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[180778_at]

Description F18C5.3 /REP_DB=WormBase Gene ID /WP=CE02652 /TR=Q19545 /GB=AAA68411.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 65      6grp_high1_10_10 > 56      4grp_highest_8_8 > 43      4grp_high_8_8 > 32      6grp_highest_nonorm > 82      6grp_total > 56      4grp_total > 47      6grp_cgc > 37      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 485
1 2 3 4 range
A 726.9
(3)
P 		403.5
(-1.5)
P 		296.1
(-3)
P 		349.3
(-2.3)
P 		431
B 597.8
(3)
P 		403.8
(-1.2)
P 		403
(-1.2)
P 		322.3
(-3)
P 		276
C 749.1
(3)
P 		264
(-3)
P 		467.6
(-0.5)
P 		454
(-0.6)
P 		485
avr 691.3
(3) 		357.1
(-3) 		388.9
(-2.4) 		375.2
(-2.7) 		334

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 398
1 2 3 4 5 6 range
A 345.2
(-0.1)
P 		361.1
(0.5)
P 		356.4
(0.3)
P 		266.2
(-3)
P 		352
(0.1)
P 		430.7
(3)
P 		165
B 479.2
(0.6)
P 		438.7
(-0)
P 		438.3
(-0)
P 		407.4
(-0.5)
P 		240.2
(-3)
P 		638.5
(3)
P 		398
C 347.9
(-1.4)
P 		506.5
(1)
P 		370.7
(-1)
P 		309.7
(-2)
P 		241.7
(-3)
P 		636.4
(3)
P 		395
avr 390.8
(-0.7) 		435.4
(0.3) 		388.5
(-0.7) 		327.8
(-2) 		278
(-3) 		568.5
(3) 		291
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 264.7
(3)
P 		87.6
(-2)
P 		77.4
(-2.3)
P 		51.1
(-3)
P 		214
B 190.6
(3)
P 		99.7
(-1.5)
P 		93.4
(-1.8)
P 		68.7
(-3)
P 		122
C 248
(3)
P 		75.9
(-3)
P 		163
(0)
P 		76.5
(-3)
P 		172
avr 234.4
(3) 		87.7
(-2.2) 		111.3
(-1.4) 		65.4
(-3) 		169

