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[180729_s_at]

Description F08B6.5 /REP_DB=WormBase Gene ID /WP=CE20659 /TR=Q9TYS5 /GB=AAC78233.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 09      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 72      6grp_total > 19      4grp_total > 46      6grp_cgc > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 276
1 2 3 4 range
A 501.4
(1.6)
P 		538.3
(3)
P 		377
(-3)
P 		474.4
(0.6)
P 		161
B 524.9
(3)
P 		358.1
(-1.6)
P 		320
(-2.7)
P 		308.9
(-3)
P 		216
C 585.3
(3)
P 		407.4
(-2)
P 		446.7
(-0.9)
P 		373.8
(-3)
P 		212
avr 537.2
(3) 		434.6
(-0.9) 		381.2
(-3) 		385.7
(-2.8) 		156

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 238
1 2 3 4 5 6 range
A 397.2
(0.8)
P 		463.9
(3)
P 		279.9
(-3)
P 		450
(2.5)
P 		421.2
(1.6)
P 		434.1
(2)
P 		184
B 424.1
(3)
P 		389
(0.4)
P 		347
(-2.7)
P 		381.3
(-0.2)
P 		378.7
(-0.4)
P 		343.5
(-3)
P 		81
C 517.6
(3)
P 		376.6
(-1.9)
P 		459.5
(1)
P 		433.3
(0.1)
P 		466.6
(1.2)
P 		345.9
(-3)
P 		172
avr 446.3
(3) 		409.8
(0.4) 		362.1
(-3) 		421.5
(1.2) 		422.2
(1.3) 		374.5
(-2.1) 		84
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 131
1 2 3 4 range
A 182.6
(3)
P 		116.9
(-0.5)
P 		98.6
(-1.5)
P 		69.4
(-3)
P 		113
B 167.4
(3)
P 		88.4
(-1.7)
P 		74.2
(-2.5)
P 		65.8
(-3)
P 		102
C 193.7
(3)
P 		117.1
(-0.5)
P 		155.7
(1.3)
P 		63
(-3)
P 		131
avr 181.2
(3) 		107.5
(-0.8) 		109.5
(-0.7) 		66.1
(-3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 73.2
(-2.3)
P 		165
(3)
P 		61
(-3)
P 		114.1
(0.1)
P 		127.1
(0.8)
P 		105.7
(-0.4)
P 		104
B 147.6
(2.5)
P 		129.5
(0.9)
P 		84.3
(-3)
P 		101
(-1.6)
P 		153.8
(3)
P 		128.1
(0.8)
P 		70
C 191.9
(3)
P 		114.9
(-1.4)
P 		116.9
(-1.3)
P 		87.1
(-3)
P 		151
(0.7)
P 		115.8
(-1.4)
P 		105
avr 137.6
(2.3) 		136.5
(2.2) 		87.4
(-3) 		100.7
(-1.6) 		144
(3) 		116.5
(0.1) 		57
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180729_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.566
Sequence Type Exemplar sequence
Representative Public ID F08B6.5 NCBI
Target Description F08B6.5 /REP_DB=WormBase Gene ID /WP=CE20659 /TR=Q9TYS5 /GB=AAC78233.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025858(11), NM_059539(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025858 NCBI Caenorhabditis elegans Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-21) (pqn-21) mRNA, complete cds. 11/11 None
NM_059539 NCBI Caenorhabditis elegans Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-21) (pqn-21) mRNA, complete cds. 11/11 A 
SNAP00000014175 ENSEMBL cdna:SNAP chromosome:CEL140:I:6782650:6788040:-1 11/11 None
GENEFINDER00000014185 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6782694:6788040:-1 11/11 None
C37A2.5b ENSEMBL cdna:known chromosome:CEL140:I:6782110:6788040:-1 gene:C37A2.5 11/11 None
C37A2.5a ENSEMBL cdna:known chromosome:CEL140:I:6782110:6788044:-1 gene:C37A2.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6782578-6783555(-) 95.36 100.0
Public Domain and Genome References
Ensembl C37A2.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68236 Prion-like-(q/n-rich)-domain-bearing protein protein 21, isoform a [Caenorhabditis elegans] ref|NP_001021029.1| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-21) [Caenorhabditis elegans] gb|AAF89696.1| putative MAP-like protein [Caenorhabditis elegans] 0.0
blast AAN84846 Prion-like-(q/n-rich)-domain-bearing protein protein 21, isoform b [Caenorhabditis elegans] ref|NP_491940.2| Prion-like-(Q/N-rich)-domain-bearing protein family member (pqn-21) [Caenorhabditis elegans] 0.0
Sequence
Target Sequence 
>C. ELEGANS:180729_S_AT
agcccaaccaccaacaatgattcaagctcatccaatggcaggcgtctttattcctcctcc
atttcatccagcaatgatgaatccacaaatggctgcaatgatggatcagcaacgaattgc
tgttgcgatgtcacaacaacaacaaatgtcacggacaccgtctcgacaaaccatatccgt
atcagattcccacaagatccatgaactggcgaaatctcgcgagaaccagcaacgacaaca
gtcggctacaccaaagacgccatctgaaagtgtgagtactactggagtagtcacaccgca
acgacaattccttcctccacagatgatgcagccaggaacgccgcaaagtattctattaca
acagcagcaacaacagcaacagcaacagcaacaagcggctgcacaacatcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCCCAACCACCAACAATGATTCAA 119 83 252 Antisense
AAGCTCATCCAATGGCAGGCGTCTT 102 151 275 Antisense
CTCCTCCATTTCATCCAGCAATGAT 473 235 305 Antisense
AAATGTCACGGACACCGTCTCGACA 471 115 395 Antisense
GTCTCGACAAACCATATCCGTATCA 213 471 411 Antisense
ATCCGTATCAGATTCCCACAAGATC 694 37 426 Antisense
AAATCTCGCGAGAACCAGCAACGAC 161 119 463 Antisense
GTCGGCTACACCAAAGACGCCATCT 174 471 492 Antisense
TACTGGAGTAGTCACACCGCAACGA 45 645 531 Antisense
CAGATGATGCAGCCAGGAACGCCGC 134 207 571 Antisense
GCAACAAGCGGCTGCACAACATCAA 223 319 639 Antisense
Sequence Source Affymetrix Proprietary Database