917678:8919329:-1 11/11 None
GENEFINDER00000025069 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8917678:8919329:-1 11/11 None
D1009.4 ENSEMBL cdna:known chromosome:CEL140:X:8917373:8919367:-1 gene:D1009.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8917676-8919328(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nlp-14
Ensembl D1009.4
Entrez Gene 181137 Entrez gene
SwissProt Q18915 EMBL-EBI
WormBase CE04288 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81697 Neuropeptide-like protein protein 14 [Caenorhabditis elegans] ref|NP_509508.1| Neuropeptide-Like Protein family member (nlp-14) [Caenorhabditis elegans] 1.0E-74
blast CAE57322 Hypothetical protein CBG00246 [Caenorhabditis briggsae] 3.0E-66
blast Q9NL82 Orcokinin peptides type B precursor [Contains: Orcomyotropin-like peptide; Orcokinin; Orcokinin-like peptide] dbj|BAA94754.1| orcokinin precursor [Procambarus clarkii] 2.0E-22
Sequence
Target Sequence 
>C. ELEGANS:180833_S_AT
cgacgtgcgctcgatggactagacggaagcggatttggattcgacaagagagctcttaac
tcgttggacggtgctggattcggtttcgagaagcgtgcactgaattccctggacggccaa
ggtttcggatttgagaagcgtgctttggatggtcttgatggagctggttttggatttgat
aaaagagctcttaactctttggacggggcaggcttcggattcgagaagcgagctcttgac
ggtctagatggttctggattcggttttgataaaagagccctcaactctttagacggagcc
ggatttggctttgagaaaagagctctcaactcattggacggagctggtttcggatttgag
aagcgtgctctcgacggactggacggtgctggatttggattcgacaagagagccctgaac
tctctcgacggagccggatttggattcgagaagcgtgccctcgacggtcttgacggagct
ggattcggttttgacaagcgggcgttgaactcattggacggaaatggattcggatttgac
aagaggacgtttaagcactcgtccaacaaactgcgttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACGTGCGCTCGATGGACTAGACG 283 251 73 Antisense
GAGCTCTTAACTCGTTGGACGGTGC 122 385 122 Antisense
TGGACGGTGCTGGATTCGGTTTCGA 335 547 137 Antisense
CGAGAAGCGTGCACTGAATTCCCTG 1 251 159 Antisense
TCCCTGGACGGCCAAGGTTTCGGAT 443 595 178 Antisense
TTAACTCTTTGGACGGGGCAGGCTT 210 249 263 Antisense
GAGCCCTCAACTCTTTAGACGGAGC 648 385 347 Antisense
TCTCGACGGACTGGACGGTGCTGGA 710 607 441 Antisense
AACTCTCTCGACGGAGCCGGATTTG 627 139 490 Antisense
GACGGTCTTGACGGAGCTGGATTCG 222 377 535 Antisense
AGCACTCGTCCAACAAACTGCGTTC 400 77 626 Antisense
Sequence Source Affymetrix Proprietary Database