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[180747_s_at]

Description K02D7.4 /REP_DB=WormBase Gene ID /WP=CE17997 /TR=O45104 /GB=AAC02607.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 03      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 20      6grp_total > 24      4grp_total > 60      6grp_highest_sub > De      6grp_cgc > 33      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1506
1 2 3 4 range
A 1766
(2.4)
P 		1287
(0)
P 		1879.2
(3)
P 		694.1
(-3)
P 		1185
B 1352.9
(0)
P 		1450.4
(0.5)
P 		2022.6
(3)
P 		674.2
(-3)
P 		1348
C 1716.5
(2.3)
P 		1646.8
(2)
P 		1878.9
(3)
P 		516.5
(-3)
P 		1362
avr 1611.8
(1.5) 		1461.4
(0.8) 		1926.9
(3) 		628.3
(-3) 		1299

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 898
1 2 3 4 5 6 range
A 1465.8
(3)
P 		1427.3
(2.7)
P 		949.3
(-1.3)
P 		748.4
(-3)
P 		758.5
(-2.9)
P 		761.7
(-2.9)
P 		717
B 1536.8
(3)
P 		1175.5
(0.6)
P 		1205.7
(0.8)
P 		872.9
(-1.4)
P 		685.9
(-2.7)
P 		638.7
(-3)
P 		898
C 1293
(2)
P 		1413.6
(3)
P 		1057.2
(-0.1)
P 		736.4
(-2.9)
P 		857.6
(-1.8)
P 		723.4
(-3)
P 		690
avr 1431.9
(3) 		1338.8
(2.2) 		1070.7
(0) 		785.9
(-2.4) 		767.3
(-2.5) 		707.9
(-3) 		724
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 568
1 2 3 4 range
A 643
(3)
P 		279.4
(-1)
P 		491.3
(1.3)
P 		101.6
(-3)
P 		541
B 431.4
(2.3)
P 		358.2
(1)
P 		468.7
(3)
P 		143.7
(-3)
P 		325
C 568.2
(2.1)
P 		473.5
(1.1)
P 		654.8
(3)
P 		87.1
(-3)
P 		568
avr 547.5
(3) 		370.4
(0.6) 		538.3
(2.9) 		110.8
(-3) 		437

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 387
1 2 3 4 5 6 range
A 270.2
(-1.4)
P 		507.6
(3)
P 		206.7
(-2.6)
P 		189.8
(-2.9)
P 		228.9
(-2.2)
P 		185.5
(-3)
P 		322
B 534.7
(3)
P 		391.3
(0.2)
P 		292.8
(-1.8)
P 		231.2
(-3)
P 		278.7
(-2.1)
P 		238.2
(-2.9)
P 		304
C 479.2
(3)
P 		431.3
(2.1)
P 		269.1
(-0.8)
P 		148.1
(-3)
P 		277.5
(-0.7)
P 		242.2
(-1.3)
P 		331
avr 428
(2.6) 		443.4
(3) 		256.2
(-1.4) 		189.7
(-3) 		261.7
(-1.3) 		222
(-2.2) 		254
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180747_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10747
Sequence Type Exemplar sequence
Representative Public ID K02D7.4 NCBI
Target Description K02D7.4 /REP_DB=WormBase Gene ID /WP=CE17997 /TR=O45104 /GB=AAC02607.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067502(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067502 NCBI Caenorhabditis elegans Defecation Suppressor of Clk-1 family member (dsc-4) (dsc-4) mRNA, complete cds. 11/11 None
SNAP00000015229 ENSEMBL cdna:SNAP chromosome:CEL140:IV:315119:320470:-1 11/11 None
GENEFINDER00000015236 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:311552:322584:-1 11/11 None
K02D7.4.2 ENSEMBL cdna:known chromosome:CEL140:IV:314831:320563:-1 gene:K02D7.4 11/11 None
K02D7.4.1 ENSEMBL cdna:known chromosome:CEL140:IV:315051:320619:-1 gene:K02D7.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:315118-321198(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dsc-4
Ensembl K02D7.4
Entrez Gene 176852 Entrez gene
SwissProt O45104 EMBL-EBI
WormBase CE33667 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC02607 Defecation suppressor of clk-1 protein 4 [Caenorhabditis elegans] ref|NP_499903.3| Defecation Suppressor of Clk-1 family member (dsc-4) [Caenorhabditis elegans] gb|AAR27937.1| microsomal triglyceride transfer protein [Caenorhabditis elegans] 0.0
blast CAE67922 Hypothetical protein CBG13520 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 9.4E-9
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 1.4E-6
Pfam IPR007087 EMBL-EBI Zn-finger, C2H2 type 1.8E-6
Sequence
Target Sequence 
>C. ELEGANS:180747_S_AT
gatcagcttcttcaagatccggcgctccacaagggtctgttcgaattggagttgctggtc
acaagctaccaactcaccacatcttcaagggaagtactgacctgctttccactgtctggg
aagcagatggaaggacgcataaggcatttgaaggtcatgttcctgttagagacgttcgac
tatcggtgccattgctctctggattgactcttgacgttgatagcgttggagcaattagta
tgagagttcttgcatcggcggaagtttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCAGCTTCTTCAAGATCCGGCGC 234 421 2435 Antisense
GCTCCACAAGGGTCTGTTCGAATTG 424 297 2458 Antisense
GTTGCTGGTCACAAGCTACCAACTC 294 437 2485 Antisense
AACTCACCACATCTTCAAGGGAAGT 694 139 2505 Antisense
AGGGAAGTACTGACCTGCTTTCCAC 138 59 2522 Antisense
CCTGCTTTCCACTGTCTGGGAAGCA 125 267 2535 Antisense
TCATGTTCCTGTTAGAGACGTTCGA 46 621 2589 Antisense
AGAGACGTTCGACTATCGGTGCCAT 462 65 2602 Antisense
GCCATTGCTCTCTGGATTGACTCTT 524 281 2622 Antisense
GGATTGACTCTTGACGTTGATAGCG 640 507 2635 Antisense
GAGTTCTTGCATCGGCGGAAGTTTC 701 397 2678 Antisense
Sequence Source Affymetrix Proprietary Database