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[180731_at]

Description B0524.6 /REP_DB=WormBase Gene ID /WP=CE20468 /TR=Q9N5Z6 /GB=AAF39745.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 58      4grp_total > 0      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 27.2
(-2.8)
A 		26.2
(-3)
A 		38.4
(-0.5)
A 		55.1
(3)
A 		29
B 12.7
(-2.1)
A 		6.4
(-3)
A 		48
(3)
A 		46.9
(2.8)
A 		42
C 43.8
(-0.5)
A 		27.9
(-1.6)
A 		6.8
(-3)
A 		95.9
(3)
P 		89
avr 27.9
(-2) 		20.2
(-3) 		31.1
(-1.6) 		66
(3) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 30.5
(2.6)
A 		21.1
(0.2)
A 		9.5
(-2.7)
A 		25.3
(1.3)
A 		8.2
(-3)
A 		32.1
(3)
A 		24
B 19.4
(0.3)
A 		9.5
(-2.6)
A 		28
(2.8)
A 		8
(-3)
A 		21.6
(0.9)
A 		28.8
(3)
A 		21
C 31.4
(-1)
A 		32.3
(-0.7)
A 		25.8
(-3)
A 		42.9
(3)
A 		29.1
(-1.8)
A 		26.5
(-2.8)
A 		17
avr 27.1
(1.7) 		21
(-2.1) 		21.1
(-2.1) 		25.4
(0.7) 		19.6
(-3) 		29.1
(3) 		10
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 9.9
(2.7)
A 		5.7
(-3)
A 		10.1
(3)
A 		8.1
(0.3)
A 		4
B 4
(-1.5)
A 		1.6
(-3)
A 		11.1
(3)
A 		10
(2.3)
A 		10
C 14.5
(2.3)
A 		8
(-0.6)
A 		2.4
(-3)
A 		16.2
(3)
P 		14
avr 9.5
(1.2) 		5.1
(-3) 		7.9
(-0.3) 		11.4
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 5.6
(0.7)
A 		7.5
(2.7)
A 		2.1
(-3)
A 		6.4
(1.5)
A 		2.5
(-2.6)
A 		7.8
(3)
A 		6
B 6.8
(0.3)
A 		3.2
(-2.2)
A 		6.8
(0.3)
A 		2.1
(-3)
A 		8.8
(1.7)
A 		10.7
(3)
A 		9
C 11.6
(3)
A 		9.8
(0.8)
A 		6.6
(-3)
A 		8.6
(-0.6)
A 		9.4
(0.4)
A 		8.9
(-0.2)
A 		5
avr 8
(1.3) 		6.8
(-0.5) 		5.2
(-3) 		5.7
(-2.2) 		6.9
(-0.4) 		9.1
(3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180731_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8185
Sequence Type Exemplar sequence
Representative Public ID B0524.6 NCBI
Target Description B0524.6 /REP_DB=WormBase Gene ID /WP=CE20468 /TR=Q9N5Z6 /GB=AAF39745.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065074(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065074 NCBI Caenorhabditis elegans B0524.6 (B0524.6) mRNA, complete cds. 11/11 None
SNAP00000041792 ENSEMBL cdna:SNAP chromosome:CEL140:III:1891395:1915794:-1 10/11 None
B0524.6 ENSEMBL cdna:known chromosome:CEL140:III:1902182:1905624:-1 gene:B0524.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000041797 6/11 Cross Hyb Matching Probes None
GENEFINDER00000041795 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1902181-1905624(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0524.6
Entrez Gene 182027 Entrez gene
SwissProt Q9N5Z6 EMBL-EBI
WormBase CE20468 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39745 Hypothetical protein B0524.6 [Caenorhabditis elegans] ref|NP_497475.1| B0524.6 [Caenorhabditis elegans] 1.0E-133
blast AAF39745 Hypothetical protein B0524.6 [Caenorhabditis elegans] ref|NP_497475.1| B0524.6 [Caenorhabditis elegans] 1.0E-124
blast AAF39750 Hypothetical protein B0524.5 [Caenorhabditis elegans] ref|NP_497473.1| B0524.5 [Caenorhabditis elegans] 8.0E-49
blast CAE66467 Hypothetical protein CBG11744 [Caenorhabditis briggsae] 1.0E-33
blast CAE66467 Hypothetical protein CBG11744 [Caenorhabditis briggsae] 3.0E-33
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000041792 1 323-345
Sequence
Target Sequence 
>C. ELEGANS:180731_AT
acgccggcgcagcgggagaaaatgatgaaaaatggggaaaaggagaatgtttcgcaaaaa
caagcagcagtgtcgatggaaactcgtaaaaattgtaacgctatagcaaaggttttggac
attaaaccgtcgacaacaccgacaaattcatatgcagaaagattgaaaattcgaattaaa
gcatatcataatgagggattcattttggcgtcccgggaaggccctccaacacttccatca
cgacttccacccaccgcagcagagccaaaaatctcacttccgacatcaaaaccaccgcca
attcccataaaaatcgaggaaatggttcaaaaaaccgtccaacaccttcgaaaaccccaa
aaacacgccagaatcgtcgtaaaaccgataaaatgtgcccaaaaaccacaaaattcggcg
gaaaaaggacgaaatcccgttaaaaaatcaattgaaccggttgaaccaatggttttggag
ccaattgctccaccaccaccacagtctcctgtaataagttacagtgaagcgtgtaaaatg
tactatcagacactgtcacaaatgacgcttttggctgattttgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGCCGGCGCAGCGGGAGAAAATGA 132 91 208 Antisense
GCAGCAGTGTCGATGGAAACTCGTA 323 311 271 Antisense
TTTGGACATTAAACCGTCGACAACA 704 663 321 Antisense
TGAGGGATTCATTTTGGCGTCCCGG 511 567 399 Antisense
GCAGAGCCAAAAATCTCACTTCCGA 11 311 466 Antisense
AAATCTCACTTCCGACATCAAAACC 146 119 476 Antisense
CACCGCCAATTCCCATAAAAATCGA 330 201 500 Antisense
ACACGCCAGAATCGTCGTAAAACCG 85 109 570 Antisense
CCACCACAGTCTCCTGTAATAAGTT 634 273 703 Antisense
AATGTACTATCAGACACTGTCACAA 651 163 744 Antisense
AAATGACGCTTTTGGCTGATTTTGC 664 117 767 Antisense
Sequence Source Affymetrix Proprietary Database