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[180783_at]

Description C25A8.4 /REP_DB=WormBase Gene ID /WP=CE27730 /TR=Q18143 /GB=AAB03182.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 99      4grp_total > 20      6grp_total_sub > Increase      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 71
1 2 3 4 range
A 20.7
(-1.8)
A 		7.7
(-3)
A 		18.3
(-2)
A 		73.4
(3)
A 		66
B 42.7
(1.7)
A 		53.7
(3)
A 		2.5
(-3)
A 		19.9
(-1)
A 		51
C 34.4
(-1.4)
A 		40.5
(-0.3)
A 		25.3
(-3)
A 		58.6
(3)
A 		33
avr 32.6
(-0.1) 		34
(0.2) 		15.4
(-3) 		50.6
(3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 456
1 2 3 4 5 6 range
A 22.8
(-2.7)
A 		7.6
(-3)
A 		4.1
(-3)
A 		18
(-2.8)
A 		448.3
(3)
P 		311.7
(1.2)
P 		444
B 50.1
(-2.5)
A 		39.2
(-2.6)
A 		18.5
(-2.9)
A 		9.5
(-3)
A 		458.3
(3)
P 		316.6
(1.1)
P 		449
C 2.7
(-3)
A 		49.4
(-2.3)
A 		37
(-2.5)
A 		30.1
(-2.6)
A 		405.5
(3)
P 		315.1
(1.7)
P 		403
avr 25.2
(-2.9) 		32.1
(-2.8) 		19.9
(-3) 		19.2
(-3) 		437.4
(3) 		314.5
(1.2) 		418
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 7.5
(0.9)
A 		1.7
(-3)
A 		4.8
(-0.9)
A 		10.7
(3)
A 		9
B 13.6
(3)
A 		13.3
(2.9)
A 		0.6
(-3)
A 		4.2
(-1.3)
A 		13
C 11.4
(2.6)
A 		11.6
(3)
A 		8.8
(-3)
A 		9.9
(-0.6)
A 		3
avr 10.8
(3) 		8.9
(1.1) 		4.7
(-3) 		8.3
(0.5) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 185
1 2 3 4 5 6 range
A 4.2
(-2.9)
A 		2.7
(-2.9)
A 		0.9
(-3)
A 		4.6
(-2.8)
A 		135.3
(3)
P 		75.9
(0.3)
P 		134
B 17.4
(-2.5)
A 		13.1
(-2.7)
A 		4.5
(-2.9)
A 		2.5
(-3)
A 		186.2
(3)
P 		118.1
(0.8)
P 		184
C 1
(-3)
A 		15.1
(-2.4)
A 		9.4
(-2.6)
A 		6.1
(-2.8)
A 		131.2
(3)
P 		105.5
(1.8)
P 		130
avr 7.5
(-2.9) 		10.3
(-2.8) 		4.9
(-3) 		4.4
(-3) 		150.9
(3) 		99.8
(0.9) 		147
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180783_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9781
Sequence Type Exemplar sequence
Representative Public ID C25A8.4 NCBI
Target Description C25A8.4 /REP_DB=WormBase Gene ID /WP=CE27730 /TR=Q18143 /GB=AAB03182.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068681(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068681 NCBI Caenorhabditis elegans C25A8.4 (C25A8.4) mRNA, complete cds. 11/11 None
SNAP00000010523 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7003609:7009215:1 11/11 None
C25A8.4 ENSEMBL cdna:known chromosome:CEL140:IV:7003609:7009246:1 gene:C25A8.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000010527 6/11 Cross Hyb Matching Probes None
GENEFINDER00000010530 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7003753-7009221(+) 99.08 100.0
Public Domain and Genome References
Gene Title
Ensembl C25A8.4
Entrez Gene 177464 Entrez gene
SwissProt Q18143 EMBL-EBI
WormBase CE32592 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB03182 Hypothetical protein C25A8.4 [Caenorhabditis elegans] ref|NP_501082.2| C25A8.4 [Caenorhabditis elegans] 0.0
blast CAE61784 Hypothetical protein CBG05746 [Caenorhabditis briggsae] 0.0
blast BAC06447.1 chitinase [Haemaphysalis longicornis] 8.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR001223 EMBL-EBI Glycoside hydrolase, family 18 1.3E-41
Sequence
Target Sequence 
>C. ELEGANS:180783_AT
atggtgctttcactatcatgcggtgattctgagaaatatttgaattcaattcttggtgac
aacatgacatttgcaattggcaacgtgatgaatattatggaaaaattttcccattacaca
caaaagccgtcaacgtactacagtgtatctctattgaatcgtctgtctcacattgcatta
agaatgactgataaacattccgttgatcttccattcttcttcaatcacacaaagcctgag
ttcccgtcaactgagaaatttgtgaatatttggaagaatgtgggactgaaaccggataag
ctagttttggaactctcgccatttggttggcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGTGCTTTCACTATCATGCGGTG 398 51 2419 Antisense
TTCCCATTACACACAAAAGCCGTCA 518 697 2526 Antisense
AAGCCGTCAACGTACTACAGTGTAT 396 149 2542 Antisense
GTGTATCTCTATTGAATCGTCTGTC 161 487 2561 Antisense
GAATCGTCTGTCTCACATTGCATTA 567 329 2574 Antisense
TAAACATTCCGTTGATCTTCCATTC 73 641 2610 Antisense
CCATTCTTCTTCAATCACACAAAGC 223 271 2629 Antisense
AATCACACAAAGCCTGAGTTCCCGT 438 169 2641 Antisense
GTGGGACTGAAACCGGATAAGCTAG 227 485 2698 Antisense
AAGCTAGTTTTGGAACTCTCGCCAT 487 149 2716 Antisense
GGAACTCTCGCCATTTGGTTGGCAG 662 525 2727 Antisense
Sequence Source Affymetrix Proprietary Database