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[180810_at]

Description R10D12.9 /REP_DB=WormBase Gene ID /WP=CE12688 /TR=P92011 /GB=CAB03252.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 71      4grp_highest_8_8 > 43      4grp_high_8_8 > 48      6grp_highest_nonorm > 63      6grp_total > 70      4grp_total > 55      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 229
1 2 3 4 range
A 209.8
(3)
P 		84.8
(-3)
P 		162.4
(0.7)
P 		125.2
(-1.1)
P 		125
B 220.9
(3)
P 		111.1
(-1.6)
P 		120.8
(-1.2)
P 		76.6
(-3)
P 		144
C 301.4
(3)
P 		72.4
(-3)
P 		155.1
(-0.8)
P 		144.8
(-1.1)
P 		229
avr 244
(3) 		89.4
(-3) 		146.1
(-0.8) 		115.5
(-2) 		155

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1050
1 2 3 4 5 6 range
A 501.8
(-1.5)
P 		384.8
(-2.4)
P 		843.4
(1.1)
P 		1085.7
(3)
P 		466.8
(-1.8)
P 		304.8
(-3)
P 		781
B 198.2
(-3)
P 		396.3
(-1.9)
P 		889.1
(0.9)
P 		1247.7
(3)
P 		696.5
(-0.2)
P 		274.7
(-2.6)
P 		1050
C 969.5
(3)
P 		328.2
(-3)
P 		473.8
(-1.6)
P 		846
(1.8)
P 		742.3
(0.9)
P 		381.2
(-2.5)
P 		641
avr 556.5
(-1.1) 		369.8
(-2.6) 		735.4
(0.4) 		1059.8
(3) 		635.2
(-0.4) 		320.2
(-3) 		740
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 76.4
(3)
P 		18.4
(-3)
P 		42.5
(-0.5)
P 		18.3
(-3)
P 		58
B 70.4
(3)
P 		27.4
(-1.8)
P 		28
(-1.7)
P 		16.3
(-3)
P 		54
C 99.8
(3)
P 		20.8
(-3)
P 		54.1
(-0.5)
P 		24.4
(-2.7)
P 		79
avr 82.2
(3) 		22.2
(-2.8) 		41.5
(-0.9) 		19.7
(-3) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 290
1 2 3 4 5 6 range
A 92.5
(-2.5)
P 		136.9
(-1.1)
P 		183.7
(0.3)
P 		275.4
(3)
P 		140.9
(-1)
P 		74.2
(-3)
P 		201
B 69
(-3)
P 		131.9
(-1.6)
P 		215.9
(0.4)
P 		330.5
(3)
P 		283
(1.9)
P 		102.4
(-2.2)
P 		262
C 359.4
(3)
P 		100.1
(-3)
P 		120.6
(-2.5)
P 		170.1
(-1.4)
P 		240.2
(0.2)
P 		127.6
(-2.4)
P 		259
avr 173.6
(-0.2) 		123
(-2.2) 		173.4
(-0.3) 		258.7
(3) 		221.4
(1.6) 		101.4
(-3) 		157
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180810_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13274
Sequence Type Exemplar sequence
Representative Public ID R10D12.9 NCBI
Target Description R10D12.9 /REP_DB=WormBase Gene ID /WP=CE12688 /TR=P92011 /GB=CAB03252.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074063(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074063 NCBI Caenorhabditis elegans R10D12.9 (R10D12.9) mRNA, complete cds. 11/11 None
R10D12.9 ENSEMBL cdna:known chromosome:CEL140:V:13956945:13959041:1 gene:R10D12.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000021054 7/11 Cross Hyb Matching Probes None
GENEFINDER00000021063 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13957990-13959041(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R10D12.9
Entrez Gene 187765 Entrez gene
SwissProt P92011 EMBL-EBI
WormBase CE12688 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03252 Hypothetical protein R10D12.9 [Caenorhabditis elegans] ref|NP_506464.1| R10D12.9 [Caenorhabditis elegans] 1.0E-128
blast CAE66183 Hypothetical protein CBG11421 [Caenorhabditis briggsae] 1.0E-68
blast NP_504349 K11D12.5 [Caenorhabditis elegans] gb|AAB94225.1| Hypothetical protein K11D12.5 [Caenorhabditis elegans] 5.0E-54
Protein Domains
Database ID Description E-Value
Pfam IPR004316 EMBL-EBI MtN3 and saliva related transmembrane protein 0.048
Pfam IPR004316 EMBL-EBI MtN3 and saliva related transmembrane protein 4.1E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506464.1 7 5-27,34-53,63-82,95-117,122-144,165-187,202-224
R10D12.9 7 5-27,34-53,63-82,95-117,122-144,165-187,202-224
Sequence
Target Sequence 
>C. ELEGANS:180810_AT
atgcggagcacacaaactcggcgatgggaacagtagcagctggagcacaaattttaggtc
ttggtggacgggtttatgaaatgagaagagccgtgaaacttggtaccactgaatacatac
ccgcattcatgcagtttgccgtatcagctcttatggctcagtggttacttttcggaattg
ttacaggaaaccaatttattgctaatgcaaatgtggcgggtttgactgcaagtgcaatta
cattgtatctctatttcaaatatccaccactcacctggacagttccacttttcaatattc
caccacaaaatgccaagaaagattatatttttgccttctgtttagaacctcctcccgttg
aaacagttttcttcttgacttgtgctggaagttttggcgatggcttcttgattggagacg
gtggaagagttctgatttttttgggtttggccttcggaagtgcatttgtgctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCGGAGCACACAAACTCGGCGAT 605 41 182 Antisense
GTACCACTGAATACATACCCGCATT 352 457 284 Antisense
TACCCGCATTCATGCAGTTTGCCGT 260 649 299 Antisense
CAGTTTGCCGTATCAGCTCTTATGG 93 207 313 Antisense
GCTCTTATGGCTCAGTGGTTACTTT 6 301 328 Antisense
TGTGGCGGGTTTGACTGCAAGTGCA 62 555 393 Antisense
CCACTTTTCAATATTCCACCACAAA 244 271 466 Antisense
TTTTGCCTTCTGTTTAGAACCTCCT 3 677 510 Antisense
AACAGTTTTCTTCTTGACTTGTGCT 226 137 543 Antisense
TTTGGCGATGGCTTCTTGATTGGAG 485 665 574 Antisense
GGCCTTCGGAAGTGCATTTGTGCTC 262 539 630 Antisense
Sequence Source Affymetrix Proprietary Database