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[180730_at]

Description F31E8.5 /REP_DB=WormBase Gene ID /WP=CE01932 /TR=Q19938 /GB=AAA98022.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 88      4grp_high_8_8 > 21      6grp_total > 98      4grp_total > 05      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 42.1
(3)
A 		33.5
(-3)
A 		35.1
(-1.9)
A 		36.1
(-1.2)
A 		9
B 53.6
(1.6)
A 		30.5
(-3)
A 		60.4
(3)
P 		47.3
(0.4)
P 		30
C 53.2
(-1.6)
P 		58.3
(-1.2)
P 		35.9
(-3)
P 		108.6
(3)
P 		73
avr 49.6
(-0.7) 		40.8
(-3) 		43.8
(-2.2) 		64
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 218
1 2 3 4 5 6 range
A 56.5
(-2.8)
A 		49.5
(-3)
A 		56.3
(-2.8)
P 		52.5
(-2.9)
P 		160.7
(0.3)
P 		253.5
(3)
P 		204
B 48.2
(-3)
P 		53.4
(-2.8)
P 		60.3
(-2.5)
A 		61.7
(-2.4)
P 		151.2
(1.2)
P 		194.5
(3)
P 		146
C 38.4
(-2.9)
P 		73.1
(-1.5)
P 		35.1
(-3)
A 		58.1
(-2.1)
M 		164.9
(2)
P 		192.3
(3)
P 		157
avr 47.7
(-3) 		58.7
(-2.6) 		50.6
(-2.9) 		57.4
(-2.6) 		158.9
(1) 		213.4
(3) 		166
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 15.3
(3)
A 		7.3
(-1.8)
A 		9.2
(-0.7)
A 		5.3
(-3)
A 		10
B 17.1
(3)
A 		7.5
(-3)
A 		14
(1.1)
P 		10.1
(-1.4)
P 		10
C 17.6
(2.3)
P 		16.8
(1.4)
P 		12.5
(-3)
P 		18.3
(3)
P 		6
avr 16.7
(3) 		10.5
(-3) 		11.9
(-1.6) 		11.2
(-2.3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 10.4
(-3)
A 		17.6
(-2.2)
A 		12.3
(-2.8)
P 		13.3
(-2.7)
P 		48.5
(1.5)
P 		61.7
(3)
P 		51
B 16.8
(-2.8)
P 		17.8
(-2.7)
P 		14.6
(-3)
A 		16.3
(-2.8)
P 		61.4
(1.8)
P 		72.5
(3)
P 		58
C 14.2
(-2.4)
P 		22.3
(-1.6)
P 		8.9
(-3)
A 		11.7
(-2.7)
M 		53.4
(1.8)
P 		64.4
(3)
P 		56
avr 13.8
(-2.8) 		19.2
(-2.2) 		11.9
(-3) 		13.8
(-2.8) 		54.4
(1.7) 		66.2
(3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180730_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4904
Sequence Type Exemplar sequence
Representative Public ID F31E8.5 NCBI
Target Description F31E8.5 /REP_DB=WormBase Gene ID /WP=CE01932 /TR=Q19938 /GB=AAA98022.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062992(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062992 NCBI Caenorhabditis elegans F31E8.5 (F31E8.5) mRNA, complete cds. 11/11 None
SNAP00000019076 ENSEMBL cdna:SNAP chromosome:CEL140:II:6804995:6807661:-1 11/11 None
GENEFINDER00000019088 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6804995:6807136:-1 11/11 None
F31E8.5 ENSEMBL cdna:known chromosome:CEL140:II:6804995:6807175:-1 gene:F31E8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6804989-6807195(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F31E8.5
Entrez Gene 174119 Entrez gene
SwissProt Q19938 EMBL-EBI
WormBase CE35516 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495393 F31E8.5 [Caenorhabditis elegans] gb|AAA98022.2| Hypothetical protein F31E8.5 [Caenorhabditis elegans] 0.0
blast CAE65974 Hypothetical protein CBG11159 [Caenorhabditis briggsae] 1.0E-117
blast CAE65974 Hypothetical protein CBG11159 [Caenorhabditis briggsae] 1.0E-112
blast CAA21643 Hypothetical protein Y43F8A.2 [Caenorhabditis elegans] ref|NP_507770.1| Y43F8A.2 [Caenorhabditis elegans] 3.0E-61
blast CAA21643 Hypothetical protein Y43F8A.2 [Caenorhabditis elegans] ref|NP_507770.1| Y43F8A.2 [Caenorhabditis elegans] 4.0E-61
Sequence
Target Sequence 
>C. ELEGANS:180730_AT
gagagtttcggtcaacacgtatcctgttcatattccaaacgatcagcacgtttccaatgc
ttacaaaatgttcactctatcgacgaataagtgccactggttcagactttggatcaccca
gttaccaattgaccaatacaaaacaggaatgaaactttccgagactgtcgatttctttct
caacgaaggattttacgtctctcctcagtctgatgttgcttatccattgccgggtgtgag
tgagctcacaaagttcaaattcactgtctcacgagaaccaagtaacgaaaactacgttga
catggtgttcggagaaatgcaaggaaatccaatctatcaggatcaattcaagcttccagt
tacagagagcacatcattcaatattcatgttatcaaggcacctggttgtgatattgaaat
ggatacgcgtactttgttgtggaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAGTTTCGGTCAACACGTATCCT 90 395 882 Antisense
ACACGTATCCTGTTCATATTCCAAA 596 109 896 Antisense
GATCAGCACGTTTCCAATGCTTACA 327 421 922 Antisense
GTGCCACTGGTTCAGACTTTGGATC 50 479 972 Antisense
GGATCACCCAGTTACCAATTGACCA 509 511 992 Antisense
GAAACTTTCCGAGACTGTCGATTTC 69 357 1032 Antisense
CGAAGGATTTTACGTCTCTCCTCAG 574 253 1065 Antisense
CTCCTCAGTCTGATGTTGCTTATCC 447 235 1082 Antisense
GCTTATCCATTGCCGGGTGTGAGTG 53 305 1099 Antisense
GATCAATTCAAGCTTCCAGTTACAG 504 421 1222 Antisense
GGATACGCGTACTTTGTTGTGGAAT 21 511 1302 Antisense
Sequence Source Affymetrix Proprietary Database