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[180723_at]

Description T22B2.2 /REP_DB=WormBase Gene ID /WP=CE13930 /TR=O45194 /GB=AAC04441.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 29      4grp_total > 18      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 95
1 2 3 4 range
A 16.5
(-2.9)
A 		32.4
(-0.8)
A 		15.8
(-3)
A 		61.4
(3)
A 		46
B 25.8
(-3)
A 		48.3
(2.8)
A 		37.6
(0)
A 		49.2
(3)
A 		23
C 5.1
(-3)
A 		12
(-2.6)
A 		11.2
(-2.6)
A 		100.1
(3)
A 		95
avr 15.8
(-3) 		30.9
(-1.3) 		21.5
(-2.4) 		70.2
(3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 39.9
(0.4)
A 		33.3
(-0.2)
A 		32.9
(-0.3)
A 		5.1
(-3)
A 		66.2
(3)
A 		18.8
(-1.7)
A 		61
B 20.1
(0.1)
A 		12.6
(-2)
A 		9.2
(-3)
A 		10.7
(-2.6)
A 		30
(3)
A 		28.1
(2.5)
A 		21
C 28.9
(0.1)
A 		51.2
(3)
A 		46.5
(2.4)
A 		33.7
(0.7)
A 		21.8
(-0.8)
A 		4.9
(-3)
A 		46
avr 29.6
(0.4) 		32.4
(1.2) 		29.5
(0.4) 		16.5
(-3) 		39.3
(3) 		17.3
(-2.8) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 6
(-0.7)
A 		7
(0.6)
A 		4.1
(-3)
A 		9
(3)
A 		5
B 8.2
(-3)
A 		11.9
(3)
A 		8.7
(-2.2)
A 		10.5
(0.7)
A 		4
C 1.7
(-3)
A 		3.4
(-2.3)
A 		3.9
(-2.1)
A 		16.9
(3)
A 		15
avr 5.3
(-3) 		7.4
(-1.1) 		5.6
(-2.7) 		12.1
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 7.4
(-1)
A 		11.8
(0.4)
A 		7.2
(-1.1)
A 		1.3
(-3)
A 		20
(3)
A 		4.6
(-1.9)
A 		19
B 7
(-0.1)
A 		4.2
(-1.8)
A 		2.2
(-3)
A 		2.8
(-2.6)
A 		12.2
(3)
A 		10.5
(2)
A 		10
C 10.7
(0.9)
A 		15.6
(3)
A 		11.8
(1.4)
A 		6.8
(-0.8)
A 		7
(-0.7)
A 		1.6
(-3)
A 		14
avr 8.4
(0) 		10.5
(1.4) 		7.1
(-0.8) 		3.6
(-3) 		13.1
(3) 		5.6
(-1.7) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 180723_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18130
Sequence Type Exemplar sequence
Representative Public ID T22B2.2 NCBI
Target Description T22B2.2 /REP_DB=WormBase Gene ID /WP=CE13930 /TR=O45194 /GB=AAC04441.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076278(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076278 NCBI Caenorhabditis elegans T22B2.2 (T22B2.2) mRNA, complete cds. 11/11 None
GENEFINDER00000014689 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:3921046:3923580:1 11/11 None
T22B2.2 ENSEMBL cdna:known chromosome:CEL140:X:3921661:3923211:1 gene:T22B2.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000014685 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:3921661-3923212(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T22B2.2
Entrez Gene 188717 Entrez gene
SwissProt O45194 EMBL-EBI
WormBase CE13930 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC04441 Hypothetical protein T22B2.2 [Caenorhabditis elegans] ref|NP_508679.1| T22B2.2 [Caenorhabditis elegans] 1.0E-103
blast AAC04441 Hypothetical protein T22B2.2 [Caenorhabditis elegans] ref|NP_508679.1| T22B2.2 [Caenorhabditis elegans] 6.0E-76
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508679.1 4 40-57,64-83,133-155,160-182
T22B2.2 4 40-57,64-83,133-155,160-182
Sequence
Target Sequence 
>C. ELEGANS:180723_AT
ggagctggagccttgaagtttgattccaaattagaaaaattcactaagataatttattgg
ggagttatcaccgcgtgtcatttattccctgcagtttttcctcaccgatactccatcaaa
attatccccaatgcatgtatctccctttccggagcagtttatgctgctcaactggctgtt
gtttcaaaagctgaatttgaagattacattaatccaatggattctcaagaaacttattcc
aaacaaaatcgagcaaccaacaacagaatgaaaatgccacaatgttttgaattggctttc
aaactcatatcttttatcaaatgggattttgtaaccgatttgttcgtcttgagtatactt
acctcagctcttattggccaatgctcaggatgggccaactctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGCTGGAGCCTTGAAGTTTGATT 240 521 82 Antisense
TATTGGGGAGTTATCACCGCGTGTC 363 663 136 Antisense
TTATCACCGCGTGTCATTTATTCCC 502 679 146 Antisense
TCCTCACCGATACTCCATCAAAATT 339 593 180 Antisense
AATTATCCCCAATGCATGTATCTCC 5 177 201 Antisense
TATCTCCCTTTCCGGAGCAGTTTAT 372 659 219 Antisense
ATGCTGCTCAACTGGCTGTTGTTTC 665 41 242 Antisense
ATTGGCTTTCAAACTCATATCTTTT 658 3 372 Antisense
GTAACCGATTTGTTCGTCTTGAGTA 548 461 412 Antisense
CTTACCTCAGCTCTTATTGGCCAAT 25 223 439 Antisense
CAATGCTCAGGATGGGCCAACTCTG 97 183 460 Antisense
Sequence Source Affymetrix Proprietary Database