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[180798_at]

Description T08B2.8 /REP_DB=WormBase Gene ID /WP=CE13433 /TR=Q9GYS9 /GB=AAG00016.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 53      4grp_highest_8_8 > 13      4grp_high_8_8 > 23      6grp_highest_nonorm > 75      6grp_total > 20      4grp_total > 24      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 449
1 2 3 4 range
A 909.6
(-3)
P 		1089.5
(0.3)
P 		944.1
(-2.4)
P 		1235.5
(3)
P 		326
B 969
(-3)
P 		989.6
(-2.7)
P 		1358.9
(3)
P 		1220.6
(0.9)
P 		390
C 1207.7
(3)
P 		1175.6
(0.1)
P 		1141.3
(-3)
P 		1173.7
(-0.1)
P 		66
avr 1028.8
(-3) 		1084.9
(-1.1) 		1148.1
(1) 		1209.9
(3) 		181

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 642
1 2 3 4 5 6 range
A 662.3
(-3)
P 		963.1
(0.6)
P 		1164.4
(3)
P 		998.6
(1)
P 		846
(-0.8)
P 		682.1
(-2.8)
P 		502
B 797
(-3)
P 		1093.2
(0.5)
P 		1304.7
(3)
P 		932.8
(-1.4)
P 		904.2
(-1.7)
P 		856
(-2.3)
P 		508
C 871
(-0.6)
P 		1012.9
(2.5)
P 		934.1
(0.8)
P 		1037.9
(3)
P 		759.9
(-3)
P 		1023.6
(2.7)
P 		278
avr 776.8
(-3) 		1023.1
(1.1) 		1134.4
(3) 		989.8
(0.6) 		836.7
(-2) 		853.9
(-1.7) 		358
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 219
1 2 3 4 range
A 331.2
(3)
P 		236.5
(-0.8)
P 		246.8
(-0.4)
P 		180.8
(-3)
P 		150
B 309
(2.5)
P 		244.4
(-3)
P 		314.9
(3)
P 		260.1
(-1.7)
P 		71
C 399.8
(3)
P 		338
(1.2)
P 		397.8
(2.9)
P 		197.9
(-3)
P 		202
avr 346.7
(3) 		273
(-0.3) 		319.8
(1.8) 		212.9
(-3) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 122.1
(-3)
P 		342.5
(3)
P 		253.6
(0.6)
P 		253.3
(0.6)
P 		255.3
(0.6)
P 		166.1
(-1.8)
P 		220
B 277.3
(-1.5)
P 		363.9
(2.8)
P 		316.8
(0.5)
P 		247.1
(-3)
P 		367.4
(3)
P 		319.2
(0.6)
P 		120
C 322.8
(2.1)
P 		309.1
(1.5)
P 		237.7
(-1.7)
P 		208.7
(-3)
P 		245.9
(-1.3)
P 		342.6
(3)
P 		134
avr 240.7
(-2.7) 		338.5
(3) 		269.4
(-1.1) 		236.4
(-3) 		289.5
(0.1) 		276
(-0.7) 		102
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180798_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1702
Sequence Type Exemplar sequence
Representative Public ID T08B2.8 NCBI
Target Description T08B2.8 /REP_DB=WormBase Gene ID /WP=CE13433 /TR=Q9GYS9 /GB=AAG00016.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059390(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059390 NCBI Caenorhabditis elegans T08B2.8 (T08B2.8) mRNA, complete cds. 11/11 None
SNAP00000035003 ENSEMBL cdna:SNAP chromosome:CEL140:I:6219497:6222710:-1 11/11 None
GENEFINDER00000035012 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6219497:6220019:-1 11/11 None
T08B2.8 ENSEMBL cdna:known chromosome:CEL140:I:6219416:6220020:-1 gene:T08B2.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6219425-6219948(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T08B2.8
Entrez Gene 188274 Entrez gene
SwissProt Q9GYS9 EMBL-EBI
WormBase CE13433 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_491792 phenylalanyl (F) tRNA Synthetase family member (frs-1) [Caenorhabditis elegans] gb|AAG00015.1| Phenylalanyl (f) trna synthetase protein 1, isoform a [Caenorhabditis elegans] 0.0
blast NP_871804 phenylalanyl (F) tRNA Synthetase family member (frs-1) [Caenorhabditis elegans] gb|AAO21394.1| Phenylalanyl (f) trna synthetase protein 1, isoform b [Caenorhabditis elegans] 0.0
blast CAE67141 Hypothetical protein CBG12564 [Caenorhabditis briggsae] 0.0
blast NP_491791 T08B2.8 [Caenorhabditis elegans] gb|AAG00016.1| Hypothetical protein T08B2.8 [Caenorhabditis elegans] sp|Q9GYS9|RM23_CAEEL Probable mitochondrial 39S ribosomal protein L23 (L23mt) (MRP-L23) 1.0E-89
blast Q61DA8 Probable mitochondrial 39S ribosomal protein L23 (L23mt) (MRP-L23) emb|CAE67140.1| Hypothetical protein CBG12563 [Caenorhabditis briggsae] 3.0E-80
blast EAL29787 GA12114-PA [Drosophila pseudoobscura] 2.0E-20
Protein Domains
Database ID Description E-Value
Pfam IPR002319 EMBL-EBI Phenylalanyl-tRNA synthetase, class IIc 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:180798_AT
gacaagcagattagcacgcctttggcaaccgggaaatccacaacgacgcgtctttcttcc
agatttctggatggctgtggtagaatcaccaagtgttggaaggaatcgacttccgaggaa
ttgtgttaaatttgaggttgatccgcgaatgtctcgtcatgatattcgtgaatatctcac
aaaaatttacgatttaccggttcgtgatgtgagaacagaagttcaaatgggcgatattac
atggaattcaaaactagatcatcaatacaaaaaagcaatgtggaaggatgaggataaaaa
gattgcatacgtgttcatgtcaaaaggtttcgaattctcatatccaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAAGCAGATTAGCACGCCTTTGG 380 363 15 Antisense
GCACGCCTTTGGCAACCGGGAAATC 624 295 28 Antisense
AAATCCACAACGACGCGTCTTTCTT 215 119 48 Antisense
GCGTCTTTCTTCCAGATTTCTGGAT 492 291 62 Antisense
GGATGGCTGTGGTAGAATCACCAAG 155 515 83 Antisense
GAATCGACTTCCGAGGAATTGTGTT 509 327 117 Antisense
GAGGTTGATCCGCGAATGTCTCGTC 355 403 148 Antisense
ATGTCTCGTCATGATATTCGTGAAT 230 49 163 Antisense
GATTTACCGGTTCGTGATGTGAGAA 377 427 205 Antisense
AAGATTGCATACGTGTTCATGTCAA 595 155 313 Antisense
AAGGTTTCGAATTCTCATATCCACA 162 161 338 Antisense
Sequence Source Affymetrix Proprietary Database