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[180727_at]

Description F49F1.5 /REP_DB=WormBase Gene ID /WP=CE17088 /TR=Q9GZE4 /GB=AAF99969.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 57      6grp_high1_10_10 > 75      4grp_high_8_8 > 53      6grp_highest_nonorm > 85      6grp_total > 65      4grp_total > 51      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1494
1 2 3 4 range
A 671.8
(-0.6)
P 		1562.5
(3)
P 		1179.6
(1.4)
P 		84.6
(-3)
A 		1478
B 613
(-0.2)
P 		1235.9
(3)
P 		974.8
(1.7)
P 		68.7
(-3)
A 		1167
C 513.5
(-1)
P 		1179.3
(3)
P 		964.5
(1.7)
P 		178.4
(-3)
P 		1001
avr 599.4
(-0.6) 		1325.9
(3) 		1039.6
(1.6) 		110.6
(-3) 		1215

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 582
1 2 3 4 5 6 range
A 505
(-3)
P 		653.6
(-0.8)
P 		833.4
(1.8)
P 		905.2
(2.9)
P 		586.5
(-1.8)
P 		911.4
(3)
P 		406
B 500.3
(-3)
P 		729
(0.2)
P 		935.6
(3)
P 		593.2
(-1.7)
P 		637.1
(-1.1)
P 		913.3
(2.7)
P 		435
C 619.4
(-3)
P 		697.6
(-2)
P 		779.5
(-0.9)
P 		837.4
(-0.2)
P 		752.6
(-1.3)
P 		1082.6
(3)
P 		463
avr 541.6
(-3) 		693.4
(-0.9) 		849.5
(1.3) 		778.6
(0.3) 		658.7
(-1.4) 		969.1
(3) 		428
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 327
1 2 3 4 range
A 244.6
(1.3)
P 		339.2
(3)
P 		308.4
(2.4)
P 		12.4
(-3)
A 		327
B 195.5
(0.7)
P 		305.2
(3)
P 		225.9
(1.4)
P 		14.6
(-3)
A 		291
C 170
(-0.3)
P 		339.1
(3)
P 		336.2
(2.9)
P 		30.1
(-3)
P 		309
avr 203.4
(0.6) 		327.8
(3) 		290.2
(2.3) 		19
(-3) 		309

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 269
1 2 3 4 5 6 range
A 93.1
(-3)
P 		232.4
(3)
P 		181.5
(0.8)
P 		229.6
(2.9)
P 		177
(0.6)
P 		221.9
(2.5)
P 		139
B 174.1
(-2.4)
P 		242.7
(-0.2)
P 		227.2
(-0.7)
P 		157.1
(-3)
P 		258.9
(0.3)
P 		340.6
(3)
P 		184
C 229.6
(-1.1)
P 		212.9
(-1.6)
P 		198.4
(-2.1)
P 		168.4
(-3)
P 		243.5
(-0.7)
P 		362.4
(3)
P 		194
avr 165.6
(-3) 		229.3
(-0.3) 		202.4
(-1.5) 		185
(-2.2) 		226.5
(-0.4) 		308.3
(3) 		143
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180727_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11075
Sequence Type Exemplar sequence
Representative Public ID F49F1.5 NCBI
Target Description F49F1.5 /REP_DB=WormBase Gene ID /WP=CE17088 /TR=Q9GZE4 /GB=AAF99969.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068085(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068085 NCBI Caenorhabditis elegans F49F1.5 (F49F1.5) mRNA, complete cds. 11/11 None
GENEFINDER00000034603 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4119494:4123013:-1 11/11 None
F49F1.5 ENSEMBL cdna:known chromosome:CEL140:IV:4119453:4120383:-1 gene:F49F1.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4119499-4120389(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F49F1.5
Entrez Gene 186062 Entrez gene
SwissProt Q9GZE4 EMBL-EBI
WormBase CE17088 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF99963 Hypothetical protein F49F1.6 [Caenorhabditis elegans] ref|NP_500487.2| F49F1.6 [Caenorhabditis elegans] 1.0E-128
blast AAF99969 Hypothetical protein F49F1.5 [Caenorhabditis elegans] ref|NP_500486.1| F49F1.5 [Caenorhabditis elegans] 8.0E-86
blast AAF99969 Hypothetical protein F49F1.5 [Caenorhabditis elegans] ref|NP_500486.1| F49F1.5 [Caenorhabditis elegans] 5.0E-85
blast AAF99968 Hypothetical protein F49F1.1 [Caenorhabditis elegans] ref|NP_500485.1| F49F1.1 [Caenorhabditis elegans] 1.0E-54
blast CAE56272 Hypothetical protein CBG23917 [Caenorhabditis briggsae] 2.0E-22
blast CAB04402 Hypothetical protein F46B3.1 [Caenorhabditis elegans] ref|NP_507973.1| F46B3.1 [Caenorhabditis elegans] 7.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.3E-6
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.5E-7
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.5E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 6.9E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.8E-7
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.3E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.6E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.8E-12
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 7.3E-6
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.5E-7
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 5.5E-11
Sequence
Target Sequence 
>C. ELEGANS:180727_AT
gcctcattgttctattttttctgggtgcctgctcaagtcaatgcatggacacttcactgt
cttgccctgaccaagtgatcgcttgctttgaaccttctgtgcaaagtgaatgtccgtata
gctgtgccacttgtcagtttgacccgaatgagtgtgtggacagaaacacccaatgtcaga
ctctgaaggtcttctgtgatgatccgaagaatagcgagcaatgccaggaaagctgtggaa
tatgtgtgcccactagaaaaataacatctactttgcgaccggtacatgttggtacacaaa
cgactacagtaaagccaactagtcctaagacttcatcaacagcttcagaaccatgctacg
aaaatccaatttgtccgcattgggcggctaatggcttctgcaccaaccccttctactcgc
cggagcacagaaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCTCATTGTTCTATTTTTTCTGGG 568 281 26 Antisense
TGGGTGCCTGCTCAAGTCAATGCAT 648 553 47 Antisense
TGCATGGACACTTCACTGTCTTGCC 366 579 67 Antisense
AAGTGATCGCTTGCTTTGAACCTTC 427 159 98 Antisense
GAATGTCCGTATAGCTGTGCCACTT 687 331 133 Antisense
TGTGCCACTTGTCAGTTTGACCCGA 172 555 148 Antisense
AGAAACACCCAATGTCAGACTCTGA 297 73 187 Antisense
CTGAAGGTCTTCTGTGATGATCCGA 669 237 208 Antisense
TAACATCTACTTTGCGACCGGTACA 561 639 287 Antisense
CGCATTGGGCGGCTAATGGCTTCTG 675 247 401 Antisense
CTTCTACTCGCCGGAGCACAGAAAG 196 221 435 Antisense
Sequence Source Affymetrix Proprietary Database