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[180828_at]

Description ZK816.4 /REP_DB=WormBase Gene ID /WP=CE05117 /TR=Q23610 /GB=AAA82325.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 47      4grp_total > 35      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 7.7
(2)
A 		5.6
(0)
A 		2.3
(-3)
A 		8.8
(3)
A 		7
B 8.6
(3)
A 		6.6
(-0.9)
A 		8.2
(2.2)
A 		5.5
(-3)
A 		3
C 11.6
(-1.6)
A 		33.4
(3)
A 		5.1
(-3)
A 		16.5
(-0.6)
A 		28
avr 9.3
(-0.5) 		15.2
(3) 		5.2
(-3) 		10.3
(0.1) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 30.5
(3)
A 		2.3
(-3)
A 		13.2
(-0.7)
A 		10.3
(-1.3)
A 		8.4
(-1.7)
A 		7.9
(-1.8)
A 		28
B 5.9
(-0.5)
A 		9.5
(3)
A 		3.3
(-3)
A 		4.6
(-1.7)
A 		3.5
(-2.8)
A 		4.5
(-1.8)
A 		6
C 5.7
(-2.3)
A 		7.7
(-2)
A 		4
(-2.7)
A 		4.9
(-2.5)
A 		2.4
(-3)
A 		32.7
(3)
A 		30
avr 14
(2.4) 		6.5
(-2) 		6.8
(-1.8) 		6.6
(-1.9) 		4.8
(-3) 		15
(3) 		10
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 2.8
(3)
A 		1.2
(-1.4)
A 		0.6
(-3)
A 		1.3
(-1.1)
A 		2
B 2.7
(3)
A 		1.6
(-1.4)
A 		1.9
(-0.2)
A 		1.2
(-3)
A 		2
C 3.8
(-1.5)
A 		9.6
(3)
A 		1.8
(-3)
A 		2.8
(-2.2)
A 		8
avr 3.1
(0.8) 		4.1
(3) 		1.4
(-3) 		1.8
(-2.1) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 5.6
(3)
A 		0.8
(-3)
A 		2.9
(-0.4)
A 		2.6
(-0.8)
A 		2.5
(-0.9)
A 		1.9
(-1.6)
A 		5
B 2.1
(0.3)
A 		3.2
(3)
A 		0.8
(-3)
A 		1.2
(-2)
A 		1.4
(-1.5)
A 		1.7
(-0.8)
A 		2
C 2.1
(-2.2)
A 		2.4
(-2)
A 		1
(-2.9)
A 		1
(-2.9)
A 		0.8
(-3)
A 		10.9
(3)
A 		10
avr 3.3
(0.2) 		2.1
(-2.1) 		1.6
(-3) 		1.6
(-3) 		1.6
(-3) 		4.8
(3) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180828_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18259
Sequence Type Exemplar sequence
Representative Public ID ZK816.4 NCBI
Target Description ZK816.4 /REP_DB=WormBase Gene ID /WP=CE05117 /TR=Q23610 /GB=AAA82325.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076177(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076177 NCBI Caenorhabditis elegans ZK816.4 (ZK816.4) mRNA, complete cds. 11/11 None
GENEFINDER00000023573 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3349600:3350389:-1 11/11 None
ZK816.4 ENSEMBL cdna:known chromosome:CEL140:X:3349600:3350389:-1 gene:ZK816.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000023560 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3349600-3350390(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK816.4
Entrez Gene 191431 Entrez gene
SwissProt Q23610 EMBL-EBI
WormBase CE39081 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_508578 ZK816.4 [Caenorhabditis elegans] 1.0E-115
blast AAA82325 Hypothetical protein ZK816.4 [Caenorhabditis elegans] 1.0E-115
blast CAE68413 Hypothetical protein CBG14192 [Caenorhabditis briggsae] 3.0E-94
Sequence
Target Sequence 
>C. ELEGANS:180828_AT
tgtcaatttaaatttcctcctccaactcaagagtttttagatcgttctaatggattttat
gactataattctgaggaaaacttcgaaaggcccctaaatgcatcaatgtatcaacctacc
acgtgtggagaagttgagcaagaaaaagtgaaagaatgcgctgatccgctctataaaatg
ggtgccatctccgaaaactcacattacttgggatgggaagggtttattttccgcaccaaa
gcatatttttcggaagtttgcgacaatttcttcttatttgatgtctgcatcgaaccatac
aaggatgtttgctttgcagccgatagagctcgattcaactacgatgcggcgattaaaatt
ctagactttttgtgtcgggatggatatggagaaatgctcagaaacatcgagtgtttcaca
aaaacgctgacgagaagtgaaatgatgcaatgtcaagccgagctggtttcagatac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCAATTTAAATTTCCTCCTCCAA 278 559 64 Antisense
GAAAGGCCCCTAAATGCATCAATGT 419 361 148 Antisense
AATGTATCAACCTACCACGTGTGGA 192 165 168 Antisense
AAGAATGCGCTGATCCGCTCTATAA 158 153 215 Antisense
TGCCATCTCCGAAAACTCACATTAC 412 585 246 Antisense
GTTTATTTTCCGCACCAAAGCATAT 594 449 285 Antisense
AGTTTGCGACAATTTCTTCTTATTT 561 63 318 Antisense
GATGTCTGCATCGAACCATACAAGG 298 409 343 Antisense
GGATGTTTGCTTTGCAGCCGATAGA 236 513 366 Antisense
GAGCTCGATTCAACTACGATGCGGC 554 387 389 Antisense
GTCAAGCCGAGCTGGTTTCAGATAC 306 463 515 Antisense
Sequence Source Affymetrix Proprietary Database