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 176
1 2 3 4 5 6 range
A 63.6
(-3)
P 		128.4
(3)
P 		77.6
(-1.7)
P 		67.5
(-2.6)
P 		106.2
(0.9)
P 		104.9
(0.8)
P 		65
B 166.7
(-0)
P 		146
(-0.9)
P 		106.4
(-2.6)
P 		107.9
(-2.6)
P 		97.6
(-3)
P 		238.1
(3)
P 		141
C 129
(-0.3)
P 		154.5
(0.7)
P 		94.3
(-1.7)
P 		62.3
(-3)
P 		78.2
(-2.4)
P 		213
(3)
P 		151
avr 119.8
(-0.7) 		143
(0.6) 		92.8
(-2.2) 		79.2
(-3) 		94
(-2.2) 		185.3
(3) 		106
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180778_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4059
Sequence Type Exemplar sequence
Representative Public ID F18C5.3 NCBI
Target Description F18C5.3 /REP_DB=WormBase Gene ID /WP=CE02652 /TR=Q19545 /GB=AAA68411.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062922(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062922 NCBI Caenorhabditis elegans F18C5.3 (F18C5.3) mRNA, complete cds. 11/11 None
SNAP00000016656 ENSEMBL cdna:SNAP chromosome:CEL140:II:6549488:6554616:1 11/11 None
GENEFINDER00000016668 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6546795:6554616:1 11/11 None
F18C5.3 ENSEMBL cdna:known chromosome:CEL140:II:6546795:6554791:1 gene:F18C5.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6546791-6554613(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F18C5.3
Entrez Gene 174080 Entrez gene
SwissProt Q19545 EMBL-EBI
WormBase CE02652 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1589525_AT similar to Down-regulated in metastasis protein (Key-1A6 protein) (Novel nucleolar protein 73) (NNP73) cfa
CANINE_2:CFA.15590.1.A1_AT similar to Down-regulated in metastasis protein (Key-1A6 protein) (Novel nucleolar protein 73) (NNP73) cfa
CANINE_2:CFA.15590.1.A1_S_AT similar to Down-regulated in metastasis protein (Key-1A6 protein) (Novel nucleolar protein 73) (NNP73) cfa
CANINE_2:CFAAFFX.11342.1.S1_AT similar to Down-regulated in metastasis protein (Key-1A6 protein) (Novel nucleolar protein 73) (NNP73) cfa
DROSOPHILA_2:1630247_AT dm
DROSGENOME1:153124_AT dm
CHICKEN:GGA.5499.2.S1_A_AT similar to down-regulated in metastasis gga
CHICKEN:GGAAFFX.7408.1.S1_AT similar to down-regulated in metastasis gga
CHICKEN:GGAAFFX.7409.1.S1_AT similar to down-regulated in metastasis gga
HG-U133A_2:209725_AT down-regulated in metastasis hs
HG-U133A:209725_AT down-regulated in metastasis hs
HG-U133_PLUS_2:209725_AT down-regulated in metastasis hs
HG-FOCUS:209725_AT down-regulated in metastasis hs
HU35KSUBA:AA358888_AT down-regulated in metastasis hs
U133_X3P:G11464475_3P_AT down-regulated in metastasis hs
HG-U95AV2:38560_AT down-regulated in metastasis hs
HG-U95AV2:38561_AT Down-regulated in metastasis hs
HG-U95AV2:38562_G_AT Down-regulated in metastasis hs
HG-U95D:91881_S_AT Down-regulated in metastasis hs
HG-U95D:91883_R_AT Down-regulated in metastasis hs
HU35KSUBD:RC_AA197333_AT Down-regulated in metastasis hs
MOE430A:1452252_AT RIKEN cDNA 3830408P06 gene mm
MOUSE430A_2:1452252_AT RIKEN cDNA 3830408P06 gene mm
MOUSE430_2:1452252_AT RIKEN cDNA 3830408P06 gene mm
MU11KSUBA:AA617408_RC_S_AT RIKEN cDNA 3830408P06 gene mm
MG-U74AV2:104463_AT RIKEN cDNA 3830408P06 gene mm
MOE430B:1459028_AT RIKEN cDNA 3830408P06 gene, mRNA (cDNA clone IMAGE:3494784) mm
MOUSE430_2:1459028_AT RIKEN cDNA 3830408P06 gene, mRNA (cDNA clone IMAGE:3494784) mm
MU19KSUBA:TC25529_AT RIKEN cDNA 3830408P06 gene, mRNA (cDNA clone IMAGE:3494784) mm
YEAST_2:1776909_AT Possible snoRNA-binding protein, based on computational analysis of large-scale protein-protein interaction data Sc
Protein Similarities
Method ID Description E-Value
blast AAA68411 Hypothetical protein F18C5.3 [Caenorhabditis elegans] ref|NP_495323.1| F18C5.3 [Caenorhabditis elegans] 0.0
blast CAE59343 Hypothetical protein CBG02688 [Caenorhabditis briggsae] 0.0
blast XP_532665 PREDICTED: similar to Down-regulated in metastasis protein (Key-1A6 protein) (Novel nucleolar protein 73) (NNP73) [Canis familiaris] 1.0E-106
blast XP_587627 PREDICTED: similar to Down-regulated in metastasis protein (Key-1A6 protein) (Novel nucleolar protein 73) (NNP73) [Bos taurus] 1.0E-105
Sequence
Target Sequence 
>C. ELEGANS:180778_AT
gacgtgtgcttctggatgagccgacatcttcgacacgagaaaatggacacggcaattgga
gagcaagcatctaagaacattgtttgtctggcgaaagtgattgatattgaagactataaa
actcttgttggatacattgcagcggcttgtcgatatgaaatcaagcatcacgttaaacaa
tctttaaagagaatcaactgtttcaaattagttgctgcattattcgtcactggagacgac
gaaaggaatattatagttctcgacgcattcatgtcacttcttgtcagagaactaaaaact
tcaagtgacgaagaactttccaaactaacccaagaagtgtgcggtcttgtgaagaagaag
attggagaggacgcctacagtaatcgtgtcgcaacgtgccaaaaagtggctgccgagaag
attactgaccgaaaaagaaagattcgggagctggctgtaactgcacctgaagatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGTGTGCTTCTGGATGAGCCGAC 344 377 3874 Antisense
TGGATGAGCCGACATCTTCGACACG 689 549 3886 Antisense
GGATACATTGCAGCGGCTTGTCGAT 412 511 4003 Antisense
AGTTGCTGCATTATTCGTCACTGGA 33 65 4083 Antisense
ATTATAGTTCTCGACGCATTCATGT 52 15 4123 Antisense
GCATTCATGTCACTTCTTGTCAGAG 69 309 4138 Antisense
GGACGCCTACAGTAATCGTGTCGCA 32 525 4242 Antisense
GTAATCGTGTCGCAACGTGCCAAAA 569 333 4253 Antisense
GTGGCTGCCGAGAAGATTACTGACC 166 489 4279 Antisense
AGAAAGATTCGGGAGCTGGCTGTAA 434 71 4309 Antisense
CTGGCTGTAACTGCACCTGAAGATG 650 241 4324 Antisense
Sequence Source Affymetrix Proprietary Database