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[180726_s_at]

Description F44D12.2 /REP_DB=WormBase Gene ID /WP=CE23727 /TR=Q20392 /GB=CAA92598.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 04      4grp_high_8_8 > 44      6grp_highest_nonorm > 70      6grp_total > 14      4grp_total > 52      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 347
1 2 3 4 range
A 366.5
(3)
P 		344.8
(2.4)
P 		334.5
(2.2)
P 		136.2
(-3)
A 		230
B 291.4
(-0.1)
P 		468.5
(3)
P 		304.7
(0.2)
P 		121.3
(-3)
P 		347
C 381.6
(1.5)
P 		465.1
(3)
P 		352.8
(1)
P 		124.5
(-3)
P 		341
avr 346.5
(1.4) 		426.1
(3) 		330.7
(1.1) 		127.3
(-3) 		299

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 365
1 2 3 4 5 6 range
A 432.2
(3)
P 		402.9
(2.3)
P 		377.2
(1.7)
P 		235.5
(-1.5)
P 		200.9
(-2.3)
P 		170.1
(-3)
P 		262
B 481
(3)
P 		361.5
(1)
P 		222
(-1.4)
P 		305.2
(0)
P 		252.8
(-0.9)
P 		127.9
(-3)
P 		353
C 431.7
(3)
P 		349.9
(1.4)
P 		317.3
(0.8)
P 		300.1
(0.5)
P 		219.1
(-1)
P 		116.2
(-3)
P 		316
avr 448.3
(3) 		371.4
(1.5) 		305.5
(0.2) 		280.3
(-0.2) 		224.3
(-1.3) 		138.1
(-3) 		310
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 114
1 2 3 4 range
A 133.4
(3)
P 		74.8
(-0.1)
P 		87.4
(0.6)
P 		19.9
(-3)
A 		114
B 92.9
(1.5)
P 		115.7
(3)
P 		70.6
(-0)
P 		25.9
(-3)
P 		90
C 126.3
(2.6)
P 		133.7
(3)
P 		123
(2.4)
P 		21
(-3)
P 		113
avr 117.5
(3) 		108.1
(2.4) 		93.7
(1.5) 		22.3
(-3) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 79.7
(-0.7)
P 		143.3
(3)
P 		82.1
(-0.6)
P 		59.7
(-1.9)
P 		60.6
(-1.9)
P 		41.4
(-3)
P 		102
B 167.4
(3)
P 		120.3
(0.6)
P 		53.9
(-2.7)
P 		80.8
(-1.3)
P 		102.7
(-0.2)
P 		47.7
(-3)
P 		120
C 160
(3)
P 		106.8
(0.4)
P 		80.8
(-0.9)
P 		60.3
(-1.9)
P 		70.9
(-1.4)
P 		38.9
(-3)
P 		121
avr 135.7
(3) 		123.5
(2.2) 		72.3
(-1.1) 		66.9
(-1.4) 		78.1
(-0.7) 		42.7
(-3) 		93
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 180726_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9416
Sequence Type Exemplar sequence
Representative Public ID F44D12.2 NCBI
Target Description F44D12.2 /REP_DB=WormBase Gene ID /WP=CE23727 /TR=Q20392 /GB=CAA92598.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069405(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069405 NCBI Caenorhabditis elegans F44D12.2 (F44D12.2) mRNA, complete cds. 11/11 None
GENEFINDER00000020525 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10014217:10016558:-1 11/11 None
F44D12.2 ENSEMBL cdna:known chromosome:CEL140:IV:10014058:10016299:-1 gene:F44D12.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000020509 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10014222-10016305(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F44D12.2
Entrez Gene 177861 Entrez gene
SwissProt Q20392 EMBL-EBI
WormBase CE23727 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92598 Hypothetical protein F44D12.2 [Caenorhabditis elegans] ref|NP_501806.1| F44D12.2 [Caenorhabditis elegans] 0.0
blast CAE59945 Hypothetical protein CBG03432 [Caenorhabditis briggsae] 1.0E-177
blast CAE59945 Hypothetical protein CBG03432 [Caenorhabditis briggsae] 1.0E-166
blast CAA99776 Hypothetical protein C15C8.5 [Caenorhabditis elegans] ref|NP_506188.1| C15C8.5 [Caenorhabditis elegans] 1.0E-18
blast CAA99776 Hypothetical protein C15C8.5 [Caenorhabditis elegans] ref|NP_506188.1| C15C8.5 [Caenorhabditis elegans] 2.0E-15
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501806.1 1 319-341
F44D12.2 1 319-341
GENEFINDER00000020525 1 347-369
Sequence
Target Sequence 
>C. ELEGANS:180726_S_AT
gtgccaattacacttggtccaccaaagactgcaataaggagaatgaatttgactttttcc
caactctaacagttttcaatttttatcctggaacatgcgtatggctcaactatggaggtc
aaccggacacacatgcatgttatggttctgattctctcgacacaacattagtctttgacg
tgacaactgctacaaccgaatgccacgtggattattttaatccacgtcttccatatgtta
agtccggaagttcttgtattggacagttctgtttcataagtgcgacttcccgtggagaag
tgttcagaggttgtgttaacagtgtgactgtcgagggtgccactccgttgaagattggct
acacccgtgcatacactggcttagagcaatggatttgtgatcaatcttactgtaatgctg
atctcaaatcagctgaactctcttggccacctgagctatatttatacagaaatatatcaa
atctccgagagttcaatgttttctacatcgactccgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCCAATTACACTTGGTCCACCAA 228 477 455 Antisense
TTGACTTTTTCCCAACTCTAACAGT 541 703 503 Antisense
GTCAACCGGACACACATGCATGTTA 618 463 572 Antisense
TGACGTGACAACTGCTACAACCGAA 684 567 630 Antisense
TTTTAATCCACGTCTTCCATATGTT 285 675 669 Antisense
TCATAAGTGCGACTTCCCGTGGAGA 11 621 728 Antisense
AGTGTGACTGTCGAGGGTGCCACTC 288 59 775 Antisense
CACTCCGTTGAAGATTGGCTACACC 263 145 795 Antisense
CACCCGTGCATACACTGGCTTAGAG 639 199 816 Antisense
TCTCTTGGCCACCTGAGCTATATTT 282 613 893 Antisense
TCAATGTTTTCTACATCGACTCCGC 116 633 947 Antisense
Sequence Source Affymetrix Proprietary Database